In vitro Shoot Induction and Shoot Multiplication of Asparagus (Asparagus officinalis)
Abstract
From shoot induction and shoot multiplication of Asparagus (Asparagus officinalis) in vitro was launched by cultivating 1.5±0.2 cm lateral bud on Murashige and Skoog (MS, 1962) media with the addition of plant growth regulators in cytokinin group i.e. BAP at 0, 0.5, 1.0, 1.5 and 2.0 mg/L. After culturing for 4 weeks, it was found that MS media with BAP in all concentration could induce shoot as well. In addition, some lateral bud explants could be developed into callus. Obviously, MS media with 0.5 mg/L BAP had the highest shoot length and statistically different from other experiments. After that, shoot tip explants were obtained from aseptic seedlings culturing on MS media supplemented with BAP or kinetin alone or together with NAA at 0.1, 0.5, 1.0 mg/L. The result showed that MS medium supplemented with 0.5 mg/L BAP or 1.0 mg/L kinetin alone or addition of 0.5 mg/L BAP with 0.1 mg/L NAA could induce number of shoot well. For root induction, shoot tip explants were cultured on MS media supplemented with plant growth regulators in auxin group including NAA, IAA and IBA at different concentration for 8 weeks. The result revealed that MS media supplemented with 1.0 mg/L IBA was more effective in root induction of Asparagus while other experimental, no root formation. Keywords : shoot and root induction ; plant growth regulator ; Asparagus officinalis.References
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on shoot multiplication and IBA on root induction of Butea
monosperma (Lam.) Taub. In Proceeeding 48th Kasetsart
University Academic Conference. (pp. 31-38). Bangkok :
Kasetsart University.
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of Phalaenopsis Hybrid (Phalaenopsis Silky Moon). Master's
thesis. Silpakorn University.
Sargsyan, E., Vardanyan, A., Parsaeimehr, A. & Bekyan, A. (2015).
Elaboration of Asparagus officinalis L. by in vitro and hydroponic
combined method. American Journal of Microbiology and
Biotechnology, 2(6), 81–86.
Seniwong Na Ayudhya, W., & Sananpanichkul W. (2019). Thai
asparagus spread far and wide around the world by means of
Plant tissue culture. Retrieved March 15, 2022, from
https://www. technologychaoban.com/bullet-news-
today/article_74713.
Sujatha, M. & Reddy, T.P. (1998). Differential cytokinin effects on the
stimulation of in vitro shoot proliferation from meristematic
explants of castor. Plant Cell Reports ,17(6-7), 561–566.
Supinrach S., & Supinrach I. (2014). Effects of IBA and NAA on
Rooting for the Orchid Plantlet Rhynchostylis gigantea (Lindl.)
Ridl. ‘Cartoon’. Thai Science and Technology Journal, 22(4), 507–
514.
Taiz, L. and Zeiger, E. (2002). Plant Physiology. California: The
Benjamin/Cummings Publishing Company, Inc.
Thai Kasetsart. (2011). Diseases of asparagus. Retrieved March 5,
2022, from https://www.thaikasetsart.com.
Techapinyawat, S. (2001). Plant Physiology. Bangkok: Kasetsart
University.
Thongampai P. (2014). The use of plant root accelerators. Retrieved
March, 5,2022, from https://www.thaikasetsart.com.
Is the Most Effective Cytokinin on Shoot Multiplication from
Cucumber. Journal of Agricultural Science, 7(10), 159–165.
Anis, M., Faisal, M. & Singh, S.K. (2003). Micropropagation of
Mulberry (Morus alba) through in vitro culture
of shoot tip and nodal explants. Plant Tissue Culture,13, 47–51.
Borjian, L. & Arak, H. (2013). A study on the effect of different
concentration of plant hormones (BAP, NAA,
2, 4-D and Kinetin) on callus induction in Brassica napus. Intl.
Res. J. Appl. Basic. Sci,5,
519-521.
Bousserouel, S., Grandois, J., Gosse, F., Werner, D., Barth, S.W.,
Marchioni, E., Marescaux, J. & Raul, F.A.
(2013). Methanolic extract of white Asparagus shoots activates
trail apoptotic death pathway in human cancer cells and inhibits
colon carcinogenesis in a preclinical model. Int. J. of Oncology,
43(2), 394–404.
Huang, X. and Kong, L. (2006). Steroidal saponins from roots of Asparagus officinalis. Steroids, 71(2), 171–
176.
Inkaew W., & Sotthikul J. (2012). Influence of BA and TDZ on
Chompoophukha’s propagation under sterile
conditions. Chiang Mai: Department of Horticulture, Faculty of
Agriculture. Chiang Mai University.
Jang, D.S., Cuendet, M., Fong, H.H., Pezzuto, J.M. & Kinghorn, A.D.
(2004). Constituents of Asparagus officinalis evaluated for
inhibitory activity against cyclooxygenase-2. J. Agric. Food
Chem, 52, 2218–2222.
Kanjanapisut, K. (1987). Asparagus. Bangkok : Textbook Production
Center for Rural Areas.
Knowledge of Sustainable highland development. (2016). Asparagus.
Retrieved February 28, 2022, from
https://hkm.hrdi.or.th/knowledge/detail/85.
Makcayathorn M., Tantiwiwat S., & Na Nakorn M. (2012). Effect of BA
on shoot multiplication and IBA on root induction of Butea
monosperma (Lam.) Taub. In Proceeeding 48th Kasetsart
University Academic Conference. (pp. 31-38). Bangkok :
Kasetsart University.
Rojanawong T. (2006). Tissue culture and in vitro flowering induction
of Phalaenopsis Hybrid (Phalaenopsis Silky Moon). Master's
thesis. Silpakorn University.
Sargsyan, E., Vardanyan, A., Parsaeimehr, A. & Bekyan, A. (2015).
Elaboration of Asparagus officinalis L. by in vitro and hydroponic
combined method. American Journal of Microbiology and
Biotechnology, 2(6), 81–86.
Seniwong Na Ayudhya, W., & Sananpanichkul W. (2019). Thai
asparagus spread far and wide around the world by means of
Plant tissue culture. Retrieved March 15, 2022, from
https://www. technologychaoban.com/bullet-news-
today/article_74713.
Sujatha, M. & Reddy, T.P. (1998). Differential cytokinin effects on the
stimulation of in vitro shoot proliferation from meristematic
explants of castor. Plant Cell Reports ,17(6-7), 561–566.
Supinrach S., & Supinrach I. (2014). Effects of IBA and NAA on
Rooting for the Orchid Plantlet Rhynchostylis gigantea (Lindl.)
Ridl. ‘Cartoon’. Thai Science and Technology Journal, 22(4), 507–
514.
Taiz, L. and Zeiger, E. (2002). Plant Physiology. California: The
Benjamin/Cummings Publishing Company, Inc.
Thai Kasetsart. (2011). Diseases of asparagus. Retrieved March 5,
2022, from https://www.thaikasetsart.com.
Techapinyawat, S. (2001). Plant Physiology. Bangkok: Kasetsart
University.
Thongampai P. (2014). The use of plant root accelerators. Retrieved
March, 5,2022, from https://www.thaikasetsart.com.
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Published
2023-01-04
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