Effects of Media and Plant Growth Regulators on in vitro Seedling Culture of Dendrobium moschatum (Buch.-Ham.) Sw.
Abstract
This study aimed to investigate the effect of media and plant growth regulators on the growth of in vitro seedling culture of Dendrobium moschatum (Buch.-Ham.) Sw. The 1 cm height seedlings obtained from seeds in vitro conditions were grown on different media (MS, ½ MS, VW & ½ VW). After 12 weeks of cultivation, the results clearly showed that seedlings on ½VW medium showed the highest plant height (2.19 cm). The same medium was also able to induce new shoots and new roots at 100% with an average number of new shoots and new roots of 1.72 shoots/plant and 5.72 roots/plant, respectively. Further study was done using the same size seedlings cultured on ½VW medium supplemented with auxins (IAA, IBA, NAA) or cytokinins (BA, kinetin, TDZ) at the concentration of 0, 0.1, 0.5, 1.0, and 2.0 mg/L for 12 weeks. The results demonstrated that the new shoot induction and root induction rate of 93.75% and 100% with an average number of new shoots and new roots of 4.70 shoots/plant and 13.63 roots/plant were observed when cultured on the ½VW medium supplemented with 1.0 mg/L IBA. In the case of cytokinins, the results showed that the new shoot induction rate and new root induction rate were at 100% with an average number of new shoots and new roots of 3.99 shoots/plant and 6.55 roots/plant were obtained when cultured on the ½VW medium adding with 1.0 mg/L kinetin. Our results can be used as a method for cultivation in large quantities to use this plant for other purposes, especially for species conservation.References
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