Morphology and Growth Monitoring Methods of Aurantiochytrium sp. FIKU018 Cultivated in Flask and Bioreactor

Authors

  • Katsaya Khumrangsee Department of Biotechnology, Faculty of Science and Technology, Thammasat University
  • Theppanya Charoenrat Department of Biotechnology, Faculty of Science and Technology, Thammasat University
  • Supenya Chittapun Department of Biotechnology, Faculty of Science and Technology, Thammasat University

Abstract

The development of growth monitoring method is significant for the biomass production of Aurantiochytrium as a DHA resource. This study aimed to examine the growth measurement methods and morphological observation of Aurantiochytrium cultured in flasks and bioreactors. The batch cultivation of Aurantiochytrium sp. FIKU018 was carried on 50 mL of GYP broth in 250 mL flask. The cultivation was performed at 30 °C on 200 rpm rotary shaker under dark condition. Then, the fed-batch fermentation was examined in 22 L bioreactor contained 6 L GYP broth. The incubation was carried on 30 °C, pH 6.0, 450 rpm of agitation speed and 1.0 VVM of aeration rate. Samples were taken every 4 h for 72 h. In shaking flask, it was found that the batch cultivation of Aurantiochytrium sp. FIKU018 gave specific growth rate (SGR) and maximum dry cell weight (DCW) at 0.11 h-1 and 12.47 ± 0.70 g L-1, respectively. The fed-batch cultivation was performed in 22 L bioreactor contained 6 L of GYP broth. The result showed that cell cultivation during 0 - 20 h showed SGR and maximum DCW at 0.21 h-1 and 38.87 ± 0.71 g L-1, respectively. Fed-batch cultivation during 20 - 42 h and 42 – 72 h showed maximum DCW at 25.17 ± 0.23 g L-1 and 38.87 ± 0.71 g L-1, respectively. Aurantiochytrium sp. FIKU018 morphology changed according to growth and lipid accumulation phases. DCW can be used as growth parameters to monitor the growth of Aurantiochytrium sp. FIKU018 cultured both in flask and in bioreactor. Optical density and cell count can be used to tracking the growth of cells during 0 - 42 h in fed-batch cultivation in bioreactor.  Therefore, all growth parameters can be use as growth monitor method during the development of Aurantiochytrium sp. FIKU018 biomass production processes. Until the lipid accumulation phase, fed with broth containing no nitrogen source, DCW associated with morphology are acceptable parameters to monitor the cell growth. Keywords :  fed-batch cultivation ; optical density ; cell count ; dry cell weight ; specific growth rate

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Published

2022-01-10