Effect of Abamectin on Alteration of Behavior, Physiology and Acetylcholinesterase Expression in Hybrid Catfish
Abstract
The aim of this study was to investigate the effects of abamectin insecticides in hybrid catfish by assessing cumulative mortality, alteration of behavior and physiology and acetylcholinesterase expression after exposing in sublethal concentration. The results indicated that cumulative mortality increased with an increasing in concentration and exposure time. The LC50 (95% confidence) at 24, 48, 72 and 96 h were 45.76 (44.61-47.01), 39.16 (38.00-40.40, 29.44 (28.42-30.46), and 20.84 (19.61-21.98) ppm. The EC50 (95% confidence) measured at 24, 48, 72 and 96 h were 4.79 (4.63-4.96), 3.72 (3.60-3.86), 2.77 (2.65-2.88), and 1.94 (1.80-2.06) ppm, respectively. Thus, we selected the hybrid catfish being exposed to abamectin in concentration of 1 ppm (sublethal concentration) for further study. The detectable change in behavior was initially found after exposing to abamectin for 6 h. We found abnormal swimming, losing balance and swimming perpendicular to the water surface. The physiological change was found after 3 h of exposure. It was found more mucus excretion. The molecular weight of acetylcholinesterase which extracted from tissues of brain, gill and muscle was studied by using Western blot technique and found that it was 71 kDa. The expression of acetylcholinesterase in these tissues tended to decrease with an increasing in exposure time. Thus, the hybrid catfish can be applied as an indicator for abamectin contamination in water. Keywords : acetylcholinesterase; abamectin; hybrid catfish; insecticideReferences
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Machado, R. Z. & Bürger, K.P. (2018). Evaluation of dot-blot test for serological diagnosis of bovine brucellosis. Brazilian Journal of Microbiology. (In press)
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and paraquat toxicity to juveniles of African catfish, Clarias gariepinus, Pakistan Journal of Zoology, 49 (1), 183-190.
Kushwaha, S., Anerao, I., Rajput, S., Bhagriya, P. & Roy, H. (2020). Evaluation of abamectin induced hepatotoxicity
in Oreochromis mossambicus. Cogent Biology, 6(1), 1-14.
Ladipo M. K., Doherty V. F. & Oyebadejo S. A. (2011). Acute Toxicity, Behavioural changes and histopathological
effect of paraquat dichloride on tissues of catfish (Clarias Gariepinus), International Journal of Biology, 3(2), 67-74.
Novellia, A., Vieiraa, B. H., Cordeirob, D., Cappelinib, L.T.D., Vieirab, E. M. & Espíndola, E. L.G. (2011). Lethal
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Thanomsit, C. & Ocharoen, Y. (2016). Fate and Toxicity of Abamectin in exposed fish. RMUTI Journal, 9 (3), 215-
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Thanomsit, C., Saowakoon, K. & Nanthanawat, P. (2016). Mortality rate and histological alterations of prawn (Macrobrachium rosenbergii) exposed to abamectin. Journal of Fisheries Technology Research, 10 (2), 23-37. (in Thai)
Thanomsit, C., Ocharoen, Y. & Nanthanawat, P. (2016). Behavior, Survival rate, behavior and histological
alterations in Nile tilapia (Orechromis niloticus-mossambicus) after exposed to abamectin. Naresuan University Journal: Science and Technology, 24(3), 65-74.
Thanomsit, C., Maprajuab, A., Prasartkaew, W., Ocharoen, Y., Wattakornsiri, A., Nanuam, J. & Nanthanawat, P.
(2017). Application of Acetylcholinesterase as biomarker for pesticide exposure to reduce health risk in consuming Pond snail and Golden apple snail, in: Inovation and Technology Conference 2017. pp. 221–227.
Thanomsit, C., Maprajub, A., Saowakoon, S., Prasatkaew, W., Ocharore, Y. Wattanakornsiri, A., Nanuam, J. & Nanthanawat, P. (2018). Acetylcholinesterase (AChE): Potential Biomarker for Evaluating Pesticide Exposure on Egg and Tissue of Golden Apple Snail (Pomacea Canaliculata) from Huai-Saneng Reservoir, Surin Province, Thailand. Thai Journal of Agricultural Science, 51 (3), 104-117.
Thanomsit, C., Saowakoon, K., Wattanakornsiri, A., Khongchareonporn, N., Nanuam, J., Prasatkaew, W., &
Nanthanawat, P. (2020a) Acetylcholinesterase (AChE) Polyclonal Antibody from Hybrid catfish (C. macrocephalus × C. gariepinus): Specification, Sensitivity and Cross Reactivity. Biochemistry and Physiology Part C: Toxicology & Pharmacology, 108837.
