Efficacy of Antagonistic Yeasts on Biological Control of Gray Mold Rot of Post-Harvest Grape Caused by Botrytis cinerea
Abstract
Gray mold rot is a destructive disease to postharvest grape. Biological control is an alternative way to reduce the problems from using synthetic fungicides. This research aimed to study the effectiveness of antagonistic yeasts in controlling gray mold rot disease caused by Botrytis cinerea in postharvest grape. Twenty isolates of yeast were isolated from peel of grave cv. Cardinal. Among them, yeast isolate B-603 had significantly highest inhibitory effect 64.9±3.2% to B. cinerea in dual culture on PDA. Yeast isolate B-603 was tested for controlling B. cinerea in postharvest grape cv. Cardinal. It was found that isolate B-603 at 1×106, 1×107 and 1×108 cells/ml could completely control (100%) the gray mold disease compared to the control. For the numbers of antagonistic yeast and pathogen on the surface of grape berries, yeast isolate B-603 was increased their populations when it was applied at 1×106 and 1×107 cells/ml. However, the numbers of antagonistic yeast were decreased when using the concentration at 1×108 cells/ml. In addition, B. cinerea was not found on grape berries applying with all concentrations of antagonistic yeast isolate B-603. Keywords: gray mold disease, antagonistic yeast, Botrytis cinerea, grapeReferences
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italicum by Kloeckera apiculate, Biological Control, 67, 157–162.
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(2012). Biocontrol of Botrytis cinerea in table grapes by non-pathogenic indigenous Saccharomyces
cerevisiae yeasts isolated from viticultural environments in Argentina. Postharvest Biology and Technology, 64(1), 40-48.
Pantelides, L. S., Christou, O., Tsolakidon, M-D., Tsaltas, D. and Ioannou, N. (2015). Isolation, identification and in
vivo screening of grapevine yeasts for the control of black aspergilli on grape. Biological Control,
88, 46-53.
Qin, X., Xiao, H., Xue, C., Yu, Z., Yang, R., Cai, Z.and Si, L. (2015) Biocontrol of gray mold in grapes with the yeast
Hanseniaspora uvarum alone and in combination with salicylic acid or sodium bicarbonate. Postharvest Biology and Technology, 100, 160-167.
Sansone, G., Rezza, I., Fernandez, G., Calvente, V., Benuzzi, D., and Sanz, M.I. (2011). Inhibitors of
polygalacturonase and laccase of Botrytis cinerea and their application to the control of this fungus. International Biodeterioration and Biodegradation, 65, 243-247.
Senthil, R., Prabakar, K., Rajendran, I. and Karthikeyan, G. (2011). Efficacy of different biological
control agents against major postharvest pathogens of grapes under room temperature storage conditions. Phytopathologia Mediterranean, 50, 55-65.
Spadaro, D. and Droby, S. 2016. Development of biocontrol products for postharvest diseases of fruit: the
importance of elucidating the mechanisms of action of yeast antagonists. Trends in Food Science &
Technology, 47(39-49)
Vero, S., Garmendia, G., Gonzale, M.B., Bentancur, O., and Wisniewski, M. (2013). Evaluation of yeasts obtained
from Antarctic soil samples as biocontrol agents for the management of postharvest diseases of apple.
FEMS Yeast Research, 13, 189-199.
Wang, Y., Yu, T., Xia, J., Yu, D., Wang, J. and Zheng, X. (2010). Biocontrol of postharvest gray mold of cherry
tomatoes with the marine yeast Rhodosporidium paludigenum. Biological Control, 53, 178-182.
Zhang, D., Spadaro, D., Garibaldi, A., and Gullino, L.M. (2011). Potential biocontrol activity of a Pichia
guilliermondii against grey mold of apple and its possible modes of action. Biological Control, 57,
193-201.
Phytopathological Society. Minnesota, St. Paul.
Calvo-Garrido, C., Elmer, P.A.G., Vinas, I., Usall, J., Bartra, E., and Teixido, N. (2013). Biological control of botrytis
bunch rot in organic wine grape with the yeast antagonist Candida sake CPA-1. Plant Pathology,
62(3), 510-519.
Jaconmetti, M.A., Wratten, S.D., and Walter, M. (2010). Alternative to synthetic fungicides for Botrytis cinerea
management in vineyard. Australian Journal of Grape and Wine Research, 16, 154-172.
