Viability of Beauveria bassiana Spores Produced from Decanter Cake and Biohydrogen Effluent under Different Temperature Storage
Abstract
Beauveria bassiana produced from solid medium using the optimized decanter cake and liquid medium using biohydrogen effluent was stored under temperature of 4±2 and 30±2 0C to evaluate its viability based on spore germination percentage (SGP) in the laboratory interval 10 days for 90 days. The findings showed that B. bassiana produced from solid medium and stored under temperature of 4±2 0C gave the SGP reduction from 76.10 % to 34.50 % (average reduction: 0.46 % per day) while these spores which were stored under temperature of 30±2 0C gave the SGP reduction from 76.20 % to 32.00 % (average reduction: 0.49 % per day). In addition, B. bassiana produced from liquid medium using biohydrogen effluent was stored under temperature of 4±2 0C gave the SGP reduction from 75.50 % to 31.00 % (average reduction: 0.49 % per day) and its storage under 30±2 0C gave the SGP reduction 74.50 % to 27.00 % (average reduction: 0.53 % per day). This result indicated that B. bassiana which was produced from solid medium and stored under temperature of 4±2 0C exhibited higher fungal viability than produced from liquid medium and stored under temperature of 30±2 0C by 1.15 folds. Keywords: decanter cake, viability, Beauveria bassiana, biohydrogen effluent, storageReferences
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Amer, M. M., El-sayed, T. T., Bakheit, H. K., Mostafa, S. A., & El-sayed, Y.A. (2008). Pathogenicity and genetic variability of five entomopathogenic fungi against Spodoptera littoralis. Research Journal of Agriculture and Biological Sciences, 4, 354–367.
Aregger, E. (1992). Conidia production of the fungus Beauveria brongniartii on barley and quality evaluation during storage at 2°C. Journal of Invertibrate Pathology, 59, 2-10.
Blanford, S., Jenkins, N. E., Christian, R., Chan, B. H.K., Nardini, L., Osae, M., Koekemoer, L., Coetzee, M., Read, A. F., & Thomas, M. (2012). Storage and persistence of a candidate fungal biopesticide for use against adult malaria vectors. Malaria Journal, 11, 1-14.
Borman, A. M., Szekely, A., Campbell, C. K., & Johnson, E. M. (2006). Evaluation of the viability of pathogenic filamentous fungi after prolonged storage in sterile water and review of recent published studies on storage methods. Mycopathologia, 161, 361–368.
Chong-Rodríguez, M. J., Maldonado-Blanco, M. G., Hernández-Escareño, J. J., Galán-Wong, L. J.,& Sandoval-Coronado, C. F. (2011). Study of Beauveria bassiana growth, blastospore yield, desiccation-tolerance, viability and toxic activity using different liquid media. African Journal of Biotechnology, 10, 5736-5742.
Daoust, R. A., & Roberts, D. W. (1983). Studies on the prolonged storage of Metarhizium anisopliae conidia: Effect of temperature and relative humidity on conidial viability and virulence against mosquito. Journal of Invertebrate Pathology, 41, 143-150.
Godonou, I., James, B., Atcha-Ahowé, C., Vodouhè, S., Kooyman, C., Ahanchédé, A., & Korie, S. (2009). Potential of Beauveria bassiana and Metarhizium anisopliae isolates from Benin to control Plutella xylostella L. (Lepidoptera: Plutellidae). Crop Protection, 28, 220–224.
Haron, K., Mohammed, A. T., Halim, R. M., & Din, A. K. (2008). Palm-based bio-fertilizer from decanter cake and boiler ash of palm oil mill. Malaysian Palm Oil Board (MPOB) Information Series (MPOB TT No. 412), 14.
Hegedus, D. D, Bidochka, M. J., & Khachatourians, G. G. (1990). Beauveria bassiana submerged conidia production in a defined medium containing chitin, two hexosamines or glucose. Applied Microbiology and Biotechnology, 33, 641-647.
Hong, T. D., Ellis, R. H., & Moore, D. (1997). Development of a model to predict the effect of temperature and moisture for fungal spore longevity. Annual of Botany, 79, 121-128.
Hong, T. D., Gunn, J., Ellis, R. H., Jenkins, N. E., & Moore, D. (2001). The effect of storage environment on the longevity of conidia of Beauveria bassiana. Mycological Research, 105, 597-602.
Jackson, M. A. (1997). Optimizing nutritional conditions for the liquid culture production of effective fungal biological control agents. Journal of Industrial Microbiology and Biotechnology, 19, 180-187.