Thanomsit, C., Saowakoon, S., Wattanakornsiri, A., Nanuam, J., Prasatkaew, W., Nanthanawate, P., Monkolvai, P.
and Chalorcharoenying, W. 2020b. Glyphosate (Roundup): Fate, Toxicity Assessment and Adverse Effect on Aquatic Environment. 28(1), Naresuan University Journal: Science and Technology, 65-81.
Thanomsit, C., Kiatprasert, P., Prasatkaew, W., Khongchareonporn, N. & Nanthanawat, P. (2021).
Acetylcholinesterase (AChE) monoclonal antibody generation and validation for use as a biomarker of glyphosate-based herbicide exposure in commercial freshwater fish. Biochemistry and Physiology Part C: Toxicology & Pharmacology, 108956.
Tisler, T. (2006). Abamectin in the aquatic environment. Journal of Ecotoxcology, 15, 495 – 502.
Walker, C. H., Hopkin, S.P., Sibly, R. M., & Peakall, D. B. (2006). Principle of Ecotoxicology. Taylor & Francis:
USA.
You, H., Gobert, G.N., Du, X., Pali, G., Cai, P., Jones, M. K. & McManus, D.P. (2016). Functional characterisation of Schistosoma japonicum acetylcholinesterase. Parasit Vectors, 9 (328), 1615-1621.
(2007). Enzymatic inhibition and morphological changes in Hoplias malabaricus from dietary exposure to lead(II) or methylmercury. Ecotoxicology and Environmental Safety, 67, 82–88.
Akinsorotan, O. A., Morgan, P. I. & Izah, S. C. (2019). Behavioral response and acute toxicity of fingerlings of African Cat Fish, Clarias Gariepinus exposed to paraquat dichloride. Journal of plant and animal ecology, 1(3), 1-13.
Ellman, G.L., Courtney, K.D., Andres, V. & Featherstone, R. M., (1961). A new and rapid colorimetric determination
of acetylcholinesterase activity. Biochemical Pharmacology, 7, 88–95.
Bastos, C. R., Mathias,L. A., Gomes-Jusi, M.M., Ferreira dos Santos, R., Pinheiro da Silva, G.C., André, M.R.,
Machado, R. Z. & Bürger, K.P. (2018). Evaluation of dot-blot test for serological diagnosis of bovine brucellosis. Brazilian Journal of Microbiology. (In press)
Cantawon, N., Nuankaew, C., Khanchanasal, P., Saleethong, P., Kangpanich, C., Nanthanawat, P. & Thanomsit,
c. (2021). Adverse Effect of CdCl2 on Hatching Rate of Egg’s Golden Apple Snail (Pomacea canaliculata)
and Application Acetylcholinesterase (AChE) as Biomarker of Exposure. Burapha Science Journal, 26(1), 488-509.
Isaac, A .O., Joshua, O. S. and Jehu, A. (2017). Behavioural and some physiological assessment of glyphosate
and paraquat toxicity to juveniles of African catfish, Clarias gariepinus, Pakistan Journal of Zoology, 49 (1), 183-190.
Kushwaha, S., Anerao, I., Rajput, S., Bhagriya, P. & Roy, H. (2020). Evaluation of abamectin induced hepatotoxicity
in Oreochromis mossambicus. Cogent Biology, 6(1), 1-14.
Ladipo M. K., Doherty V. F. & Oyebadejo S. A. (2011). Acute Toxicity, Behavioural changes and histopathological
effect of paraquat dichloride on tissues of catfish (Clarias Gariepinus), International Journal of Biology, 3(2), 67-74.
Novellia, A., Vieiraa, B. H., Cordeirob, D., Cappelinib, L.T.D., Vieirab, E. M. & Espíndola, E. L.G. (2011). Lethal
effects of abamectin on the aquatic organisms Daphnia similis, Chironomus xanthus and Danio rerio,
Chemosphere, 86(1), 36-40.
Nuankaew, C., Ngamsnae, P., Saowakoon, S., Nanthanawat, P., & Thanomsit, C. 2020. Toxicity of Paraquat on
Physiology, Behavior and Acetylcholinesterase (AChE) Expression in Nile tilapia (Oreochromis niloticus),
in: The 4th National Conference on Innovation for Learning and Invention 2020. pp. A16-A28.
