Effects of Some Complex Organic Nitrogenous Substances on Growth of Cymbidium seidenfadenii (P.J.Cribb & Du Puy) P.J.Cribb Protocorms and Seedlings in vitro
Abstract
In vitro protocorms and seedlings of Cymbidium seidenfadenii (P.J.Cribb & Du Puy) P.J.Cribb were cultured on semi-solid MS medium supplemented with 0.5, 1.0 and 2.0 g/L peptone, casein hydrolysate and yeast extract respectively for 12 weeks. The results showed that the highest number of protocorms multiplication could induce when cultured on the medium added with 2.0 g/L casein hydrolysate or 0.5 g/L yeast extract (7.1+1.7 and 7.1+1.0 protocorms, respectively). However, the highest number of shoots and leaves derived from protocorms (2.1 + 0.7 shoots and 4.3 + 1.4 leaves, respectively) were found on the medium without organic substances. In case of seedling explants, the highest number of protocorms (2.1 + 1.0 protocorms) was observed on the augment medium with 1.0 g/L casein hydrolysate. However, the highest leaf and shoot length (0.87 + 0.04 cm and 2.81 + 0.01 cm, respectively) was noticed on the medium supplemented with 2.0 g/L Peptone. Keywords: Dendrobium seidenfadenii (P.J.Cribb & Du Puy) P.J.Cribb, complex organic nitrogenous substances, growthReferences
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Sinha, P. & Roy, S.K. (2004). Regeneration of an indigenous orchid, Vanda teres (Roxb.) Lindl. through
in vitro culture. Plant Tissue Culture, 14(1), 55-61.
Srivastava, H.S. & Singh, R.P. (1999). Nitrogen nutrition and plant growth. Science Publishers, Inc., United States of America.
Swarts, N.D. & Dixon, K.W. (2009). Perspectives on orchid conservation in botanic gardens. Trends in Plant Science, 14(11), 590-598.
Thaithong, O. (2001). Thai orchids (2nd ed). Bangkok: Baan Lae Suan Printing.
Zhang, Y.Y., Wu, K.L., Zhang, J.X., Deng, R., Duan, J., da Silva, J.A.T., Huang, W.C. & Zeng, S. (2015).
Embryo development in association with asymbiotic seed germination in vitro of Paphiopedilum
armeniacum S.C.. Scientific Reports, 1-15.
Zhongjian, L., Kewei, L., Lijun, C., Sipeng, L., Liqiang, L., Xiaochun, S. & Laiqiang, H. (2006). Conservation ecology of endangered species Paphiopedilum armeniacum (Orchidaceae). Acta Ecologica Sinica, 26(9), 2791-2800.
Arditti, J. (2008). Micropropagation of orchids (2nd ed). Blackwell Publishing Ltd.
Chanchaichaowiwat, A. (2010). Yeast extract. Modernize Science Journal, 10(2), 84-89. (in Thai)
Chen, J.T. & Chang, W.C. (2002). Effects of tissue culture conditions and explant characteristics on direct
somatic embryogenesis in Oncidium ‘Gower Ramsey’. Plant Cell, Tissue and Organ Culture, 69,
41-44.
Cribb, P.J., Dixon, K.W., Kell, S.P. & Barrett, R.L. (2003). Orchid Conservation. Natural History Publications, Kota Kinabalu, Sabah.
Da Silva, J.A.T. (2013). Orchid: Advances in tissue culture, genetics, phytochemistry and transgenic biotechnology. Floriculture and Ornamental Biotechnology, 7(1), 1-52.
Dodds, J.H. & Roberts, L.W. (1995). Experiments in plant tissue culture. Cambridge University Press, Australia.
Dressler, R.L. (1993). Phylogeny and classification of the orchid family. Cambridge University Press, Australia.
George, E.F., Hall, M.A. & Klerk, G-J.D. (2008). Plant propagation by tissue culture (3rded), Volume 1 (Background). Published by Springer, Netherlands.
Molnar, Z., Virag, E. & Ordog, V. (2011). Natural substances in tissue culture media of higher plants. Acta Biologica Szegediensis, 55(1), 123-127.
Ng, C.Y., Saleh, N.M. & Zaman, F.Q. (2010). In vitro multiplication of the rare and endangered slipper orchid, Paphiopedilum rothschildianum (Orchidaceae). African Journal of Biotechnology, 9(14), 2062-2068.
Parthibhan, S., Kumar, T.S. & Rao, M.V. (2014). Phenology and reintroduction strategies for Dendrobium aqueum Lindley – An endemic, near threatened orchid. Journal for Nature Conservation, 24, 68-71.
Persson, J., Gardestrom, P. & Nasholm, T. (2006). Uptake, metabolism and distribution of organic and inorganic nitrogen sources by Pinus sylvestris. Journal of Experimental Botany, 57, 2651-2659.
Stewart, S.L. (2007). Integrated conservation of Florida Orchidaceae in the genera Habenaria and Spiranthes: model orchid conservation systems for the Americas. University of Florida.
Sinha, P. & Roy, S.K. (2004). Regeneration of an indigenous orchid, Vanda teres (Roxb.) Lindl. through
in vitro culture. Plant Tissue Culture, 14(1), 55-61.
Srivastava, H.S. & Singh, R.P. (1999). Nitrogen nutrition and plant growth. Science Publishers, Inc., United States of America.
Swarts, N.D. & Dixon, K.W. (2009). Perspectives on orchid conservation in botanic gardens. Trends in Plant Science, 14(11), 590-598.
Thaithong, O. (2001). Thai orchids (2nd ed). Bangkok: Baan Lae Suan Printing.
Zhang, Y.Y., Wu, K.L., Zhang, J.X., Deng, R., Duan, J., da Silva, J.A.T., Huang, W.C. & Zeng, S. (2015).
Embryo development in association with asymbiotic seed germination in vitro of Paphiopedilum
armeniacum S.C.. Scientific Reports, 1-15.
Zhongjian, L., Kewei, L., Lijun, C., Sipeng, L., Liqiang, L., Xiaochun, S. & Laiqiang, H. (2006). Conservation ecology of endangered species Paphiopedilum armeniacum (Orchidaceae). Acta Ecologica Sinica, 26(9), 2791-2800.
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2017-06-27
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บทความวิจัยจากการประชุมวิชาการระดับชาติ"วิทยาศาสตร์วิจัย"ครั้งที่ 9
