Systematics in Family Nymphaeaceae Based on Chloroplast DNA
Abstract
บทคัดย่อ พืชในวงศ์บัวสาย (Nymphaeaceae) มีการกระจายพันธุ์อยู่เกือบทั่วโลกทั้งในเขตร้อนและเขตอบอุ่น เป็นพืชที่ได้รับความนิยมในการปลูกเป็นไม้ประดับ พืชในวงศ์บัวสาย หลายชนิดมีลักษณะของสีกลีบดอก รูปร่างดอก หรือใบที่คล้ายคลึงกัน ทำให้ประสบปัญหาในการจัดจำแนกโดยใช้ลักษณะทางสัณฐานวิทยาเพียงอย่างเดียว ดังนั้นเพื่อให้สามารถจัดจำแนกได้ถูกต้องตามชนิดพันธุ์จึงต้องอาศัยข้อมูลทางชีวโมเลกุลเพื่อวิเคราะห์ความสัมพันธ์ทางวิวัฒนาการด้วยวิธีการวิเคราะห์แบบ Maximum parsimony โดยในการศึกษาครั้งนี้ใช้ข้อมูลของลำดับดีเอ็นเอบริเวณยีน trnK-matK และบริเวณระหว่างยีน trnL-F ในคลอโรพลาสต์ พบว่าชิ้นส่วนดีเอ็นเอที่เพิ่มปริมาณได้มีขนาด 1180-1209 คู่เบส และ 991-1095 คู่เบส ตามลำดับ ผลการศึกษาพบว่าReferences
คุณหญิงสุชาดา ศรีเพ็ญ, วีรญา บุญเตี้ย และวิสาขา เพียรสุภาพ. (2550). การจำแนกพันธุ์บัว. The Proceeding of IWGS Annual Symposium,
181–189.
Borsch, T., Lohne, C., Mbaye, M.S. and Wiersema J.H. (2011). Towards a complete species tree of Nymphaea: shedding further light on subgenus Brachyceras and its relationships to the Australian water-lilies. Telopea, 13(1-2), 193-217.
Conard, H.S. (1905). The Waterlilies: A Monograph of the Genus Nymphaea. Washington: Carnegie Institution.
Doyle, J. and Doyle, J. (1987). A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemical Bulletin, 19, 11-15.
Hilu, K.W., Borsch, T., Muller, K., Soltis D.E., Soltis, P.S. et al. (2003). Angiosperm Phylogeny Based on matK Sequence Information. American Journal of Botany, 90(12), 1758–1776.
Ito, M. (1987). Phylogenetic Systematics of the Nymphaeales. The botanical magazine, Tokyo, 100, 17-35
Kuo, L.Y., Li, F.W., Chiou, W.L. and Wang, C.N. (2011). First insights into fern matK phylogeny. Molecular Phylogenetics and Evolution, 59, 556–566
Les, D.H., Schneider, E.L., Padgett, D.J., Soltis, P.S., Soltis, D.E. and Zanis, M. (1999). Phylogeny, classification and floral evolution of waterlilies (Nymphaeaceae: Nymphaeales): a synthesis of non-molecular, rbcL, matK, and 18s rDNA data. Systematic Botany, 24, 28–46.
Lohne, C., Borsch, T. and Wiersema, J.H. (2007). Phylogenetic analysis of Nymphaeales using fast-evolving and noncoding chloroplast markers. Botanical Journal of the Linnean Society, 154, 141–163.
Lohne, C., Borsch, T., Jacobs, S.W.L., Hellquist, C.B. and Wiersema, J.H. (2008). Nuclear and plastid DNA sequences reveal complex reticulate patterns in Australian water-lilies (Nymphaea subgenus Anecphya, Nymphaeaceae). Australian Systematic Botany, 21, 229-250.
Nicholas, K.B. and Nicholas, H.J.B. (1997). GeneDoc: a tool for editing and annotating multiple sequence alignment. Retrieved August 20, 2013, from www.psc.edu/biomed/genedoc.
Padgett, D.J., Les, D.H. and Crow, G.E. (1999). Phylogenetic relationships in Nuphar (Nymphaeaceae): Evidence from morphology, chloroplast DNA, and nuclear ribosomal DNA. Bridgewater State College, Bridgewater, USA.
Petit, R.J., Demesure, B., and Dumolin-Lapègue, S. (1996). CpDNA and plant mtDNA primers. In Molecular tools for screening biodiversity: Plants and Animals. A Karp, PG Isaac, D Ingram eds, Chapman & Hall.
Podoplelova, Y. and Ryzhakov, G. (2005). Phylogenetic analysis of the order Nymphaeales based on the nucleotide sequences of the chloroplast ITS2-4 region. Plant Science,169, 606–611.
