Anti-Inflammatory Activity of Folk Wisdom Boiled Herb and Its Ingredient Extracts (Phyllanthus acidus (L.) Skeels, Phyllanthus amarus Schumach. & Thonn. and Pandanus amaryllifolius Roxb.) on Inhibition of Prostaglandin E2 Production and Secretion

Authors

  • Supaporn Khamchun School of Allied Health Sciences, University of Phayao, 19 Moo 2 Tambon Maeka Amphur Muang Phayao 56000
  • Pimporn Wongrad

Abstract

Thai traditional herb had been applied as an alternative medicine extensively in various area including Si Toi, Mae Chai, Phayao province that lastingly inherited the alleviation of disease severity with boiled herb consisted Phyllanthus acidus (L.) Skeels, Phyllanthus amarus Schumach. & Thonn. and Pandanus amaryllifolius Roxb. in diabetes mellitus patients. However, the severity of this disease was revealed the intimate relation with inflammatory process in human body. The present study thus purposed the effect of abovementioned folk wisdom boiled herb and its gradient extracts on anti-inflammation through the reduction of cyclooxygenase-2 (COX-2) activity and prostaglandin E2 (PGE2) secretion, respectively. RAW264.7 macrophage cell line was treated with folk wisdom boiled herb and its gradient extracts prior induction by lipopolysaccharide (LPS). The cytotoxic and anti-inflammatory effect of these extracts were subsequently evaluated by trypan blue exclusion, COX activity and PGE2 enzyme-linked immunosorbent assay (ELISA). Our data showed the non-toxicity of folk wisdom boiled herb and its gradient extracts on macrophage cell line. Moreover, all concentrations (100, 200 and 400 µg/ml) of folk wisdom boiled herb, Phyllanthus niruri and Phyllanthus acidus at concentration of 200 and 400 µg/ml significantly inhibit PGE2 secretion and COX-2 activity in macrophage cell line with dose dependence manner (p<0.05). Accordingly, this folk wisdom boiled herb had inhibitory effect on inflammation, which derived from Phyllanthus niruri and Phyllanthus acidus, respectively. The bioactive compounds in these extracts may be explored for the further development into community health product. Keywords :  boiled herb ;  Phyllanthus niruri ; Pandanus amaryllifolius ; Inflammation ; Prostaglandin E2

Author Biography

Supaporn Khamchun, School of Allied Health Sciences, University of Phayao, 19 Moo 2 Tambon Maeka Amphur Muang Phayao 56000

Department of Medical Technology

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Published

2021-05-17