Selection, Classification, and Study of the Properties of Streptomyces sp. SAURU59 against Pathogenic Fungi Causing Durian Root and Stem Rot Disease
Abstract
This research aimed to select an actinomycete strain from soil collected in Nan River National Park, Tha Pla District, Uttaradit Province, using the soil dilution plate method and dual culture technique to examine Phytophthora palmivora, the causative agent of durian root and stem rot disease. The research also included the identification of antagonistic actinomycetes and an investigation into the biochemical properties of these actinomycetes. A total of 69 isolates were obtained from the soil, with only isolate SAURU59 found to exhibit the highest efficiency in inhibiting the growth of the fungal pathogen, achieving a 75 percent inhibition rate. Morphological characterization and analysis of the 16S rRNA nucleotide sequence revealed that SAURU59 was gram-positive and had spores formed in a chain-like structure with a spiral and smooth surface. Additionally, the nucleotide sequence of SAURU59 showed similarity to Streptomyces rimosus. This isolate showed the ability to produce cellulase and chitinase enzymes, as well as 30.78 µg/ml of indole-3-acetic acid (IAA) within 10 days.References
Chantarasiri, A., & Boontanom, P. (2021) Fusarium solani and Lasiodiplodia pseudotheobromae, fungal pathogens causing stem rot disease on durian trees (Durio zibethinus) in Eastern Thailand. New Disease Report, 44, 1 – 3.
Department of Agriculture. (2019) Durian disease. Department of Agriculture Report. 78 p. (in Thai)
Doyle, J. J., & Doyle, J. L. (1990) Isolation of plant DNA from fresh tissue. Focus, 12, 13 - 15.
Hassan, N., Nakasuji, S., Elsharkawy, M. M., Naznin, H. A., Kubota, M., Ketta, H., & Shimizu, M. (2017) Biocontrol potential of an endophytic Streptomyces sp. strain MBCN152-1 against Alternaria brassicicola on cabbage plug seedlings. Microbes and Environments, 32 (2), 133 – 141.
Himaman, W., Thamchaipenet, A., Pathom-aree, W., & Duangmal, K. (2016) Actinomycetes from Eucalyptus and their biological activities for controlling Eucalyptus leaf and shoot blight. Microbiological Research,188, 42 - 52.
Ibrahim, A. A., El-Housseiny, G. S., Aboshanab, K. M., Yassien, M. A. & Hassouna, N. A. (2019) Paromomycin production from Streptomyces rimosus NRRL 2455: statistical optimization and new synergistic antibiotic combinations against multidrug resistant pathogens. BMC Microbiology, 19 (18), 1 – 15.
Kaewnamarng, S., Akarapisan, A., & Ruangwong, O. (2018) Selection of rhizobacteria to control fruit blotch of melon. Journal of Agriculture, 34 (2), 193 – 204. (in Thai)
Kelly, K. L. (1958) Central notations for the revised ISCC-NBS color name blocks. Journal of Research of the National Bureau of Standard, 61 (5), 427 – 431.
Khompun, W., & Thabthim, S. (2016) Effect of actinomycetes isolated from rhizospheric soil of chilli on growth inhibition of Colletotrichum capsici, Curvularia lunata and Fusarium solani. Khon Khan Agricultural Journal, 1, 942 – 947. (in Thai)
Lu, D., Ma, Z., Xu, X., & Yu, X. (2016) Isolation and identification of biocontrol agent Streptomyces rimosus M527 against Fusarium oxysporum f. sp. cucumerinum. Journal Basic Microbiology. 56, 929 – 933.
Petkovic, H., Cullum, J., Hranueli, D., Hunter, I. S., Peric´-Concha, N., Pigac, J., Thamchaipenet, A., Vujaklija, D., & Long, P. F. (2006) Genetics of Streptomyces rimosus, the Oxytetracycline producer. Microbiology and Molecular Biology Reviews, 70(3), 704 – 728.
Piasai, O., & Sudsanguan, M. (2018) Morphological study of Gelasinospora from dung and antagonistic effect against plant pathogenic fungi in vitro. Agriculture and Natural Resources, 52, 407 – 411.
Sari, M., Nawangsih A. A., & Wahyudi A. T. (2021) Rhizosphere Streptomyces formulas as the biological control agent of phytopathogenic fungi Fusarium oxysporum and plant growth promoter of soybean. Biodiversitas, 22 (6), 3015 – 3023.
Soytong, K. (2010) Evaluation of Chaetomium - biological fungicide to control Phytophthora stem and root rot of durian. Rajamangala University of Technology Tawan-ok Research journal, 3 (2), 115 – 124.
