Evaluation of Broth Disk Elution to Determine Colistin Susceptibility of Acinetobacter baumannii
Abstract
Acinetobacter baumannii is one of the major human pathogen causing nosocomial infections with highly drug-resistant to multiple classes of antibiotics. Antimicrobial susceptibility testing of colistin are more complex and high cost of commercial kit. In this study, we aimed to evaluate the colistin broth disk elution (CBDE) to determine in vitro colistin susceptibility against A. baumannii. A total of 98 isolates of A. baumannii were used in this study. Colistin MICs against A. baumannii were determined by CBDE and were compared with broth microdilution method. A very major error (VME), a major error (ME), categorical agreement (CA), essential agreement (EA), specificity, and sensitivity were analyzed. CBDE yield a CA and EA of 82.7 and 84.7%, respectively. Twelve VME (46.2.3%) and 5 ME (6.9%) were observed. Sensitivity and specificity of CBDE were 53.8 and 93.1%, respectively. CBDE was not suitable to detect colistin susceptibility of A. baumannii. Keywords : colistin ; broth disk elution ; Acinetobacter baumanniiReferences
Bianchini, H., Canigia, L. F., Predari, S. C., Rollet, R., Litterio, M., Berestein, P., et al. (1997). Broth disk elution method for anaerobic bacteria: a collaborative study to assess its reliability for clinical purposes. Anaerobe, 3(4), 225-231. doi:10.1006/anae.1997.0110
CLSI. (2018). Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically ( 10th ed.). Wayne,PA: Clinical and Laboratory Standards Institute.
CLSI. (2020). Performance standard for Antimicrobrail Susceptibility testing ( 30th ed.). Wayne,PA: Clinical and Laboratory Standards Institute.
EUCAST. ( 2019). Breakpoint tables for interpretation of MICs and zone diameters, version 9.0, 2019. Retrieved from https://www.eucast.org/fileadmin/src/media/PDFs/EUCAST_files/Breakpoint_tables/v_11.0_Breakpoint_Tables.pdf
Gonzalez-Villoria, A. M., & Valverde-Garduno, V. (2016). Antibiotic-resistant Acinetobacter baumannii increasing success remains a challenge as a nosocomial pathogen. J Pathog, 2016, 7318075. doi:10.1155/2016/7318075
Hawley, J. S., Murray, C. K., & Jorgensen, J. H. (2008). Colistin heteroresistance in acinetobacter and its association with previous colistin therapy. Antimicrobial agents and chemotherapy, 52(1), 351-352. doi:10.1128/AAC.00766-07
Higgins, P. G., Dammhayn, C., Hackel, M., & Seifert, H. (2010). Global spread of carbapenem-resistant Acinetobacter baumannii. J Antimicrob Chemother, 65(2), 233-238. doi:10.1093/jac/dkp428
Hindler, J. A., & Humphries, R. M. (2013). Colistin MIC variability by method for contemporary clinical isolates of multidrug-resistant Gram-negative bacilli. J Clin Microbiol, 51(6), 1678-1684. doi:10.1128/jcm.03385-12
Humphries, R. M., Green, D. A., Schuetz, A. N., Bergman, Y., Lewis, S., Yee, R., et al. (2019). Multi-center evaluation of colistin broth disk elution and colistin agar test: a report from the Clinical and Laboratory Standards Institute. . J Clin Microbiol, 57(11). doi:10.1128/jcm.01269-19
ISO. (2018). ISO 20776-2:2007, Clinical laboratory testing and in vitro diagnostic test systems- Susceptibility testing of infectious agents and evaluation of performance of antimicrobial susceptibility test devices Retrieved from https://www.iso.org/standard/41631.html.
