Effect of Cyanobacteria Limnothrix Strain LmTK01 on the Survival Rate of Shrimp Litopenaeus vannamei and Factors Effected on LmTK01 Growth

Authors

  • Kanokwan Maa-iad Faculty of Natural Resources, Prince of Songkla University
  • Teeyaporn Keawtawee
  • Teeyaporn Keawtawee
  • Mintra Seel-audom
  • Mintra Seel-audom

Abstract

Cyanobacteria Limnothrix strain LmTK01 is a recently discovered cyanobacteria in Pacific white shrimp ponds (Litopenaeus vannamei), which causes shrimp production decrease. The morphology of LmTK01 is similar to cyanobacteria genus Limnothrix. The aims of this study were to investigate the effect of LmTK01 on the survival rate of shrimp (L. vannamei) and the factors of temperature, pH and salinity effected by LmTK01 growth. Algae LmTK01 were isolated from shrimp water culture during its blooming in the shrimp ponds. Algae were stocked in sterilization liquid BG-11 medium under light intensity 2,000 - 3,000 lux in a light: dark cycle, 12h: 12h. Different density of LmTK01 (336.17, 703.70 and 1,390.63 µg Chl-a/L) were inoculated with 0.18 ± 0.05 g of shrimp in the individual 500 mL of seawater for 96 hours. Water quality and survival rate of shrimp were measured every 6 hours. Results showed that shrimp died in over 50% of all treatments were inoculated with LmTK01. Algae LmTK01 showed the stress effect on shrimp directly and indirectly. The direct cause was algae clogging at the shrimp’s gill and gastrointestinal tract, which interferes with the shrimp's breathing and digestion, while the indirect cause was changing in water quality, such as an increase in the pH value. These results caused the shrimp become to stressed, weak and eventually die.  Moreover, it was found that LmTK01 could grow well in a temperature of 28.00 °C, pH 7- 8 and salinity 0 - 30 ppt. Therefore, these results indicated that LmTK01 showed a significantly bad effect on the survival rate of shrimp, resulting in loss of shrimp production.Keywords :  Cyanobacteria; Pacific white shrimp; Water quality

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Published

2023-05-11