Juntaraniyom, T. (2008). Zero waste process of palm oil. Journal of Hatyai Academic, 2, 159-164. (in Thai)
Kassa, A., Brownbridge, M., Pakker, B. L.,Skinner, M., Gouli, V., Gouli, S., Guo, M., Lee, F., & Hata, T. (2008). Whey for mass production of Beauveria bassiana and Metarhizium anisopliae. Mycological Research, 112, 583-591.
Kivan, M. (2007). Pathogenicity of entomopathogenic fungi, Beauveria bassiana and Metarhizium anisopliae var anisoplia (Deuteromycotina: Hyphomycetes) against Eurygaster integriceps (Heteroptera: Scutelleridae) Entomologia Generalis, 30, 63-69.
Kruawal, K., Sacher, F., Werner, A., Müller, J., & Knepper, T. P. (2005). Chemical water quality in Thailand and its impacts on drinking water production in Thailand. Science of the Total Environment, 340, 57-70.
Lohmeyer, K.H., & Miller, J. A. (2006). Pathogenicity of three formulations of entomopathogenic fungi for control of adult Haematobia irritant (Diptera: Muscidae). Journal of Economic Entomology, 99, 1943-1947.
Magan, N. Ramirez, M. L., & Chulze, S. N. (2004). Impact of osmotic and matric water stress on germination, growth, mycelial water potentials and endogenous accumulation of sugars and sugar alcohols in Fusarium graminearum. Mycologia. 96, 470-478.
Oliveira, I., Pereira, J. A., Bento, A., & Baptista, P. (2011). Viability of Beauveria bassiana isolates after storage under several preservation methods. Annual Microbiology, 61, 339-344.
Pasarell, L., & McGinnis, M. R.(1992). Viability of fungal cultures maintained at -70 degree C. Journal of Clinical Microbiology. 30, 1000-1004.
Petlamul, W., & Prasertsan, P. (2012). Evaluation of strains of Metarhizium anisopliae and Beauveria bassiana against Spodoptera litura on the basis of their virulence, germination rate, spores production, radial growth and enzyme activity. Mycobiology, 40, 111-116.
Petlamul, W., & Prasertsan, P. (2014a). Spore production of an entomopathogenic fungus Beauveria bassiana BNBCRC for biocontrol: response surface optimization of medium using decanter cake from palm oil mill. Journal of the Korean Society for Applied Biological Chemistry, 57, 201-208.
Petlamul, W., & Prasertsan, P. (2014b). Medium optimization for production Beauveria bassiana BNBCRC spores from biohydrogen effluent of palm oil mill using taguchi design. International Journal of Bioscience Biochemistry and Bioinformatics, 4, 105-110.
Petlamul, W., & Prasertsan, P. (2015). Application of Response Surface Methodology for Spore Production Optimization of Beauveria bassiana Using Biohydrogen Effluent-Based Medium from Palm Oil Mill. The Proceeding of Kasetsart University Annual Conference, 53, 59-66.
Pham, T. A., Kim, J. J., Kim, S. G., & Kim, K. (2009). Production of spore of entomopathogenic Beauveria bassiana in a submerged batch culture. Mycobiology. 37, 218-224.
Razak, M. N. A., Ibrahim, M. F., Yee, P. L., Hassan, M. A., & Abd-Aziz, S. (2012). Utilization of oil palm decanter cake for cellulase and polyoses production. Biotechnology and Bioprocess Engineering, 17, 547-555.
Rombach, M. C., Humber, R. A., & Roberts, D. W. (1987). Metarhizium flavoviride var. minus var. nov., a pathogen of plant and leaf hoppers on rice in the Philippines and Solomon Islands. Mycotaxon, 27, 87-92.
Soundarapandian, P., & Chandra, R. (2007). Mass production of entomopathogenic fungus Metarhizium anisopliae (Deuteromycota; Hyphomycetes) in the laboratory. Research Journal of Microbiology, 2, 690-695.
Thai-PAN (Warning network of chemical pesticides). (2011). Documents of the Conference to monitor agricultural chemicals. Retrieved March 4, 2015, from http://www.thaipan.org/document.
Ubon, W., Cheewasedtham, W., Prasertsongskun, S., & Tjell, J. C. (2007). Potential of Organic residues from concentrated latex, fish processing and palm oil industries in the preparation of planting materials for garden grass. In. Proceeding the 9th Symposium on Graduate Research of KKU, KonKaen University.
Wakefield, M. E. (2006). Factors affecting storage insect susceptibility to the entomopathogenic fungus Beauveria bassiana. Alternative Methods to Chemical Control, 9, 855-862.
Xian-shi, Zhong, Y.X., & Lin-lin, X. (2004). Specific spoilage organisms from aquatic product and prediction & prolongation of the shelf life.Chinese Academy of Fisheries Sciences. Shanghai: East China Sea Fisheries Research Institute
Xing, W., Dong-jie, N., Xiao-shuang., Y., & You-cai, Z. (2008). Optimization of methane fermentation from effluent of bio-hydrogen fermentation process using response surface methodology. Bioresource Technology. 99, 4292-4299.
