Molecularly Imprinted Polymer for Separation of Gallic Acid and Ellagic Acid

Abstract

Molecularly imprinted polymers stationary phase was prepared for the separation of gallic acid (GA) and ellagic acid (EGA) by thermal polymerization method using benzoyl peroxide as initiator, the mixtures of dodecanol and toluene as porogens, divinylbenzene as cross-linker and styrene as functional monomer, respectively. Gallic acid and ellagic acid were determined by HPLC using GA-EGA-MIP column, 50×4.6 mm as an analytical column with the mobile phase; water-acetonitrile (5:95, v/v) adjusted to pH 3.0. The flow rate was adjusted to 0.1 mL min-1. The injection volume was adjusted to 10 µL, and the absorption was made at 280 nm. This approach method is shown to be successful for the separation and purification of gallic acid and ellagic acid from the extract of Dimocarpus longan peels.