Vein Density, Number and Size of Interveinal Mesophyll Cells of Four Thai Cultivars of Rice (Oryza sativa L.)
Abstract
This research studied on leaf morphology and anatomy of four cultivars of rice, RD7, RD49, Pathumthani1, and Phatthalung. The objective of this research was to select the cultivar that its morphological and anatomical characters can be use in breeding program to higher photosynthetic efficiency of rice. Flag leaves from rice plant (60 day after sowing) were collected. The morphological and anatomical characteristics including vein density, interveinal length and number of interveinal-mesophyll and their size were determined. Vein density of four rice cultivars were in a range of 4.29-4.83 vein/mm. and the interveinal length were between 402-448 μm. The second layer of mesophyll underneath the bulliform cells at the interveinal position of four cultivars were in the ranges of 7-8 cells. The size of the mesophyll in width and length of the cells were in the ranges of 50-62 μm and 23-26 μm, respectively. In summary, the differ of vein density of rice leaves are from the cell length and number of interveinal mesophyll cells. Keywords : rice ; leaf anatomy ; vein density ; interveinal mesophyllReferences
Adachi, S., Nakae, T., Uchida, M., Soda, K., Takai, T., Oi, T., Yamamoto, T., Ookawa, T., Miyake, H., Yano, M., & Hirasawa, T. (2013). The mesophyll anatomy enhancing CO2 diffusion is a key traitfor improving rice photosynthesis. Journal of Experimental Botany, 60(4), 1061-1072.
Adams III, W.W., Cohu, C.M., Muller, O., & Demmig-Adamset, B. (2003). Foliar phloem infrastructure in support of photosynthesis. Frontiers in. Plant Science, doi: 10.3389/fpls.2013.00194.
Kajala, K., Covshoff, S., Karki, S., Woodfield, H., Tolley, B.J., Dionora, M., J.A., Mogu, R.T., Mabilangan, A.E., Danila, F.R., Hibberd, J.M., & Quick, W.P. (2011). Strategies for engineering a two-celled C4 photosynthetic pathway into rice. Journal of Experimental Botany, 10, 1-10.
Karki, S., Rizal, G. and Quick, W.P. (2013). Improvement of photosynthesis in rice (Oryza sativa L.) by inserting the C4 pathway. Rice, 6, 28.
Feldman, A.B., K., Murchie, E.H., Leung, H., Baraoidan, M., Coe, R., Yu, S.M., Lo, S.F., & Quick, W.P. (2013). Increasing leaf vein density by mutagenesis: Laying the foundations for C4 rice. PLOS, 9(4), 1-9.
Feldman, A.B., Leung, H., Baraoidan, M., Elmido-Mabilangan, A., Canicosa, I., Quick, W.P., Sheehy, J., & Murchie, E.H. (2017). Increasing leaf vein density via mutagenesis in rice results in an enhanced rate of photosynthesis, smaller cell sizes and can reduce interveinal mesophyll cell number. Frontiers in Plant Science, doi: 10.3389/fpls.2017.01883.
Gadal, N., Shrestha, J., Poudel, M.N., & Pokharel, B. (2019). A review on production status and growing environments of rice in Nepal and in the world. Archives of Agriculture and Environmental Science, 4(1), 83-87.
Mathan, J., Singh, A., & Ranjan, A. (2021) Sucrose transport and metabolism control carbon partitioning between stem and grain in rice. Journal of Experimental Botany, 72(12):4355-4372.
Kermanee, P. (2008). Techniques in Plant Tissue. Bangkok: Kasetsart University Press. (in Thai)
Smillie, I. R. A., Pyke, K. A., & Murchie, E. H. (2012). Variation in vein density and mesophyll cell architecture in a rice deletion mutant population. Journal of Experimental Botany, 63, 12.
Ueno, O., Kawano, Y., Wakayama, M., & Takeda, T. (2006). Leaf vascular systems in C3 and C4 grasses: A two-dimensional analysis, Annals of Botany, 97(4), 611–621.
Wang, G., Wu, Y., Ma, Li., Lin, Y., Hu, Y., Li, M., Li, W., Ding, Y., & Chen, L. (2021). Phloem loading in rice leaves depends strongly on the apoplastic pathway. Journal of Experimental Botany, 72(10), 3723–3738.
Adams III, W.W., Cohu, C.M., Muller, O., & Demmig-Adamset, B. (2003). Foliar phloem infrastructure in support of photosynthesis. Frontiers in. Plant Science, doi: 10.3389/fpls.2013.00194.
Kajala, K., Covshoff, S., Karki, S., Woodfield, H., Tolley, B.J., Dionora, M., J.A., Mogu, R.T., Mabilangan, A.E., Danila, F.R., Hibberd, J.M., & Quick, W.P. (2011). Strategies for engineering a two-celled C4 photosynthetic pathway into rice. Journal of Experimental Botany, 10, 1-10.
Karki, S., Rizal, G. and Quick, W.P. (2013). Improvement of photosynthesis in rice (Oryza sativa L.) by inserting the C4 pathway. Rice, 6, 28.
Feldman, A.B., K., Murchie, E.H., Leung, H., Baraoidan, M., Coe, R., Yu, S.M., Lo, S.F., & Quick, W.P. (2013). Increasing leaf vein density by mutagenesis: Laying the foundations for C4 rice. PLOS, 9(4), 1-9.
Feldman, A.B., Leung, H., Baraoidan, M., Elmido-Mabilangan, A., Canicosa, I., Quick, W.P., Sheehy, J., & Murchie, E.H. (2017). Increasing leaf vein density via mutagenesis in rice results in an enhanced rate of photosynthesis, smaller cell sizes and can reduce interveinal mesophyll cell number. Frontiers in Plant Science, doi: 10.3389/fpls.2017.01883.
Gadal, N., Shrestha, J., Poudel, M.N., & Pokharel, B. (2019). A review on production status and growing environments of rice in Nepal and in the world. Archives of Agriculture and Environmental Science, 4(1), 83-87.
Mathan, J., Singh, A., & Ranjan, A. (2021) Sucrose transport and metabolism control carbon partitioning between stem and grain in rice. Journal of Experimental Botany, 72(12):4355-4372.
Kermanee, P. (2008). Techniques in Plant Tissue. Bangkok: Kasetsart University Press. (in Thai)
Smillie, I. R. A., Pyke, K. A., & Murchie, E. H. (2012). Variation in vein density and mesophyll cell architecture in a rice deletion mutant population. Journal of Experimental Botany, 63, 12.
Ueno, O., Kawano, Y., Wakayama, M., & Takeda, T. (2006). Leaf vascular systems in C3 and C4 grasses: A two-dimensional analysis, Annals of Botany, 97(4), 611–621.
Wang, G., Wu, Y., Ma, Li., Lin, Y., Hu, Y., Li, M., Li, W., Ding, Y., & Chen, L. (2021). Phloem loading in rice leaves depends strongly on the apoplastic pathway. Journal of Experimental Botany, 72(10), 3723–3738.
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2022-09-20
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