Nwani, C. D., Ifo, C.T., Nwamba, H. O., Ejere, V. C., Onyishi, G.C., Oluah, S. N., Ikwuagwu, O. E. & Odo, G. E. (2015). Oxidative stress and biochemical responses in the tissues of African catfish Clarias gariepinus juvenile following exposure to primextra herbicide. Drug and chemical toxicology, 38(3), 278–285.
Peter, L.D., Doyottea, A., Mitchelmorea, C.L., McEvoy, J. & Livingstonea, D.R. (2001). Seasonal variation and estradiol-dependent elevation of Thames estuary eel Anguilla anguilla plasma vitellogenin levels and comparisons with other United Kingdom estuaries. The Science of the Total Environment, 279, 137–150.
Rupprecht, K.R., Nair, R.K., Harwick, L.C., Grote, J., Beligere, G.S., Rege, S.D., Chen, Y.Y., Lin, Z., & Fishpaugh, J.R. (2010). Development of a dot-blot assay for screening monoclonal antibodies to low molecular-mass drugs. Analytical Biochemistry, 407, 160–164.
Thanomsit, C. & Ocharoen, Y. (2016). Fate and Toxicity of Abamectin in exposed fish. RMUTI Journal, 9 (3), 215-
226.
Thanomsit, C., Saowakoon, K. & Nanthanawat, P. (2016). Mortality rate and histological alterations of prawn (Macrobrachium rosenbergii) exposed to abamectin. Journal of Fisheries Technology Research, 10 (2), 23-37. (in Thai)
Thanomsit, C., Ocharoen, Y. & Nanthanawat, P. (2016). Behavior, Survival rate, behavior and histological
alterations in Nile tilapia (Orechromis niloticus-mossambicus) after exposed to abamectin. Naresuan University Journal: Science and Technology, 24(3), 65-74.
Thanomsit, C., Maprajuab, A., Prasartkaew, W., Ocharoen, Y., Wattakornsiri, A., Nanuam, J. & Nanthanawat, P.
(2017). Application of Acetylcholinesterase as biomarker for pesticide exposure to reduce health risk in consuming Pond snail and Golden apple snail, in: Inovation and Technology Conference 2017. pp. 221–227.
Thanomsit, C., Maprajub, A., Saowakoon, S., Prasatkaew, W., Ocharore, Y. Wattanakornsiri, A., Nanuam, J. & Nanthanawat, P. (2018). Acetylcholinesterase (AChE): Potential Biomarker for Evaluating Pesticide Exposure on Egg and Tissue of Golden Apple Snail (Pomacea Canaliculata) from Huai-Saneng Reservoir, Surin Province, Thailand. Thai Journal of Agricultural Science, 51 (3), 104-117.
Thanomsit, C., Saowakoon, K., Wattanakornsiri, A., Khongchareonporn, N., Nanuam, J., Prasatkaew, W., &
Nanthanawat, P. (2020a) Acetylcholinesterase (AChE) Polyclonal Antibody from Hybrid catfish (C. macrocephalus × C. gariepinus): Specification, Sensitivity and Cross Reactivity. Biochemistry and Physiology Part C: Toxicology & Pharmacology, 108837.
Thanomsit, C., Saowakoon, S., Wattanakornsiri, A., Nanuam, J., Prasatkaew, W., Nanthanawate, P., Monkolvai, P.
and Chalorcharoenying, W. 2020b. Glyphosate (Roundup): Fate, Toxicity Assessment and Adverse Effect on Aquatic Environment. 28(1), Naresuan University Journal: Science and Technology, 65-81.
Thanomsit, C., Kiatprasert, P., Prasatkaew, W., Khongchareonporn, N. & Nanthanawat, P. (2021).
Acetylcholinesterase (AChE) monoclonal antibody generation and validation for use as a biomarker of glyphosate-based herbicide exposure in commercial freshwater fish. Biochemistry and Physiology Part C: Toxicology & Pharmacology, 108956.
Tisler, T. (2006). Abamectin in the aquatic environment. Journal of Ecotoxcology, 15, 495 – 502.
Walker, C. H., Hopkin, S.P., Sibly, R. M., & Peakall, D. B. (2006). Principle of Ecotoxicology. Taylor & Francis:
USA.
You, H., Gobert, G.N., Du, X., Pali, G., Cai, P., Jones, M. K. & McManus, D.P. (2016). Functional characterisation of Schistosoma japonicum acetylcholinesterase. Parasit Vectors, 9 (328), 1615-1621.
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2022-06-01
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