Suksiri, S., Laipasu, P., Soytong, K., & Poeaim, S. (2018) Isolation and identification of Phytophthora sp. and Pythium sp. from durian orchard in Chumphon Province, Thailand. International Journal of Agricultural Technology, 14 (3), 389 – 402.
Sunpapao, A., Chairina, T., & Ito, S. (2018) The biocontrol by Streptomyces and Trichoderma of leaf spot disease caused by Curvularia oryzae in oil palm seedlings. Biological Control, 123, 36 – 42.
Torres-Rodriguez, J. A., Reyes-Pérez, J.J., Quiñones-Aguilar, E.E., & Hernandez-Montiel, L.G. (2020) Actinomycete potential as biocontrol agent of phytopathogenic fungi: mechanisms, source, and applications. Plants, 11, 1 - 15.
Department of Agriculture. (2019) Durian disease. Department of Agriculture Report. 78 p. (in Thai)
Doyle, J. J., & Doyle, J. L. (1990) Isolation of plant DNA from fresh tissue. Focus, 12, 13 - 15.
Hassan, N., Nakasuji, S., Elsharkawy, M. M., Naznin, H. A., Kubota, M., Ketta, H., & Shimizu, M. (2017) Biocontrol potential of an endophytic Streptomyces sp. strain MBCN152-1 against Alternaria brassicicola on cabbage plug seedlings. Microbes and Environments, 32 (2), 133 – 141.
Himaman, W., Thamchaipenet, A., Pathom-aree, W., & Duangmal, K. (2016) Actinomycetes from Eucalyptus and their biological activities for controlling Eucalyptus leaf and shoot blight. Microbiological Research,188, 42 - 52.
Ibrahim, A. A., El-Housseiny, G. S., Aboshanab, K. M., Yassien, M. A. & Hassouna, N. A. (2019) Paromomycin production from Streptomyces rimosus NRRL 2455: statistical optimization and new synergistic antibiotic combinations against multidrug resistant pathogens. BMC Microbiology, 19 (18), 1 – 15.
Kaewnamarng, S., Akarapisan, A., & Ruangwong, O. (2018) Selection of rhizobacteria to control fruit blotch of melon. Journal of Agriculture, 34 (2), 193 – 204. (in Thai)
Kelly, K. L. (1958) Central notations for the revised ISCC-NBS color name blocks. Journal of Research of the National Bureau of Standard, 61 (5), 427 – 431.
Khompun, W., & Thabthim, S. (2016) Effect of actinomycetes isolated from rhizospheric soil of chilli on growth inhibition of Colletotrichum capsici, Curvularia lunata and Fusarium solani. Khon Khan Agricultural Journal, 1, 942 – 947. (in Thai)
Lu, D., Ma, Z., Xu, X., & Yu, X. (2016) Isolation and identification of biocontrol agent Streptomyces rimosus M527 against Fusarium oxysporum f. sp. cucumerinum. Journal Basic Microbiology. 56, 929 – 933.
Petkovic, H., Cullum, J., Hranueli, D., Hunter, I. S., Peric´-Concha, N., Pigac, J., Thamchaipenet, A., Vujaklija, D., & Long, P. F. (2006) Genetics of Streptomyces rimosus, the Oxytetracycline producer. Microbiology and Molecular Biology Reviews, 70(3), 704 – 728.
Piasai, O., & Sudsanguan, M. (2018) Morphological study of Gelasinospora from dung and antagonistic effect against plant pathogenic fungi in vitro. Agriculture and Natural Resources, 52, 407 – 411.
Sari, M., Nawangsih A. A., & Wahyudi A. T. (2021) Rhizosphere Streptomyces formulas as the biological control agent of phytopathogenic fungi Fusarium oxysporum and plant growth promoter of soybean. Biodiversitas, 22 (6), 3015 – 3023.
Soytong, K. (2010) Evaluation of Chaetomium - biological fungicide to control Phytophthora stem and root rot of durian. Rajamangala University of Technology Tawan-ok Research journal, 3 (2), 115 – 124.
Suksiri, S., Laipasu, P., Soytong, K., & Poeaim, S. (2018) Isolation and identification of Phytophthora sp. and Pythium sp. from durian orchard in Chumphon Province, Thailand. International Journal of Agricultural Technology, 14 (3), 389 – 402.
Sunpapao, A., Chairina, T., & Ito, S. (2018) The biocontrol by Streptomyces and Trichoderma of leaf spot disease caused by Curvularia oryzae in oil palm seedlings. Biological Control, 123, 36 – 42.
Torres-Rodriguez, J. A., Reyes-Pérez, J.J., Quiñones-Aguilar, E.E., & Hernandez-Montiel, L.G. (2020) Actinomycete potential as biocontrol agent of phytopathogenic fungi: mechanisms, source, and applications. Plants, 11, 1 - 15.
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Published
2023-10-09
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