Up-Scaling Process of Protease Production for Applying in the Manufacturing of Allergic Protein – Free Medical Glove
Abstract
Para rubber (Hevea brasiliensis) is an important economic crop of Thailand. However, natural Para rubber latex (NPRL) contains several allergic proteins and remained in medical devices which made from NPRL. Patients and medical staff are harmed by these allergens. Our research group has found that protease from Bacillus sp. bacterium is able to eliminate these allergic proteins in NPRL. However, large quantity of protease is required for using in the industrial scale. This research was subsequently aimed to study the optimal conditions for protease production by Bacillus subtilis MR10 in the 20 L fermenter and the possibility of medical glove production by allergic protein-free NPRL. It was found that the optimal condition for protease production was cultivation at 28-30 oC, aeration rate at 1.0 kg/cm2 and agitation rate at 150 rpm, leading to 1.21 unit/ml-hr of protease productivity. The obtained protease could eliminate allergic proteins, i.e. Hev b1 and Hev b2, when dot bot technique was carried out. The medical gloves produced from allergic protein free-NPRL exhibited similar thickness, color and surface to those from NPRL, however protease decreased tensile strength of gloves. Therefore, the formula development and optimization for vulcanization process need to be investigated prior application in allergic protein-free glove manufacturing. Keywords : protease ; Bacillus sp. ; up-scaling process ; allergic protein ; latex medical gloveReferences
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Chantawannakul, P., Oncharoen, A., Klanbut, K., Chukeatirote., & Lumyong, S. (2002). Characterization of
protease of Bacillus subtilis strain 38 isolated from traditionally fermented soybean in northern
Thailand. ScienceAsia, 28, 241-245.
Gouda, M.K. (2006). Optimization and purification of alkaline protease produced by marine Bacillus sp. MIG
newly isolated from eastern harbor of Alexandria. Polish Journal of Microbiology, 55, 119-126.
Na Ranong, N., & Kajornchaiyakul, V. (1996). Decrement of water soluble protein in natural rubber products.
Para Rubber Bulletin, 16(2), 81-90. (in Thai)
Nanti, S., Wongputtisin, P., Sakulsingharoj, C., Klongklaew, A., & Chomsri, N. (2014). Removal of allergenic
protein in natural rubber latex using protease from Bacillus sp. Food and Applied Bioscience Journal, 2, 216-223.
Perrella, F.W., & Gaspari, A.A. (2002). Natural rubber latex protein reduction with an emphasis on enzyme treatment. Methods, 27, 77-86.
Roychoudhury, S., Parulekar, S.J., & Weigand, W.A. (1988). Cell growth and -amylase production
characteristics of Bacillus amyloliquefaciens. Biotechnology and Bioengineering, 33, 197-206.
Sussman, G.L., Beezhold, D.H., & Liss, G. (2002). Latex allergy: historical perspective. Methods, 27, 3-9.
Wongputtisin, P., Khanongnuch, C., Khongbantad, W., Niamsup, P., & Lumyong, S. (2012). Screening and
selection of Bacillus spp. for fermented corticate soybean meal production. Journal of Applied
Microbiology, 113, 798-806.
Yeang, H.-Y. (2004). Natural rubber latex allergen: new developments. Current Opinion in Allergy and Clinical
Immunology, 4, 99-104.
Yeang, H.Y., Arif, S.R.M., Yudof, F., & Sunderasan, E. (2002). Allergenic proteins of natural rubber latex.
Methods, 27, 32-45.
Xiang, Q, Xia, K., Dai, L., Kang, G., Li, Y., Nie, Z., Duan, C., & Zeng, R. (2012). Proteome analysis of the
large and the small rubber particles of Hevea brasilliensis using 2D-DIGE. Plant Physiology and
Biochemistry, 60, 207-213
the production of thermo-stable organic solvent-tolerant protease from a newly isolated halo tolerant
Bacillus subtilis strain R. Microbial Cell Factories, 8, 1-9.
Chantawannakul, P., Oncharoen, A., Klanbut, K., Chukeatirote., & Lumyong, S. (2002). Characterization of
protease of Bacillus subtilis strain 38 isolated from traditionally fermented soybean in northern
Thailand. ScienceAsia, 28, 241-245.
Gouda, M.K. (2006). Optimization and purification of alkaline protease produced by marine Bacillus sp. MIG
newly isolated from eastern harbor of Alexandria. Polish Journal of Microbiology, 55, 119-126.
Na Ranong, N., & Kajornchaiyakul, V. (1996). Decrement of water soluble protein in natural rubber products.
Para Rubber Bulletin, 16(2), 81-90. (in Thai)
Nanti, S., Wongputtisin, P., Sakulsingharoj, C., Klongklaew, A., & Chomsri, N. (2014). Removal of allergenic
protein in natural rubber latex using protease from Bacillus sp. Food and Applied Bioscience Journal, 2, 216-223.
Perrella, F.W., & Gaspari, A.A. (2002). Natural rubber latex protein reduction with an emphasis on enzyme treatment. Methods, 27, 77-86.
Roychoudhury, S., Parulekar, S.J., & Weigand, W.A. (1988). Cell growth and -amylase production
characteristics of Bacillus amyloliquefaciens. Biotechnology and Bioengineering, 33, 197-206.
Sussman, G.L., Beezhold, D.H., & Liss, G. (2002). Latex allergy: historical perspective. Methods, 27, 3-9.
Wongputtisin, P., Khanongnuch, C., Khongbantad, W., Niamsup, P., & Lumyong, S. (2012). Screening and
selection of Bacillus spp. for fermented corticate soybean meal production. Journal of Applied
Microbiology, 113, 798-806.
Yeang, H.-Y. (2004). Natural rubber latex allergen: new developments. Current Opinion in Allergy and Clinical
Immunology, 4, 99-104.
Yeang, H.Y., Arif, S.R.M., Yudof, F., & Sunderasan, E. (2002). Allergenic proteins of natural rubber latex.
Methods, 27, 32-45.
Xiang, Q, Xia, K., Dai, L., Kang, G., Li, Y., Nie, Z., Duan, C., & Zeng, R. (2012). Proteome analysis of the
large and the small rubber particles of Hevea brasilliensis using 2D-DIGE. Plant Physiology and
Biochemistry, 60, 207-213
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2021-05-05
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