Colorimetric Sensing of Glutathione by Copper (II) Complex with Pyrogallol Red Indicator
Abstract
In this research, the dinuclear copper (II) complex containing m-xylene units (Cu2L2) was applied to study the sensing abilities towards anions using Pyrogallol Red (PGR) as sensory unit in 10 mM HEPES buffer pH 6. The results demonstrated that the [Cu2L2•PGR] ensemble can be able to sense glutathione (GSH) selectively over than other anions, the color of [Cu2L2•PGR] change from blue to orange color of PGR in the present of GSH. The detection limit of GSH was 0.85 µM. The proposed ensemble was successfully applied to determination of glutathione in drug capsule. Keywords : Pyrogallol Red; dinuclear copper (II) complex; glutathione; ensembleReferences
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Stobiecka, M., Coopersmith, K. and Hepel, M. (2010). Resonance Elastic Light Scattering (RELS) Spectroscopy of Fast non-Langmuirian Ligand-Exchange in Glutathione-Induced Gold Nanoparticle Assembly. Journal of Colloid and Interface Science, 350, 168-177.
Teerasarunyanon, T., Watchasit, S., Suksai, C., Tuntulani, T. and Ruangpornvisuti, V. (2019). UV–vis and Theoretical Studies on an Ensemble of Dinuclear Cu(II) Complex of Anthracene–based Tripodal Tetramine with Pyrogallol Red for Cyanide Detection and Species Distribution in Aqueous Solution. Inorganic Chemistry Communications, 108, 107502.
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Watchasit, S., Suktanarak, P., Suksai, C., Ruangpornvisuti, V. and Tuntulani, T. (2014). Discriminate Sensing of Pyrophosphate Using a New Tripodal Tetramine-based Dinuclear Zn(II) Complex Under an Indicator Displacement Assay Approach. Dalton Transactions, 43, 14701-14709.
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Filomeni, G., Rotilio, G. and Ciriolo, M. R. (2005). Disulfide Relays and Phosphorylative Cascades: Partners in Redox-mediated Signaling Pathways. Cell Death and Differentiation 12, 1555–1563
Gao, W., Liu, Z., Qi, L., Lai, J., Kitte, S. A. and Xu, G. (2016). Ultrasensitive Glutathione Detection Based on Lucigenin Cathodic Electrochemiluminescence in the Presence of MnO2 Nanosheets. Analytical Chemistry, 88, 7654-7659.
Gaucher, C., Boudier, A., Bonetti, J., Clarot, I., Leroy, P. and Parent, M. (2018). Glutathione: Antioxidant Properties Dedicated to Nanotechnologies. Antioxidants, 7, 62.
Jia, R., Jin, K., Zhang, J., Zheng, X., Wang, S. and Zhang, J. (2020). Colorimetric and Fluorescent Detection of Glutathione Over Cysteine and Homocysteine with Red-emitting N-doped Carbon Dots. Sensors and Actuators B: Chemical, 321, 128506.
Freedman, J. H., Ciriolo, M. R. and Peisach, J. (1989). The Role of Glutathione in Copper Metabolism and Toxicity. The Journal of Biological Chemistry, 264, 5598-5605.
Kandar, R., Zakova, P., Lotkova, H., Kucera, O., & Cervinkova, Z. (2007). Determination of Reduced and Oxidized Glutathione in Biological Samples Using Liquid Chromatography with Fluorimetric Detection. Journal of Pharmaceutical and Biomedical Analysis, 43(4), 1382–1387.
Kennedy, L., Sandhu, J. K., Harper, M.-E., and Cuperlovic-Culf, M. (2020). Role of Glutathione in Cancer: From Mechanisms to Therapies. Biomolecules, 10, 1429.
Liu, X., Wang, Q., Zhang, Y., Zhang, L., Sub, Y. and Lv, Y. (2013). Colorimetric Detection of Glutathione in Human Blood Serum Based on The Reduction of Oxidized TMB. New Journal of Chemistry, 37, 2174-2178.
Milne, L., Nicotera, P., Orrenius, S. and Burkitt, M. J. (1993). Effect of Glutathione and Chelating Agents on Copper-Mediated DNA Oxidaion: Pro-oxidant and Antioxidant Properties of Glutathione. Archives of Biochemistry and Biophysics, 304, 102-109.
Mischley, L. K., Standish, L. J., Weiss, N. S., Padowski, J. M., Kavanagh, T. J., White, C. C., and Rosenfeld, M. E. (2016). Glutathione as a Biomarker in Parkinson’s Disease: Associations with Aging and Disease Severity. Oxidative Medicine and Cellular Longevity, 2016.
Pokharel, U. R., Fronczek, F. R., & Maverick, A. W. (2014). Reduction of Carbon Dioxide to Oxalate by a Binuclear Copper Complex. Nature Communications, 5.
Stobiecka, M., Coopersmith, K. and Hepel, M. (2010). Resonance Elastic Light Scattering (RELS) Spectroscopy of Fast non-Langmuirian Ligand-Exchange in Glutathione-Induced Gold Nanoparticle Assembly. Journal of Colloid and Interface Science, 350, 168-177.
Teerasarunyanon, T., Watchasit, S., Suksai, C., Tuntulani, T. and Ruangpornvisuti, V. (2019). UV–vis and Theoretical Studies on an Ensemble of Dinuclear Cu(II) Complex of Anthracene–based Tripodal Tetramine with Pyrogallol Red for Cyanide Detection and Species Distribution in Aqueous Solution. Inorganic Chemistry Communications, 108, 107502.
Villarama, C. D. and Maibach, H. I. (2005). Glutathione as a Depigmenting Agent: An Overview. International Journal of Cosmetic Science, 27, 147–153.
Watchasit, S., Suktanarak, P., Suksai, C., Ruangpornvisuti, V. and Tuntulani, T. (2014). Discriminate Sensing of Pyrophosphate Using a New Tripodal Tetramine-based Dinuclear Zn(II) Complex Under an Indicator Displacement Assay Approach. Dalton Transactions, 43, 14701-14709.
Yu, Y., Shi, J., Zhao, X., Yuan, Z., Lu, C. and Lu, J. (2016). Electrochemiluminescence Detection of Reduced and Oxidized Glutathione Ratio by Quantum Dot-layered Double Hydroxide Film. Analyst, 141. 3305-3312.
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2022-05-18
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