A Comparative Study on Antioxidant and Nitric Oxide-Inducing Activity of Some Watermelon Cultivars Grown in Thailand
Abstract
Watermelon (Citrullus lanatus) is a widely consumed fruit. It is known that the cultivar and parts of fruit affect biological activities of watermelon. Thus, the aim of this study was to comparatively study on antioxidant and nitric oxide-inducing activities of different parts (outer skin, epicarp, mesocarp and seeds) of five watermelon cultivars grown in Thailand (Kinnaree, Torpedo, Yaya, Runrun and King orange). The antioxidant activity was measured by DPPH radical scavenging activity assay. The inducing effect on nitric oxide (NO) production was determined in human vein endothelial cells (EA.hy 926). It was found that the extract of all cultivars exhibited DPPH radical scavenging activity and induced NO production. The outer skin of Torpedo cultivar showed the highest antioxidant and NO-inducing activities. Furthermore, the results demonstrate that the cultivars and fruit sampling area influence on antioxidant and NO-inducing activity of watermelon. The obtained data is probably used for promotion on consumption of watermelon and in the development of food supplements from these watermelon cultivars. Keywords : watermelon, antioxidant activity, nitric oxide-inducing activity, endothelial cellReferences
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Rimando, A.M. and Perkins-Veazie, P.M. (2005). Determination of citrulline in watermelon rind. Journal of Chromatography A, 1078, 196-200.
Srisook, K., Salee, P., Charoensuk, Y., Srisook, E. (2010). In vitro anti-oxidant and anti-tyrosinase activities of the rhizome extracts from Amomum biflorum Jack. Thai Journal of Botany. 2, 143-150.
Srisook, K., Srisook, E., Nachaiyo, W., Chan-In, M., Thongbai, J., Wongyoo, K., et al. (2015). Bioassay-guided isolation and mechanistic action of anti-inflammatory agents from Clerodendrum inerme leaves. Journal of Ethnopharmacology, 165, 94-102.
Tlili, I, Hdider, C., Lenucci, M.S., Riadh, I., Jebari, H., Dalessandro. G. (2011). Bioactive compounds and antioxidant activities of different watermelon (Citrullus lanatus (Thunb.) Mansfeld) cultivars as affected by fruit sampling area. Journal of Food Composition and Analysis, 24, 307–314.
Tlili, I., Hdider, C., Ilahy, R., Jebari. H. (2010). Phytochemical composition and antioxidant activity of selected watermelon varieties grown in Tunisia. The African Journal of Plant Science and Biotechnology, 4,
68-71.
Vanhoutte, P.M, Shimokawa, H., Tang, E.H., Feletou, M. (2009). Endothelial dysfunction and vascular disease.
Acta physiologica (Oxf), 196, 193-222.
Choo, W.S., Shin, W.Y. (2012). Ascorbic acid, lycopene and antioxidant activities of red-fleshed and yellow-fleshed watermelons. Advances in Applied Science Research, 3, 2779-2784.
Davis, A.R., Webber, C.L. and Fish, W.W., King, W.S., Perkins-Veazie, P. (2011). L-Citrulline levels in watermelon cultigens tested in two environment. HortScience, 46, 1572-1575.
Flam, B.R., Eichler, D.C., Solomonson, L.P. (2007). Endothelial nitric oxide production is tightly coupled to the citrulline–NO cycle. Nitric Oxide, 17, 115–121.
Forstermann, U. and Sessa, W. (2012). Nitric oxide synthases: regulation and function. European Heart Journal, 33, 829–837.
Kim, C.H., Park, M.K., Kim, S.K., and Cho, Y.H. (2014). Antioxidant capacity and anti-inflammatory activity of lycopene in watermelon. International Journal of Food Science and Technology, 49, 2083–2091
Loypimai, P., Pasakul, T., Mongkolthai, R. (2011). Comparisons of antioxidant activities and total phenolic content of fruit peels. Agricultural Science Journal. 42, 385-388.
Oseni, O. A. and Okoye, V. I. (2013). Studies of phytochemical and antioxidant properties of the fruit of watermelon (Citrullus lanatus). (Thunb.). Journal of Pharmaceutical and Biomedical Sciences, 27,
508-514.
Ratanaopa, S. and Sirisomboon, P. (2013). Change in lycopene and soluble solids content of watermelon (Kinnaree variety) at different maturity. Proceedings of the 14th TSAE National Conference, pp. 157-158.
Rimando, A.M. and Perkins-Veazie, P.M. (2005). Determination of citrulline in watermelon rind. Journal of Chromatography A, 1078, 196-200.
Srisook, K., Salee, P., Charoensuk, Y., Srisook, E. (2010). In vitro anti-oxidant and anti-tyrosinase activities of the rhizome extracts from Amomum biflorum Jack. Thai Journal of Botany. 2, 143-150.
Srisook, K., Srisook, E., Nachaiyo, W., Chan-In, M., Thongbai, J., Wongyoo, K., et al. (2015). Bioassay-guided isolation and mechanistic action of anti-inflammatory agents from Clerodendrum inerme leaves. Journal of Ethnopharmacology, 165, 94-102.
Tlili, I, Hdider, C., Lenucci, M.S., Riadh, I., Jebari, H., Dalessandro. G. (2011). Bioactive compounds and antioxidant activities of different watermelon (Citrullus lanatus (Thunb.) Mansfeld) cultivars as affected by fruit sampling area. Journal of Food Composition and Analysis, 24, 307–314.
Tlili, I., Hdider, C., Ilahy, R., Jebari. H. (2010). Phytochemical composition and antioxidant activity of selected watermelon varieties grown in Tunisia. The African Journal of Plant Science and Biotechnology, 4,
68-71.
Vanhoutte, P.M, Shimokawa, H., Tang, E.H., Feletou, M. (2009). Endothelial dysfunction and vascular disease.
Acta physiologica (Oxf), 196, 193-222.
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Published
2017-06-30
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บทความวิจัยจากการประชุมวิชาการระดับชาติ"วิทยาศาสตร์วิจัย"ครั้งที่ 9