Jorgensen, J. H., Redding, J. S., & Howell, A. W. (1986). Evaluation of broth disk elution methods for susceptibility testing of anaerobic bacteria with the newer beta-lactam antibiotics. J Clin Microbiol, 23(3), 545-550. doi:10.1128/jcm.23.3.545-550.1986
Karvanen, M., Malmberg, C., Lagerbäck, P., Friberg, L. E., & Cars, O. (2017). Colistin Is extensively lost during standard in vitro experimental conditions. Antimicrobial agents and chemotherapy, 61(11). doi:10.1128/aac.00857-17
Kon, H., Abramov, S., Amar Ben Dalak, M., Elmaliach, N., Schwartz, D., Carmeli, Y., et al. (2020). Performance of Rapid Polymyxin™ NP and Rapid Polymyxin™ Acinetobacter for the detection of polymyxin resistance in carbapenem-resistant Acinetobacter baumannii and Enterobacterales. J Antimicrob Chemother, 75(6), 1484-1490. doi:10.1093/jac/dkaa050
Matuschek, E., Åhman, J., Webster, C., & Kahlmeter, G. (2018). Antimicrobial susceptibility testing of colistin - evaluation of seven commercial MIC products against standard broth microdilution for Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, and Acinetobacter spp. Clin Microbiol Infect, 24(8), 865-870. doi:10.1016/j.cmi.2017.11.020
Nation, R. L., Garonzik, S. M., Thamlikitkul, V., Giamarellos-Bourboulis, E. J., Forrest, A., Paterson, D. L., et al. (2017). Dosing guidance for intravenous colistin in critically-ill patients. Clin Infect Dis, 64(5), 565-571. doi:10.1093/cid/ciw839
Qureshi, Z. A., Hittle, L. E., O'Hara, J. A., Rivera, J. I., Syed, A., Shields, R. K., et al. (2015). Colistin-resistant Acinetobacter baumannii: beyond carbapenem resistance. Clin Infect Dis, 60(9), 1295-1303. doi:10.1093/cid/civ048
Simner, P. J., Bergman, Y., Trejo, M., Roberts, A. A., Marayan, R., Tekle, T., et al. (2019). Two-site evaluation of the colistin broth disk elution test to determine colistin in vitro activity against Gram-negative bacilli. J Clin Microbiol, 57(2). doi:10.1128/jcm.01163-18
Tan, T. Y., & Ng, L. S. (2006). Comparison of three standardized disc susceptibility testing methods for colistin. J Antimicrob Chemother, 58(4), 864-867. doi:10.1093/jac/dkl330
Viehman, J. A., Nguyen, M. H., & Doi, Y. (2014). Treatment options for carbapenem-resistant and extensively drug-resistant Acinetobacter baumannii infections. Drugs, 74(12), 1315-1333. doi:10.1007/s40265-014-0267-8
CLSI. (2018). Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically ( 10th ed.). Wayne,PA: Clinical and Laboratory Standards Institute.
CLSI. (2020). Performance standard for Antimicrobrail Susceptibility testing ( 30th ed.). Wayne,PA: Clinical and Laboratory Standards Institute.
