Antibacterial and Antioxidant Activities of Cerbera manghas and C. odollam Leaf Extracts
Abstract
Aim of this research is to evaluate antibacterial activity of C. manghas and C. odollam leaf extracts against plant and animal pathogens, and also antioxidant activities. Using turbidimetric assay, X. campestris pv. vesicatoria. growth showed the highest reduction by ethyl acetate extract (EA) of C. odollam (65.27%) than that of C. manghas (48.23%). EA also exhibited the highest antioxidant activity by scavenging DPPH radical with the EC50 of 0.55±0.03 mg/mL and had the strongest reducing ability (2.08±0.42 mg ascorbic acid equivalent/g extract). However, hexane extract (Hex) was found to be the best Fe2+ chelator. Moreover, bioactivity assays revealed positive correlation with total phenolic and flavonoid contents of the extracts, indicating that both compounds take into account for these activities. Our findings suggest that C. odollam leaf extracts are useful for future development for medical and agricultural purposes. Keywords : Cerbera manghas, Cerbera odollam, antibacterial, antioxidantReferences
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Xiaoyong, S. and Luming, C. (2014). Phenolic constituents, antimicrobial and antioxidant properties of blueberry leaves (V5). Journal of Food and Nutrition Research, 2(12), 973-979.
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Benzie, I.F. and Strain, J.J. (1999). Ferric reducing/antioxidant power assay: direct measure of total antioxidant activity of biological fluids and modified version for simultaneous measurement of total antioxidant power and ascorbic acid concentration. Methods in Enzymology, 299, 15-27.
Carter, P. (1971) Spectrophotometric determination of serum iron at the submicrogram level with a new reagent (ferrozine). Analalytical Biochemistry, 40, 450–458.
Chan, E.W.C., Lim, Y.Y. and Omar, M. (2007). Antioxidant and antibacterial activity of leaves of Etlingera species (Zingiberaceae) in Peninsular Malaysia. Food Chemistry, 104(4), 1586-1593.
CLSI. (2012). Performance Standards for Antimicrobial Susceptibility testing; Twenty-Second Informational Supplement. CLSI document M100-S22. Pennsylvania: Clinical and Laboratory Standards Institute.
Maddox, C.E., Laur, L.M., and Tian, L. (2010). Antibacterial activity of phenolic compounds against the phytopathogen Xylella fastidiosa. Current Microbiology, 60(1), 53-58.
Molyneux, P. (2004). The use of the stable free radical diphenylpicrylhydrazyl (DPPH) for estimating antioxidant activity. Songklanakarin Journal of Science and Technology, 26(2), 211-219.
Orhan, D.D., Özçelik, B., Özgen, S. and Ergun, F. (2010). Antibacterial, antifungal, and antiviral activities of some flavonoids. Microbiological Research, 165(6), 496-504.
Osei-Djarbeng, S.N., Cutler, S.J., Cutler, R.R. and Corcoran, O. (2014). Fibroblast growth stimulation, DPPH antioxidant assay and antimicrobial activities of Funtumia elastica (Preuss) Stapf (Apocynaceae) leaf extracts. European Journal of Medicinal Plants, 4(7), 835-843.
Pandey, K.B. and Rizvi, S.I. (2009). Plant polyphenols as dietary antioxidants in human health and disease. Oxidative Medicine and Cellular Longevity, 2(5), 270-278.
Quattrocchi, U. (2012). CRC World Dictionary of Medicinal and Poisonous Plants: Common Names, Scientific names, Eponyms, Synonyms and Etymology. Florida: CRC Press.
Rattanasena, P. (2012). Antioxidant and antibacterial activities of vegetables and fruits commonly consumed in Thailand. Pakistan Journal of Biological Sciences, 15(18), 877-882.
Saeed, N., Khan, M.R. and Shabbir, M. (2012). Antioxidant activity, total phenolic and total flavonoid contents of whole plant extracts Torilis leptophylla L. BMC Complementary and Alternative Medicine, 12(221), 1-12.
Sathishkumar, T. and Baskar, R. (2012). Evaluation of antioxidant properties of Tabernaemontana heyneana Wall. leaves. Indian Journal of Natural Products and Resources. 3(2), 197-207.
Sengul, M., Ercisli, S., Yildiz, H., Gungor, N., Kavaz, A., and Çetin, B. (2010). Antioxidant, antimicrobial activity and total phenolic content within the aerial parts of Artemisia absinthum, Artemisia santonicum and Saponaria officinalis. Iranian Journal of Pharmaceutical Research, 10(1), 49-56.
Singleton, V.L., Orthofer, R. and Lamuela-Raventos, R.M. (1999). Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Methods in Enzymology, 299,
152-178.
Somwang, T. & Wanichwatanadecha, P. (2015). Comparative study of antibacterial activity of extracts from Cerbera odollam and Cerbera manghas leaves. In Proceedings of The 7th National Science Research Conference. (pp. 1-5). Phitsanulok: Naresuan University. (in Thai)
Thakurdesai, P.A., Narayanan, L.S. and Mazumder, P.M. (2010). In vitro antioxidant and free radical scavenging activity of fractions from Alstonia scholaris Linn. R. Br. American International Journal of Pharmaceutical Technology Research, 2(1), 18-25.
Wanichwatanadecha, P., Kasempin, N., Wilatong, S. and Khoonthong, W. (2015) Antibacterial activity of Cerbera odollam Gaertn. extract against plant pathogenic Xanthomonas. In Proceedings of Pure and Applied Chemistry International Conference 2015 (PACCON 2015). (pp. 501-204). Bangkok: The Chemical Society of Thailand under the Patronage of Her Royal Highness Princess Chulabhorn Mahidol and Department of Chemistry, Faculty of Science, King Mongkut’s University of
Technology Thonburi.
World Health Organization. (2014). Antimicrobial resistance: global report on surveillance. Geneva:
WHO Press.
Xiaoyong, S. and Luming, C. (2014). Phenolic constituents, antimicrobial and antioxidant properties of blueberry leaves (V5). Journal of Food and Nutrition Research, 2(12), 973-979.
Xu, Y., Luo, Q.Q. and Zhou, M.G. (2013). Identification and characterization of integron-mediated antibiotic resistance in the phytopathogen Xanthomonas oryzae pv. oryzae. PloS one, 8(2), e55962.
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2017-07-17
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