Effect of Supplementary Diet Mixed with Probiotics on Colony Population of Apis mellifera Linnaeus, 1758
Abstract
This study aimed to investigate the effect of supplementary diets mixed with four species of the probiotics, Lactobacillus casei TISTR 390, L. plantarum TISTR 541, L. curvatus TISTR 938 and L. acidophilus TISTR 2365 on colony population of the honey bee, Apis mellifera L. Number of eggs, larvae, pupae and adults in every week and spent the time for 12 weeks (30 colonies divided into 6 treated groups with consist of 5 colonies) by the standard method (Burgett and Burikam, 1985). The results showed that the treated groups of bees which were fed by the food mixed with L. curvatus (96.60±45.68 %), followed by colonies provided by food mixed with L. acidophilus (74.37±16.38 %), L. plantarum (73.14±12.57 %), L. casei (71.54±7.30 %), without probiotics (69.39±11.66 %) and natural foods (58.40±14.41 %) had been much involved in the increase of the rate of population insignificantly and respectively [F (5, 24) = 2.305, p = 0.076]. The research indicates that all the four species of probiotics are involved in the increase of rate of bee population in the colonies. It is considered to be progressively developed as a mixture of supplementary diet for commercial bee keeping. Keywords : supplementary diet ; probiotics ; colony population ; Apis melliferaReferences
Amiri, E., Strand, M.K., Rueppell, O., & Tarpy, D.R. (2017). Queen quality and the impact of honey bee diseases on queen health: Potential for interactions between two major threats to colony health. Insects, 8(2), 48; doi:10.3390/insects8020048
Anderson, K.E., Sheehan, T.H., Eckholm, B.J., Mott B.M., & DeGrandi-Hoffman G. (2011). An emerging paradigm of colony health: microbial balance of the honey bee and hive (Apis mellifera). Insectes Sociaux, 58, 431. https://doi.org/10.1007/s00040-011-0194-6
Barron, A.B. (2015). Death of the bee hive: Understanding the failure of an insect society. Current Opinion in Insect Science, 10, 45-50. https://doi.org/10.1016/j.cois.2015.04.004
Bee health. (2019). What is causing the decline of honey bee populations?. Retrieved May 18, 2020, from https://bee-health.extension.org/what-is-causing-the-decline-of-honey-bee-populations/
Burgett, D.M., & Burikam, I. (1985). Number of adult honey bees (Hymenoptera: Apidae) occupying a comb: A standard for estimating colony populations. Journal of Economic Entomology, 78, 1154-1156.
Chaiyasut, C. (2011). Probiotics: Microorganisms for Life. Bangkok: Infinity color Printing Co.,Ltd. (in Thai)
Chandran, H., & Raghavan K.T. (2018). Probiotic potency of Lactobacillus plantarum KX519413 and KX519414 isolated from honey bee gut. FEMS Microbiology Letters, 365(4), 1-8.
Chantawannakul, P., & Maitip, J. (2017). Novel Technologies for beekeeping and bee product industries. research results into utilization. NRCT Newsletter, 12(77), 5-7. (in Thai)
Chantawannakul, P., Sanghiran Lee, V., Nimmanpipug, P., & Krongdang, S. (2018). Bee, Pesticide, The Impact on Ecosystem and Human Health. Chiangmai: MaxxPRINTINGTM (in Thai)
Coeuret, V., Gueguen M., & Vernoux J.P. (2004). Numbers and strains of lactobacilli in some probiotic products. International Journal of Food Microbiology, 97, 147-56.
Davis, C., & Ward, W. (2003). The control of chalkbrood disease with natural products. Journal of Apicultural Research., 42(3bvg), 1-23.
De Man J.C., Rogosa M., & Sharpe, M.E. (1960). A medium for the cultivation of lactobacilli. Journal of Applied Bacteriology, 23, 130-135.
Evans, J., & Lopez, D.L. (2004). Bacterial probiotics induce an immune response in the honey bee (Hymenoptera: Apidae). Journal of Economic Entomology, 97(3), 752-756.
Fine JD, Shpigler HY, Ray AM, Beach NJ, Sankey AL, Cash-Ahmed A, Huang, ZY, Astrauskaite, L., Chao, R., Zhao, H., & Robinson, G.E. (2018). Quantifying the effects of pollen nutrition on honey bee queen egg laying with a new laboratory system. PLoS ONE, 13(9): e0203444. https://doi.org/10.1371/journa.pone. 0203444
Flynn, S., van Sinderen, D., Thornton, G.M., Holo, H., Nes, I.F., & Collins, J.K. (2002). Characterization of the genetic locus responsible for the production of ABP-118, a novel bacteriocin produced by the probiotic bacterium Lactobacillus salivarius subsp. salivarius UCC118. Microbiology, 148(4), 973–984.
