Molecular Characterization of Intergeneric hybrids between Spathoglottis plicata Bl. and Arundina graminifolia (D. Don) Hochr. by PCR-RFLP

Abstract

Molecular characterization by polymerase chain reaction - restriction fragment length polymorphism (PCR-RFLP) in nrITS region of intergeneric hybrids between Spathoglottis plicata Bl. and Arundina graminifolia (D. Don) Hochr. was investigated. The intergeneric seeding originated from deposit pollination together with tissue culture technique. PCR-RFLP profiles were generated using seven restriction enzymes (REs). Two REs, AluI and TaqI, were restricted polymorphic bands profiling whereas the others five gave monomorphic. The studies showed that probability of the hybrids from the maternal as S. plicata and A. graminifolia was 9.62% and 8.33%, respectively. However, the comparison of seed germination and seedling development with self-pollination and true intergeneric hybrids showed that there was failure in case of S. plicata as mother in deposit pollination while A. graminifolia is a suitable mother.