Effect of Nonylphenol to Vitellogenin Induction in Juvenile Asian Sea Bass (Lates calcarifer)
Abstract
The study on effect of xenoestrogen as nonylphenol, which is a nonionic surfactant used in textiles industrial and detergent product were done. Vitellogenin was induced in juvenile Asian sea bass (Lates calcarifer) by injection with 4-Nonylphenol (25 milligram/ kilogram body weight) and 17β- estradiol (2 milligram/ kilogram body weight) as a positive control. Three days after injection, plasma were collected to detection of VTG every 3 days using Dot blot and Western blot. Result from dot blot showed the induction of vitellogenin in plasma of the nonylphenol treatment group and 17β - estradiol control group since 3 days after exposure. In addition, Western blot can detect vitellogenin induction only in 17β- estradiol injection groups. These results showed that vitellogenin can be induced with nonylphenol likewise 17β- estradiol (2 milligram/ kilogram body weight) and, detected by dot blot. For these results, this vitellogenin detection could be applied for detection of estrogenic effect in aquatic environment as well. Keywords : vitellogenin, asian sea bass, biomarker, 17β- estradiol, nonylphenolReferences
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(pp. 491-496) Payao : University of Payao. (In Thai)
Ritphoe, S., Pimprong, A., Prasatkaew, W. and Nanthanawat P. (2015). Purification and Characterization of Plasma Vitellogenin from Asian Sea Bass (Lates calcarifer) In Proceedings the 7th National Science Research Conference (BI-P002 p1-7) Pitsanulok : Naresuan University. (In Thai)
Vaccaro, E., Meucci, V., Intorre, L., Soldani, G., Bello, D.D., Longoa, V., Gervasi, P.G. and Pretti, C. (2005). Effects of 17-estradiol, 4-nonylphenol and PCB 126 on the estrogenic activity and phase 1 and 2 biotransformation enzymes in male sea bass (Dicentrarchus labrax). Aquatic Toxicology,
75, 293-305.
.
Maltais, D. and Roy R.L. (2009). Purification and partial characterization of vitellogenin from shorthead redhorse (Moxostoma macrolepidotum) and copper redhorse (Moxostoma hubbsi) and detection in plasma and mucus with heterologous antibody. Fish Physiology and Biochemistry, 35(2), 241-254.
Nanthanawat, P. (2015). Vitellogenin : Biomarker for EDCs Exposure in Aquatic Environment Burapha Science Journal, 20(2), 201-208. (In Thai)
Pan, Z., Tain, H., Wang, W., Wang, J. and Ru, S. (2012). Identification, Purification, and Immunoassay of Stone Flounder (Kareius bicolouratus) Vitellogenin. Journal of Korean Society for Applied Biological Chemistry, 55(2), 219-227.
Prasatkaew, W., Thanomsit, C. and Nanthanawat, P. (2016). Cross-Reactivity of Monoclonal Antibody against Vitellogenin from Asian Sea bass. In Proceedings the 8th Science Research Conference
(pp. 491-496) Payao : University of Payao. (In Thai)
Ritphoe, S., Pimprong, A., Prasatkaew, W. and Nanthanawat P. (2015). Purification and Characterization of Plasma Vitellogenin from Asian Sea Bass (Lates calcarifer) In Proceedings the 7th National Science Research Conference (BI-P002 p1-7) Pitsanulok : Naresuan University. (In Thai)
Vaccaro, E., Meucci, V., Intorre, L., Soldani, G., Bello, D.D., Longoa, V., Gervasi, P.G. and Pretti, C. (2005). Effects of 17-estradiol, 4-nonylphenol and PCB 126 on the estrogenic activity and phase 1 and 2 biotransformation enzymes in male sea bass (Dicentrarchus labrax). Aquatic Toxicology,
75, 293-305.
.
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Published
2017-07-20
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บทความวิจัยจากการประชุมวิชาการระดับชาติ"วิทยาศาสตร์วิจัย"ครั้งที่ 9