Kwasiborski, A., Bajji, M., Renaut, J., Delaplace, P., and Jijakli, M.H. (2014). Identification of metabolic pathways
expressed by Pichia anomala Kh6 in the presence of the pathogen Botrytis cinerea on apple: new
possible targets for biocontrol improvement. PLoS One, 9(3), e91434.
Leroux P. (2007) Chemical Control of Botrytis and its Resistance to Chemical Fungicides. In: Elad Y., Williamson
B., Tudzynski P., Delen N. (eds) Botrytis: Biology, Pathology and Control. Springer, Dordrecht.
Liu, H.M., Guo, J.H., Luo, L., Liu, P., Wang, B.Q., Cheng, Y.J., Deng, B.X., and Long, C.A. (2010). Improvement of
Hanseniaspora uvarum biocontrol activity against gray mold by the addition of ammonium molybdate
and the possible mechanisms involved. Crop Protection, 29, 277–282.
Liu, P., Luo, L., Chao-an, L., (2013). Characterization of Competition for nutrients in the biocontrol of Penicillium
italicum by Kloeckera apiculate, Biological Control, 67, 157–162.
Lutz, M.C., Lopes, C.A., Rodriquez, M.E., Sosa, M.C., and Sangorrin, M.P. (2013). Efficacy and putative mode of
action of native and commercial antagonistic yeasts against postharvest pathogens of pear. International
Journal of Food Microbiology, 164, 166-172.
Nally M.C., Peace, V.M., Maturano, Y.P., Munoz, C.J., Combina, M., Toro, M.E., Figueroa, L.I.C., and Vazquez, F.
(2012). Biocontrol of Botrytis cinerea in table grapes by non-pathogenic indigenous Saccharomyces
cerevisiae yeasts isolated from viticultural environments in Argentina. Postharvest Biology and Technology, 64(1), 40-48.
Pantelides, L. S., Christou, O., Tsolakidon, M-D., Tsaltas, D. and Ioannou, N. (2015). Isolation, identification and in
vivo screening of grapevine yeasts for the control of black aspergilli on grape. Biological Control,
88, 46-53.
Qin, X., Xiao, H., Xue, C., Yu, Z., Yang, R., Cai, Z.and Si, L. (2015) Biocontrol of gray mold in grapes with the yeast
Hanseniaspora uvarum alone and in combination with salicylic acid or sodium bicarbonate. Postharvest Biology and Technology, 100, 160-167.
Sansone, G., Rezza, I., Fernandez, G., Calvente, V., Benuzzi, D., and Sanz, M.I. (2011). Inhibitors of
polygalacturonase and laccase of Botrytis cinerea and their application to the control of this fungus. International Biodeterioration and Biodegradation, 65, 243-247.
Senthil, R., Prabakar, K., Rajendran, I. and Karthikeyan, G. (2011). Efficacy of different biological
control agents against major postharvest pathogens of grapes under room temperature storage conditions. Phytopathologia Mediterranean, 50, 55-65.
Spadaro, D. and Droby, S. 2016. Development of biocontrol products for postharvest diseases of fruit: the
importance of elucidating the mechanisms of action of yeast antagonists. Trends in Food Science &
Technology, 47(39-49)
Vero, S., Garmendia, G., Gonzale, M.B., Bentancur, O., and Wisniewski, M. (2013). Evaluation of yeasts obtained
from Antarctic soil samples as biocontrol agents for the management of postharvest diseases of apple.
FEMS Yeast Research, 13, 189-199.
Wang, Y., Yu, T., Xia, J., Yu, D., Wang, J. and Zheng, X. (2010). Biocontrol of postharvest gray mold of cherry
tomatoes with the marine yeast Rhodosporidium paludigenum. Biological Control, 53, 178-182.
Zhang, D., Spadaro, D., Garibaldi, A., and Gullino, L.M. (2011). Potential biocontrol activity of a Pichia
guilliermondii against grey mold of apple and its possible modes of action. Biological Control, 57,
193-201.
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2017-07-26
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บทความวิจัยจากการประชุมวิชาการระดับชาติ"วิทยาศาสตร์วิจัย"ครั้งที่ 9
