A Study of Oyster Mushrooms for Producing Cellulase Enzyme
Abstract
The objective of this research was to study three species of Pleurotus spp., are widely consumed edible mushrooms, consisting of P. sajor-caju, P. ostreatus and P. cystidiosus. Basidiocarp and spawn on sorghum seeds were collected from seven provinces in Central and Eastern of Thailand. Thirteen isolates were recovered from 15 samples which include P. sajor-caju, P. ostreatus and P. cystidiosus for 5, 6 and 2 isolates, respectively. The studies of their growth rates and enzymatic capability were performed on potato dextrose ager (PDA) and Czapek Dox Agar mixed with 1% carboxymethyl cellulose (CZ+CMC), respectively. Both media were incubated at 25ºC for seven days. The three most potential isolates were selected including P. sajor-caju PS5, P. ostreatus PO7 and P. cystidiosus PC1. Therefore, these mushrooms have potential for development in the future. Because they can grow rapidly and produce cellulase effectively. Keywords : oyster mushroom ; cellulase ; edible mushroom ; PleurotusReferences
Aschariyaphotha W., Wattanuruk, D. & Nilrit, P. (2018). Cellulase activity assay from woody destroying fungi in alcohol production from water hyacinth (Eichornia crassipes) by simultaneous saccharification and fermentation. VRU Research and Development Journal, 13(3), 23-33. (in Thai)
Atri, N.S. & Sharma, S.K. (2012). Qualitative estimation of cellulases and lignin modifying enzymes in five wild fungal species selected from Northern West India. Academic Journal of Plant Sciences, 5 (1), 23-27.
Baldrian, P. & Gabriel, J. (2003). Lignocellulose degradation by Pleurotus ostreatus in the presence of cadmium. FEMS Microbiology Letters, 220, 235-240.
Hoa, H.T. & Wang C.L. (2015). The effects of temperature and nutritional conditions on mycelium growth of two oyster mushrooms (Pleurotus osteratus and Pleurotus cystidiosus). Mycobiology, 43(1), 14-23.
Mahadevan, K. & Shanmugasundaram, K. (2018). Comparative effect of different culture media on mycelial growth performance of Pleurotus sapidus. Journal of Pharmacognosy and Phytochemistry, 7(4), 874-878.
Rajarathnam, S., Bano, Z. & Miles, P. (2009). Pleurotus mushrooms. Part I A. Morphology, life cycle, taxonomy, breeding and cultivation. CRC Critical Reviews in Food Science and Nutrition, 26(2), 157-223.
Saengwanich, U., Suwannarith, P., Payappanont, A., Laungsa-ard, J., Chansrikul, A. & Sakolruk, B. (2013). The List of Biological Resources – Mushrooms. Biodiversity-based Economy Development Office. (in Thai).
Thaneerananon, I. & Vilalai, P. (2019). Cost and return analysis of investment on oyster mushroom farming in Nakhon Pathom : Case study learning resources. Journal of Management Science Nakhon Pathom Rajabhat University 6 (1), 91-108. (in Thai)
Wu, Y. and Shin, H.J. (2016). Cellulase from the fruiting bodies and mycelia of edible mushrooms: A review. Journal of Mushrooms, 14(4), 127-135.
Atri, N.S. & Sharma, S.K. (2012). Qualitative estimation of cellulases and lignin modifying enzymes in five wild fungal species selected from Northern West India. Academic Journal of Plant Sciences, 5 (1), 23-27.
Baldrian, P. & Gabriel, J. (2003). Lignocellulose degradation by Pleurotus ostreatus in the presence of cadmium. FEMS Microbiology Letters, 220, 235-240.
Hoa, H.T. & Wang C.L. (2015). The effects of temperature and nutritional conditions on mycelium growth of two oyster mushrooms (Pleurotus osteratus and Pleurotus cystidiosus). Mycobiology, 43(1), 14-23.
Mahadevan, K. & Shanmugasundaram, K. (2018). Comparative effect of different culture media on mycelial growth performance of Pleurotus sapidus. Journal of Pharmacognosy and Phytochemistry, 7(4), 874-878.
Rajarathnam, S., Bano, Z. & Miles, P. (2009). Pleurotus mushrooms. Part I A. Morphology, life cycle, taxonomy, breeding and cultivation. CRC Critical Reviews in Food Science and Nutrition, 26(2), 157-223.
Saengwanich, U., Suwannarith, P., Payappanont, A., Laungsa-ard, J., Chansrikul, A. & Sakolruk, B. (2013). The List of Biological Resources – Mushrooms. Biodiversity-based Economy Development Office. (in Thai).
Thaneerananon, I. & Vilalai, P. (2019). Cost and return analysis of investment on oyster mushroom farming in Nakhon Pathom : Case study learning resources. Journal of Management Science Nakhon Pathom Rajabhat University 6 (1), 91-108. (in Thai)
Wu, Y. and Shin, H.J. (2016). Cellulase from the fruiting bodies and mycelia of edible mushrooms: A review. Journal of Mushrooms, 14(4), 127-135.
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Published
2022-09-01
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