Gojmerac, W.L. (1980). Bee, beekeeping, honey and pollination. Westport, Conn.: AVI Publishing.
Mathialagan, M., Johnson Thangaraj Edward, Y.S., David, P.M.M., Senthilkumar, M., Srinivasan, M.R., & Mohankumar, S. (2018). Isolation, characterization and identification of probiotic lactic acid bacteria (LAB) from honey bees. International Journal of Current Microbiology and Applied Sciences, 7(4), 894-906.
Meidong, R., Khotchanalekha, K., Doolgindachbaporn, S., Nagasawa, T., Nakao, M., Sakai, K., & Tongpim, S. (2017). Evaluation of probiotic Bacillus aerius B81e isolated from healthy hybrid catfish on growth, disease resistance and innate immunity of Pla-mong Pangasius bocourti. Fish and Shellfish Immunology, 73, 1-10. (in Thai)
Syromyatnikov, M., Isuwa, M.M., Savinkova, O.V., Derevshchikova, M.I., & Popov, V.N. (2020). The effect of pesticides on the microbiome of animals. Agriculture, 10(79), 1-14.
Mountzouris, K., Tsitrsikos, P., Palamidi, I., Arvaniti, A., Mohnl, M., Schatzmayr, G., & Fegeros, K. (2010). Effects of probiotic inclusion levels in broiler nutrition on growth performance, nutrient digestibility, plasma immunoglobulins, and cecal microflora composition. Poultry Science, 89(1), 58-67.
Patruica, S., & Hutu, I. (2013). Economic benefits of using prebiotic and probiotic products as supplements in stimulation feeds administered to bee colonies. Turkish Journal of Veterinary and Animal Sciences, 37, 259-263.
Pattabhiramaiah, M., Reddy, M.S., & Brueckner, D. (2012). Detection of novel probiotic bacterium Lactobacillus spp. in the workers of Indian honeybee, Apis cerana indica. International Journal of Environmental Sciences, 2(3), 1135-1143.
Ptaszynska, A.A., Borsuk, G., Zdybicka-Barabas, A., Cytryriska, M., & Malek, W. (2016). Are commercial probiotics and prebiotics effective in the treatment and prevention of honeybee nosemosis C. Parasitology Research, 115(1), 397-406.
Rangel, J., & Fisher, A. II. (2019). Factors affecting the reproductive health of honey bee (Apis mellifera) drones-a review. Apidologie, 50, 759–778.
Sammataro, D. (2013, August 13). Beneficial microflora in honey bee colonies [Video file]. Retrieved June 23, 2019, from https://www.youtube.com/watch?v=NI9Z73UAX-g
Szymas, B., Langowska, A., & Kazimierczak-Baryczko, M. (2012). Histological structure of the midgut of honey bees (Apis mellifera L.) fed pollen substitutes fortified with probiotics. Journal of Apicultural Science, 56(1), 5-12.
Anderson, K.E., Sheehan, T.H., Eckholm, B.J., Mott B.M., & DeGrandi-Hoffman G. (2011). An emerging paradigm of colony health: microbial balance of the honey bee and hive (Apis mellifera). Insectes Sociaux, 58, 431. https://doi.org/10.1007/s00040-011-0194-6
Barron, A.B. (2015). Death of the bee hive: Understanding the failure of an insect society. Current Opinion in Insect Science, 10, 45-50. https://doi.org/10.1016/j.cois.2015.04.004
Bee health. (2019). What is causing the decline of honey bee populations?. Retrieved May 18, 2020, from https://bee-health.extension.org/what-is-causing-the-decline-of-honey-bee-populations/
Burgett, D.M., & Burikam, I. (1985). Number of adult honey bees (Hymenoptera: Apidae) occupying a comb: A standard for estimating colony populations. Journal of Economic Entomology, 78, 1154-1156.
Chaiyasut, C. (2011). Probiotics: Microorganisms for Life. Bangkok: Infinity color Printing Co.,Ltd. (in Thai)
Chandran, H., & Raghavan K.T. (2018). Probiotic potency of Lactobacillus plantarum KX519413 and KX519414 isolated from honey bee gut. FEMS Microbiology Letters, 365(4), 1-8.
