Allelopathic Effect from Synedrella nodiflora (L.) Gaertn.) on Seed-Germinating Physiology of Pakchoi (Brassica chinensis Jusl var parachinensis (Bailey) Tsen & Lee)
Abstract
Allelopathy is a natural phenomenon that plants release allelopathics to inhibit neighbor plants to reduce the resources revening. This can be developed and use in agriculture. In this study, the Synedrella nodiflora (L.) Gaertn. ethanol extract was used to test seed germination and growth of pakchoi (Brassica chinensis Jusl var parachinensis (Bailey) Tsen & Lee) at the concentration range between 1.3 and 13.0 mg/mL for 7 days. The results show that the IC50 of extract was 2.96 mg/mL. and the osmotic potential was -0.08 MPa. The physiology of seed gemination was tested. Seeds were geminated in IC50 of extract , sucrose, NaCl, and KNO3 (with -0.08 MPa osmotic potential). The results revealed that the percent of gemination and growth of seed soaked in water, sucrose, NaCl, and KNO3 were not different but statistic significant different (P≤0.05) from extract treated seeds. Seed water absorption in extract, sucrose, NaCl, and KNO3 was determined and compared with water for 0-24 hour. This show that seed water absorption was not statistically different at all time points. The change of seed storages was also determined in seeds treated by the extract (at IC50) compared to the control for 7 days. Extract treated seed showed higher starch content than the control but lower reducing sugar content. The protein content of treated seeds was not statistically different from untreated dry seeds, but the control had statistic significant lower protein content (P≤0.05). In conclude, the extract can inhibit pakchoi gemination which no effect on water absorption but showed the effect on food storages utilization during germination.References
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