Yahya, A., Sye, C. P., Ishola, T. A., & Suryanto, H. (2010). Effect of adding palm palm oil mill decanter cake slurry with regular turning operation on the composting process and quality of compost from oil palm empty fruit bunches. Bioresource Technology, 101, 8736-8741.
Zabriskie, D. W., Armiger, W. B., Phillips, D. H., & Albano, P. A. (1999). Trader´s guide to fermentation media formulation. 5th edition. Traders Protein. Tennessee USA.
Amer, M. M., El-sayed, T. T., Bakheit, H. K., Mostafa, S. A., & El-sayed, Y.A. (2008). Pathogenicity and genetic variability of five entomopathogenic fungi against Spodoptera littoralis. Research Journal of Agriculture and Biological Sciences, 4, 354–367.
Aregger, E. (1992). Conidia production of the fungus Beauveria brongniartii on barley and quality evaluation during storage at 2°C. Journal of Invertibrate Pathology, 59, 2-10.
Blanford, S., Jenkins, N. E., Christian, R., Chan, B. H.K., Nardini, L., Osae, M., Koekemoer, L., Coetzee, M., Read, A. F., & Thomas, M. (2012). Storage and persistence of a candidate fungal biopesticide for use against adult malaria vectors. Malaria Journal, 11, 1-14.
Borman, A. M., Szekely, A., Campbell, C. K., & Johnson, E. M. (2006). Evaluation of the viability of pathogenic filamentous fungi after prolonged storage in sterile water and review of recent published studies on storage methods. Mycopathologia, 161, 361–368.
Chong-Rodríguez, M. J., Maldonado-Blanco, M. G., Hernández-Escareño, J. J., Galán-Wong, L. J.,& Sandoval-Coronado, C. F. (2011). Study of Beauveria bassiana growth, blastospore yield, desiccation-tolerance, viability and toxic activity using different liquid media. African Journal of Biotechnology, 10, 5736-5742.
Daoust, R. A., & Roberts, D. W. (1983). Studies on the prolonged storage of Metarhizium anisopliae conidia: Effect of temperature and relative humidity on conidial viability and virulence against mosquito. Journal of Invertebrate Pathology, 41, 143-150.
Godonou, I., James, B., Atcha-Ahowé, C., Vodouhè, S., Kooyman, C., Ahanchédé, A., & Korie, S. (2009). Potential of Beauveria bassiana and Metarhizium anisopliae isolates from Benin to control Plutella xylostella L. (Lepidoptera: Plutellidae). Crop Protection, 28, 220–224.
Haron, K., Mohammed, A. T., Halim, R. M., & Din, A. K. (2008). Palm-based bio-fertilizer from decanter cake and boiler ash of palm oil mill. Malaysian Palm Oil Board (MPOB) Information Series (MPOB TT No. 412), 14.
Hegedus, D. D, Bidochka, M. J., & Khachatourians, G. G. (1990). Beauveria bassiana submerged conidia production in a defined medium containing chitin, two hexosamines or glucose. Applied Microbiology and Biotechnology, 33, 641-647.
Hong, T. D., Ellis, R. H., & Moore, D. (1997). Development of a model to predict the effect of temperature and moisture for fungal spore longevity. Annual of Botany, 79, 121-128.
Hong, T. D., Gunn, J., Ellis, R. H., Jenkins, N. E., & Moore, D. (2001). The effect of storage environment on the longevity of conidia of Beauveria bassiana. Mycological Research, 105, 597-602.
Jackson, M. A. (1997). Optimizing nutritional conditions for the liquid culture production of effective fungal biological control agents. Journal of Industrial Microbiology and Biotechnology, 19, 180-187.
Juntaraniyom, T. (2008). Zero waste process of palm oil. Journal of Hatyai Academic, 2, 159-164. (in Thai)
Kassa, A., Brownbridge, M., Pakker, B. L.,Skinner, M., Gouli, V., Gouli, S., Guo, M., Lee, F., & Hata, T. (2008). Whey for mass production of Beauveria bassiana and Metarhizium anisopliae. Mycological Research, 112, 583-591.
Kivan, M. (2007). Pathogenicity of entomopathogenic fungi, Beauveria bassiana and Metarhizium anisopliae var anisoplia (Deuteromycotina: Hyphomycetes) against Eurygaster integriceps (Heteroptera: Scutelleridae) Entomologia Generalis, 30, 63-69.
Kruawal, K., Sacher, F., Werner, A., Müller, J., & Knepper, T. P. (2005). Chemical water quality in Thailand and its impacts on drinking water production in Thailand. Science of the Total Environment, 340, 57-70.