Slocum, D. P. (2005). Waterlilies and lotuses: species, cultivars, and new hybrids. Timber press. 260 pages.
Taberlet, P., Gielly, L., Pautou, G. and Bouvet, J. (1991). Universal primers for amplification of three non-coding regions of Chloroplast DNA. Plant Molecular Biology, 17, 1105-1109.
Tamura, K., Peterson, D., Peterson, N., Stecher, G., Nei, M., and Kumar, S. (2011). MEGA5: Molecular Evolutionary Genetics Analysis using Maximum Likelihood, Evolution Distance, and Maximum Parsimony Methods. Molecular Biology and Evolution, 28, 2731–2739.
Thompson, J.D., Gibson, T.J., Plewniak, F., Jeanmougin, F. and Higgins, D.G. (1997). The ClustalX windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tool. Nucleic Acids Research, 24, 4876-4882.
181–189.
Borsch, T., Lohne, C., Mbaye, M.S. and Wiersema J.H. (2011). Towards a complete species tree of Nymphaea: shedding further light on subgenus Brachyceras and its relationships to the Australian water-lilies. Telopea, 13(1-2), 193-217.
Conard, H.S. (1905). The Waterlilies: A Monograph of the Genus Nymphaea. Washington: Carnegie Institution.
Doyle, J. and Doyle, J. (1987). A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemical Bulletin, 19, 11-15.
Hilu, K.W., Borsch, T., Muller, K., Soltis D.E., Soltis, P.S. et al. (2003). Angiosperm Phylogeny Based on matK Sequence Information. American Journal of Botany, 90(12), 1758–1776.
Ito, M. (1987). Phylogenetic Systematics of the Nymphaeales. The botanical magazine, Tokyo, 100, 17-35
Kuo, L.Y., Li, F.W., Chiou, W.L. and Wang, C.N. (2011). First insights into fern matK phylogeny. Molecular Phylogenetics and Evolution, 59, 556–566
Les, D.H., Schneider, E.L., Padgett, D.J., Soltis, P.S., Soltis, D.E. and Zanis, M. (1999). Phylogeny, classification and floral evolution of waterlilies (Nymphaeaceae: Nymphaeales): a synthesis of non-molecular, rbcL, matK, and 18s rDNA data. Systematic Botany, 24, 28–46.
Lohne, C., Borsch, T. and Wiersema, J.H. (2007). Phylogenetic analysis of Nymphaeales using fast-evolving and noncoding chloroplast markers. Botanical Journal of the Linnean Society, 154, 141–163.
Lohne, C., Borsch, T., Jacobs, S.W.L., Hellquist, C.B. and Wiersema, J.H. (2008). Nuclear and plastid DNA sequences reveal complex reticulate patterns in Australian water-lilies (Nymphaea subgenus Anecphya, Nymphaeaceae). Australian Systematic Botany, 21, 229-250.
Nicholas, K.B. and Nicholas, H.J.B. (1997). GeneDoc: a tool for editing and annotating multiple sequence alignment. Retrieved August 20, 2013, from www.psc.edu/biomed/genedoc.
Padgett, D.J., Les, D.H. and Crow, G.E. (1999). Phylogenetic relationships in Nuphar (Nymphaeaceae): Evidence from morphology, chloroplast DNA, and nuclear ribosomal DNA. Bridgewater State College, Bridgewater, USA.
Petit, R.J., Demesure, B., and Dumolin-Lapègue, S. (1996). CpDNA and plant mtDNA primers. In Molecular tools for screening biodiversity: Plants and Animals. A Karp, PG Isaac, D Ingram eds, Chapman & Hall.
Podoplelova, Y. and Ryzhakov, G. (2005). Phylogenetic analysis of the order Nymphaeales based on the nucleotide sequences of the chloroplast ITS2-4 region. Plant Science,169, 606–611.
Slocum, D. P. (2005). Waterlilies and lotuses: species, cultivars, and new hybrids. Timber press. 260 pages.
Taberlet, P., Gielly, L., Pautou, G. and Bouvet, J. (1991). Universal primers for amplification of three non-coding regions of Chloroplast DNA. Plant Molecular Biology, 17, 1105-1109.
Tamura, K., Peterson, D., Peterson, N., Stecher, G., Nei, M., and Kumar, S. (2011). MEGA5: Molecular Evolutionary Genetics Analysis using Maximum Likelihood, Evolution Distance, and Maximum Parsimony Methods. Molecular Biology and Evolution, 28, 2731–2739.
Thompson, J.D., Gibson, T.J., Plewniak, F., Jeanmougin, F. and Higgins, D.G. (1997). The ClustalX windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tool. Nucleic Acids Research, 24, 4876-4882.
