Ontogeny of Digestive Enzymes in Spotted Scat (Scatophagus argus Linnaeus, 1766)
Abstract
The study aimed at examining digestive enzyme activities of pepsin, trypsin, lipase and amylase in spotted scat from hatching to 30 days post hatching (DPH). Optimal pH for each digestive enzyme activity was determined in the first step. Subsequently, analysis of activities of the four enzymes were followed in the larval fish digestive system. For the optimal pH study, the highest pepsin activities (178.20±19.59 unit/mg protein) in fish stomach were at pH 2.0 (P<0.05). In pyloric caeca and intestine, the highest trypsin activities were at pH 8.0 (7.71±1.46 and 13.21±0.51 unit/mg protein, respectively). The highest lipase activities (P<0.05) in pyloric caeca were at pH 8.5 (3.13±0.78 unit/mg protein) while those in intestine at pH 8.0 (0.70±0.15 unit/mg protein). The highest amylase activities were at pH 7.0 (177.79±15.38 and 115.77±7.20 unit/mg protein, (P<0.05)) in pyloric caeca and intestine, respectively. Investigation on digestive enzyme activities from hatching (0 DPH) to 30 DPH (average body length and weight of 8.50±0.50 mm, 28.67±1.41 mg, respectively) showed that pepsin activities increased at a constant rate until 30 DPH. Trypsin activities slowly increased from hatching until 15 DPH and risened rapidly from 17-30 DPH while those of lipase exhibited an increasing trend from hatching and a sharp increase at 30 DPH. Amylase activities appeared at low levels during 3-4 DPH then, increased slowly during 5-21 DPH and rapidly amplified during 24-30 DPH. The results from the present study are useful for estimating larval fish digestibility and growth that are fundamental for development of suitable feed for larval stages of spotted scat.References
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