Effect of Different Coating Materials on the Survival of Lactobacillus plantarum and Quality of Mango Sorbet

Authors

  • Thippharak Wongsadee คณะวิทยาศาสตร์และเทคโนโลยี มหาวิทยาลัยราชภัฏบ้านสมเด็จเจ้าพระยา
  • Makkala Jang-im
  • Auemporn Santhana
  • Nawaporn Hongpan

Abstract

The objectives of this research were to study the effect of different encapsulation materials (free cell, alginate, alginate-fructooligosaccharide, alginate-chitosan, and alginate-fructooligosaccharide-chitosan) on encapsulation yield, size of probiotic beads, the survival of encapsulated probiotic Lactobacillus plantarum in simulated gastrointestinal conditions, and protection of probiotic beads from gallic acid. The qualities of mango sorbet fortified with L. plantarum (encapsulated or free cells) and viability of L. plantarum in mango sorbet during  5 weeks storage at -18±2°C were evaluated. The research results showed that the addition of fructo-oligosaccharide during microencapsulation of L. plantarum increased the size of the alginate beads from 2.91 to 3.11 millimeter (p<0.05). The beads of alginate with fructooligosaccharide and chitosan coating showed the highest survival of L. plantarum in simulated gastrointestinal conditions (p<0.05). In addition, the chitosan coating of alginate beads gave better protection of the penetration of a gallic acid within the beads than all types of beads. The addition of all types of beads significantly increased the melting rate of mango sorbet whereas the texture, and overall scores of mango sorbet decreased compared to free cells (p<0.05). Furthermore, the chitosan coating of alginate beads provided better L. plantarum survival in mango sorbet during storage at                     -18±2 °C. Conclusively, the beads of alginate with fructooligosaccharide and chitosan coating showed the lowest losses of the counts of total L. plantarum from the encapsulation to simulated gastrointestinal conditions.          Keywords :  encapsulation ; probiotic ; fructooligosaccharide ; chitosan ; sorbet

Author Biography

Thippharak Wongsadee, คณะวิทยาศาสตร์และเทคโนโลยี มหาวิทยาลัยราชภัฏบ้านสมเด็จเจ้าพระยา

    

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Published

2022-09-01