Lohmeyer, K.H., & Miller, J. A. (2006). Pathogenicity of three formulations of entomopathogenic fungi for control of adult Haematobia irritant (Diptera: Muscidae). Journal of Economic Entomology, 99, 1943-1947.
Magan, N. Ramirez, M. L., & Chulze, S. N. (2004). Impact of osmotic and matric water stress on germination, growth, mycelial water potentials and endogenous accumulation of sugars and sugar alcohols in Fusarium graminearum. Mycologia. 96, 470-478.
Oliveira, I., Pereira, J. A., Bento, A., & Baptista, P. (2011). Viability of Beauveria bassiana isolates after storage under several preservation methods. Annual Microbiology, 61, 339-344.
Pasarell, L., & McGinnis, M. R.(1992). Viability of fungal cultures maintained at -70 degree C. Journal of Clinical Microbiology. 30, 1000-1004.
Petlamul, W., & Prasertsan, P. (2012). Evaluation of strains of Metarhizium anisopliae and Beauveria bassiana against Spodoptera litura on the basis of their virulence, germination rate, spores production, radial growth and enzyme activity. Mycobiology, 40, 111-116.
Petlamul, W., & Prasertsan, P. (2014a). Spore production of an entomopathogenic fungus Beauveria bassiana BNBCRC for biocontrol: response surface optimization of medium using decanter cake from palm oil mill. Journal of the Korean Society for Applied Biological Chemistry, 57, 201-208.
Petlamul, W., & Prasertsan, P. (2014b). Medium optimization for production Beauveria bassiana BNBCRC spores from biohydrogen effluent of palm oil mill using taguchi design. International Journal of Bioscience Biochemistry and Bioinformatics, 4, 105-110.
Petlamul, W., & Prasertsan, P. (2015). Application of Response Surface Methodology for Spore Production Optimization of Beauveria bassiana Using Biohydrogen Effluent-Based Medium from Palm Oil Mill. The Proceeding of Kasetsart University Annual Conference, 53, 59-66.
Pham, T. A., Kim, J. J., Kim, S. G., & Kim, K. (2009). Production of spore of entomopathogenic Beauveria bassiana in a submerged batch culture. Mycobiology. 37, 218-224.
Razak, M. N. A., Ibrahim, M. F., Yee, P. L., Hassan, M. A., & Abd-Aziz, S. (2012). Utilization of oil palm decanter cake for cellulase and polyoses production. Biotechnology and Bioprocess Engineering, 17, 547-555.
Rombach, M. C., Humber, R. A., & Roberts, D. W. (1987). Metarhizium flavoviride var. minus var. nov., a pathogen of plant and leaf hoppers on rice in the Philippines and Solomon Islands. Mycotaxon, 27, 87-92.
Soundarapandian, P., & Chandra, R. (2007). Mass production of entomopathogenic fungus Metarhizium anisopliae (Deuteromycota; Hyphomycetes) in the laboratory. Research Journal of Microbiology, 2, 690-695.
Thai-PAN (Warning network of chemical pesticides). (2011). Documents of the Conference to monitor agricultural chemicals. Retrieved March 4, 2015, from http://www.thaipan.org/document.
Ubon, W., Cheewasedtham, W., Prasertsongskun, S., & Tjell, J. C. (2007). Potential of Organic residues from concentrated latex, fish processing and palm oil industries in the preparation of planting materials for garden grass. In. Proceeding the 9th Symposium on Graduate Research of KKU, KonKaen University.
Wakefield, M. E. (2006). Factors affecting storage insect susceptibility to the entomopathogenic fungus Beauveria bassiana. Alternative Methods to Chemical Control, 9, 855-862.
Xian-shi, Zhong, Y.X., & Lin-lin, X. (2004). Specific spoilage organisms from aquatic product and prediction & prolongation of the shelf life.Chinese Academy of Fisheries Sciences. Shanghai: East China Sea Fisheries Research Institute
Xing, W., Dong-jie, N., Xiao-shuang., Y., & You-cai, Z. (2008). Optimization of methane fermentation from effluent of bio-hydrogen fermentation process using response surface methodology. Bioresource Technology. 99, 4292-4299.
Yahya, A., Sye, C. P., Ishola, T. A., & Suryanto, H. (2010). Effect of adding palm palm oil mill decanter cake slurry with regular turning operation on the composting process and quality of compost from oil palm empty fruit bunches. Bioresource Technology, 101, 8736-8741.
Zabriskie, D. W., Armiger, W. B., Phillips, D. H., & Albano, P. A. (1999). Trader´s guide to fermentation media formulation. 5th edition. Traders Protein. Tennessee USA.
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2016-02-01
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