Chantawannakul, P., & Maitip, J. (2017). Novel Technologies for beekeeping and bee product industries. research results into utilization. NRCT Newsletter, 12(77), 5-7. (in Thai)
Chantawannakul, P., Sanghiran Lee, V., Nimmanpipug, P., & Krongdang, S. (2018). Bee, Pesticide, The Impact on Ecosystem and Human Health. Chiangmai: MaxxPRINTINGTM (in Thai)
Coeuret, V., Gueguen M., & Vernoux J.P. (2004). Numbers and strains of lactobacilli in some probiotic products. International Journal of Food Microbiology, 97, 147-56.
Davis, C., & Ward, W. (2003). The control of chalkbrood disease with natural products. Journal of Apicultural Research., 42(3bvg), 1-23.
De Man J.C., Rogosa M., & Sharpe, M.E. (1960). A medium for the cultivation of lactobacilli. Journal of Applied Bacteriology, 23, 130-135.
Evans, J., & Lopez, D.L. (2004). Bacterial probiotics induce an immune response in the honey bee (Hymenoptera: Apidae). Journal of Economic Entomology, 97(3), 752-756.
Fine JD, Shpigler HY, Ray AM, Beach NJ, Sankey AL, Cash-Ahmed A, Huang, ZY, Astrauskaite, L., Chao, R., Zhao, H., & Robinson, G.E. (2018). Quantifying the effects of pollen nutrition on honey bee queen egg laying with a new laboratory system. PLoS ONE, 13(9): e0203444. https://doi.org/10.1371/journa.pone. 0203444
Flynn, S., van Sinderen, D., Thornton, G.M., Holo, H., Nes, I.F., & Collins, J.K. (2002). Characterization of the genetic locus responsible for the production of ABP-118, a novel bacteriocin produced by the probiotic bacterium Lactobacillus salivarius subsp. salivarius UCC118. Microbiology, 148(4), 973–984.
Gojmerac, W.L. (1980). Bee, beekeeping, honey and pollination. Westport, Conn.: AVI Publishing.
Mathialagan, M., Johnson Thangaraj Edward, Y.S., David, P.M.M., Senthilkumar, M., Srinivasan, M.R., & Mohankumar, S. (2018). Isolation, characterization and identification of probiotic lactic acid bacteria (LAB) from honey bees. International Journal of Current Microbiology and Applied Sciences, 7(4), 894-906.
Meidong, R., Khotchanalekha, K., Doolgindachbaporn, S., Nagasawa, T., Nakao, M., Sakai, K., & Tongpim, S. (2017). Evaluation of probiotic Bacillus aerius B81e isolated from healthy hybrid catfish on growth, disease resistance and innate immunity of Pla-mong Pangasius bocourti. Fish and Shellfish Immunology, 73, 1-10. (in Thai)
Syromyatnikov, M., Isuwa, M.M., Savinkova, O.V., Derevshchikova, M.I., & Popov, V.N. (2020). The effect of pesticides on the microbiome of animals. Agriculture, 10(79), 1-14.
Mountzouris, K., Tsitrsikos, P., Palamidi, I., Arvaniti, A., Mohnl, M., Schatzmayr, G., & Fegeros, K. (2010). Effects of probiotic inclusion levels in broiler nutrition on growth performance, nutrient digestibility, plasma immunoglobulins, and cecal microflora composition. Poultry Science, 89(1), 58-67.
Patruica, S., & Hutu, I. (2013). Economic benefits of using prebiotic and probiotic products as supplements in stimulation feeds administered to bee colonies. Turkish Journal of Veterinary and Animal Sciences, 37, 259-263.
Pattabhiramaiah, M., Reddy, M.S., & Brueckner, D. (2012). Detection of novel probiotic bacterium Lactobacillus spp. in the workers of Indian honeybee, Apis cerana indica. International Journal of Environmental Sciences, 2(3), 1135-1143.
Ptaszynska, A.A., Borsuk, G., Zdybicka-Barabas, A., Cytryriska, M., & Malek, W. (2016). Are commercial probiotics and prebiotics effective in the treatment and prevention of honeybee nosemosis C. Parasitology Research, 115(1), 397-406.
Rangel, J., & Fisher, A. II. (2019). Factors affecting the reproductive health of honey bee (Apis mellifera) drones-a review. Apidologie, 50, 759–778.
Sammataro, D. (2013, August 13). Beneficial microflora in honey bee colonies [Video file]. Retrieved June 23, 2019, from https://www.youtube.com/watch?v=NI9Z73UAX-g
Szymas, B., Langowska, A., & Kazimierczak-Baryczko, M. (2012). Histological structure of the midgut of honey bees (Apis mellifera L.) fed pollen substitutes fortified with probiotics. Journal of Apicultural Science, 56(1), 5-12.
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2021-09-09
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