Chemical Constituents and Biological Activities of Unripe Fruits Seeds of Annona squamosa
Abstract
Unripe fruits of Annona squamosa Linn. were unripe before mature, and there were a few useful. This research aimed to study chemical constituents, anti-lung cancer and anti-head lice in vitro of extract of unripe fruit seeds of A. squamosa Linn. The extract was successively extracted with hexane. The study of chemical constituents was measured by GC-MS. Antitumor activity toward human small cell lung carcinoma (NCI-H187) by using Rasazurin microplate assay (REMA) method and positive controls were ellipticine and doxorubicin. Anti-head lice effect in vitro were tested with 10% w/w hexane extract in coconut oil, 10% w/w hexane extract in soy bean oil, seed oil of unripe fruits in coconut oil, and seed oil of unripe fruits in soy bean oil with 1:2 of rate. The seed oil of ripe fruit in coconut oil and soy bean oil were used as positive controls, but coconut oil and soy bean oil were used as negative controls. The statistics of this research were percentage, mean, standard deviation and comparison with One-way ANOVA by Ducan test at 0.05 significant. The hexane extract had major constituent as oleic acid which showed the highest quantitative value of 50.62 percentage, following ethyl oleate, n-hexadecanoic acid, ethyl linoleate, ethyl exadecanoate, toluene, ethyl octadecanoate, 2,3-dihydroxypropyl elaidate and (E)- methyl- 9-octadecenoate, respectively, by GC-MS analysis. At concentration of 50 µg/ml, hexane extract showed high activity against NCI-H187 cell line which the value of 76.74 percentage. The killing time (KT) of head lice effect of 10% w/w hexane extract in coconut oil, seeds oil of unripe fruits in coconut oil, and seed oil of unripe fruits in soy bean oil with 1:2 of rate showed highest activities of 46.7±2.9 53.3±2.9and 63.3±7.6 minute, respectively. They were not significantly at 0.05, but they were differently with other tests with significant of 0.05. Keywords : Unripe fruits of Annona squamosa, biological activities, chemical constituentsReferences
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Precursors from the seeds of Annona squamosa. Phytochemistry Letters. 272–76.
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IJRPBS., 2(4). 1404-1412.
Sansuk, M. (2016). Nouyhnapunmaiphaykaeykasead 2. Bangkok. Ypsee group. (in Thai)
Siriudompas, S. (2016). Houlaeloan. Bangkok. Rangsit University. (in Thai)
Smitinand, T. (2016). Thai plant names (botanical names-vernacular names) revised edition. Bangkok. The forest
Herbarium, Royal Forest Department. (in Thai)
for the assessment of mammalian cell cytotoxicity. Eur J Biochem., 267, 5421-6.
Chen, Y., Chen, J., Wang, Y., Xu S., Li. X. (2012a). Six cytotoxic annonaceous acetogenins from Annona squamosa
seeds. Food Chem., 135, 960–966.
Chen, Y. Xu, S. Chen, J. Wang, Y. Xu, H. Fan, N. Li X. (2012b). Anti-tumor activity of Annona squamosa
seeds extract containing annonaceous acetogenin compounds. J Ethnopharmacol., 142, 462–466.
Intaranongpai, J., Chavasiri W. and Gritsanapan. W. (2006). AntiI-head lice effect of Annona squamosa seeds.
Southeast Asian J Trop Med Public Health., 37(3), 532-535.
Jayendra, and Kumar Y. (2 0 1 3). New compound 6,7-dimethoxy-2-methylisoquinolinium from Indian medicinal
plant Annona squamosa L. ijcas., 4,161-168.
Masruri, Sharma, M., Warsito, and Adi, P. (2012). Renewable oil extracted from Indonesian Srikaya’s (Annona
squamosa sp.) seed: another potent source for biodiesel. J. Pure App. Chem. Res., 1 (1), 51‐57.
Naykasead. (2015). Nouyhnataipraykupsuppakuldeedee. Thairath. 12 October, 2015. (in Thai)
Ndob, I.B. ba, Champy, P., Gleye, C., Lewin, G. and Akendengue, B. (2009). Annonaceous acetogenins:
Precursors from the seeds of Annona squamosa. Phytochemistry Letters. 272–76.
Pandey, N. and Barve D. (2011). Phytochemical and Pharmacological Review on Annona squamosa Linn.
IJRPBS., 2(4). 1404-1412.
Sansuk, M. (2016). Nouyhnapunmaiphaykaeykasead 2. Bangkok. Ypsee group. (in Thai)
Siriudompas, S. (2016). Houlaeloan. Bangkok. Rangsit University. (in Thai)
Smitinand, T. (2016). Thai plant names (botanical names-vernacular names) revised edition. Bangkok. The forest
Herbarium, Royal Forest Department. (in Thai)
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2017-11-20
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