High-Performance Liquid Chromatography Method for Simultaneous Determination of Paracetamol and Tramadol in Tablet Using Chromolith C18 Column

Abstract

In this work, a simple and rapid high-performance liquid chromatography was developed and optimized for simultaneous determination of paracetamol and tramadol in tables. The chromatographic separations were carried out on Chromolith C18 (25 mm ´ 3 mm i.d.) using a 10 mM phosphate buffer (pH 3.0)-acetonitrile (95:5, v/v) in a gradient elution mobile phase at the flow rate of 1.5 mL/min and detection of 215 nm. Under the optimal conditions, the analysis time was less than 1 min with re-equilibration time of approximately 0.5 min. The calibration curve (5.0 – 200.0 mg/L) was found to have a linear relationship (r2 > 0.998) over the analytical range of 5.0 to 200.0 mg/L. The detection limits of paracetamol and tramadol were 0.03 and 0.05, respectively. The quantitation limits of paracetamol and tramadol were 0.10 and 0.15 mg/L, respectively. Precision in terms of percent relative standard deviation ranged from 1.1% to 2.9%, and accuracy was between 98.4% and 101.1%. The proposed method was successfully applied to the determination of paracetamol and tramadol in tables. Keywords :  high performance liquid chromatography ; paracetamol ; tramadol ; chromolith C18