Effects of Protease and Phytase Supplemented in Pellet Feed on Growth Performance, Survival Rate, Feed Conversion Ratio, Feed Efficiency and Feed Digestibility of Hybrid Catfish (Clarias macrocephalus X Clarias gariepinus)


  • Boonyarath Pratoomchat Faculty of Science, Burapha University


The effect of inclusion of protease and phytase on growth performance, survival rate, feed utilization and apparent digestibility of dry matter (ADMD), protein (ACPD) and lipid (ALD) of hybrid catfish (Clarias macrocephalus X Clarias gariepinus), the four experimental feeds were designed by CRD (Completely Randomized Design) as protease levels supplemented in feed formular as followed 500 U/kg (T1), 1,000 U/Kg (T2) 2,000 U/kg (T3) and plus 500 U/kg phytase of these formulas and without enzyme supplementation (C). Enzymes was thoroughly mixed in finely ground feed with 31.98±0.06% protein followed by chromic oxide at 0.5% dry weight and thoroughly mixed, pelleting, drying, then coating with fish liver oil at 0.5% dry weight. The hybrid catfish at initial size of 5.45±0.05 g and 9.22±0.04 cm in size was raised in 1m3 concrete tank at density of 30 fish/m2. They were fed at 5% of body wet weight and three times daily. The three replications were applied. Ten fishes per replication (33%) were randomly sampled for size evaluation every ten days until at day 70 of culture, the fish fecal was collected for digestibility test. Results found that survival rate, %weight and length gains, specific growth rate, size variation and feed conversion ratio were not different (p>0.05) among groups. Average daily weight gain, feed efficiency, feed consumption, ADMD and ACPD of fish fed with feed mixed with 500 U/kg protease and 500 U/kg phytase were significantly higher (p<0.05) than that of control feed but not different (p>0.05) with feed mixed with 1,000 U/kg protease and 500 U/kg phytase and feed mixed with 2,000 U/kg protease and 500 U/kg phytase. ALD of fish fed with feed mixed with 500 U/kg,  1,000 U/kg , and  2,000 U/kg protease and 500 U/kg phytase was significantly higher (p<0.05) than that of control feed. This indicates that the supplementation of protease 500 U/kg and phytase 500 U/kg in feed is the most suitable level for raising this species in terms of growth performance and feed utilization.


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