EUCAST. ( 2019). Breakpoint tables for interpretation of MICs and zone diameters, version 9.0, 2019. Retrieved from https://www.eucast.org/fileadmin/src/media/PDFs/EUCAST_files/Breakpoint_tables/v_11.0_Breakpoint_Tables.pdf
Gonzalez-Villoria, A. M., & Valverde-Garduno, V. (2016). Antibiotic-resistant Acinetobacter baumannii increasing success remains a challenge as a nosocomial pathogen. J Pathog, 2016, 7318075. doi:10.1155/2016/7318075
Hawley, J. S., Murray, C. K., & Jorgensen, J. H. (2008). Colistin heteroresistance in acinetobacter and its association with previous colistin therapy. Antimicrobial agents and chemotherapy, 52(1), 351-352. doi:10.1128/AAC.00766-07
Higgins, P. G., Dammhayn, C., Hackel, M., & Seifert, H. (2010). Global spread of carbapenem-resistant Acinetobacter baumannii. J Antimicrob Chemother, 65(2), 233-238. doi:10.1093/jac/dkp428
Hindler, J. A., & Humphries, R. M. (2013). Colistin MIC variability by method for contemporary clinical isolates of multidrug-resistant Gram-negative bacilli. J Clin Microbiol, 51(6), 1678-1684. doi:10.1128/jcm.03385-12
Humphries, R. M., Green, D. A., Schuetz, A. N., Bergman, Y., Lewis, S., Yee, R., et al. (2019). Multi-center evaluation of colistin broth disk elution and colistin agar test: a report from the Clinical and Laboratory Standards Institute. . J Clin Microbiol, 57(11). doi:10.1128/jcm.01269-19
ISO. (2018). ISO 20776-2:2007, Clinical laboratory testing and in vitro diagnostic test systems- Susceptibility testing of infectious agents and evaluation of performance of antimicrobial susceptibility test devices Retrieved from https://www.iso.org/standard/41631.html.
Jorgensen, J. H., Redding, J. S., & Howell, A. W. (1986). Evaluation of broth disk elution methods for susceptibility testing of anaerobic bacteria with the newer beta-lactam antibiotics. J Clin Microbiol, 23(3), 545-550. doi:10.1128/jcm.23.3.545-550.1986
Karvanen, M., Malmberg, C., Lagerbäck, P., Friberg, L. E., & Cars, O. (2017). Colistin Is extensively lost during standard in vitro experimental conditions. Antimicrobial agents and chemotherapy, 61(11). doi:10.1128/aac.00857-17
Kon, H., Abramov, S., Amar Ben Dalak, M., Elmaliach, N., Schwartz, D., Carmeli, Y., et al. (2020). Performance of Rapid Polymyxin™ NP and Rapid Polymyxin™ Acinetobacter for the detection of polymyxin resistance in carbapenem-resistant Acinetobacter baumannii and Enterobacterales. J Antimicrob Chemother, 75(6), 1484-1490. doi:10.1093/jac/dkaa050
Matuschek, E., Åhman, J., Webster, C., & Kahlmeter, G. (2018). Antimicrobial susceptibility testing of colistin - evaluation of seven commercial MIC products against standard broth microdilution for Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, and Acinetobacter spp. Clin Microbiol Infect, 24(8), 865-870. doi:10.1016/j.cmi.2017.11.020
Nation, R. L., Garonzik, S. M., Thamlikitkul, V., Giamarellos-Bourboulis, E. J., Forrest, A., Paterson, D. L., et al. (2017). Dosing guidance for intravenous colistin in critically-ill patients. Clin Infect Dis, 64(5), 565-571. doi:10.1093/cid/ciw839
Qureshi, Z. A., Hittle, L. E., O'Hara, J. A., Rivera, J. I., Syed, A., Shields, R. K., et al. (2015). Colistin-resistant Acinetobacter baumannii: beyond carbapenem resistance. Clin Infect Dis, 60(9), 1295-1303. doi:10.1093/cid/civ048
Simner, P. J., Bergman, Y., Trejo, M., Roberts, A. A., Marayan, R., Tekle, T., et al. (2019). Two-site evaluation of the colistin broth disk elution test to determine colistin in vitro activity against Gram-negative bacilli. J Clin Microbiol, 57(2). doi:10.1128/jcm.01163-18
Tan, T. Y., & Ng, L. S. (2006). Comparison of three standardized disc susceptibility testing methods for colistin. J Antimicrob Chemother, 58(4), 864-867. doi:10.1093/jac/dkl330
Viehman, J. A., Nguyen, M. H., & Doi, Y. (2014). Treatment options for carbapenem-resistant and extensively drug-resistant Acinetobacter baumannii infections. Drugs, 74(12), 1315-1333. doi:10.1007/s40265-014-0267-8
Downloads
Published
2022-02-02
Issue
Section
Research Article
