Effect of Dietary Probiotics Lactobacillus plantarum T13 on Growth Performance and Digestive Enzymes Activity of Pacific White Shrimp (Litopenaeus vannamei)
Abstract
This study aimed to investigate the effect of dietary probiotics Lactobacillus plantarum T13 on growth performance and digestive enzyme activity of Pacific white shrimp. Two concentrations of T13 were prepared and mixed with commercial shrimp feed to give final concentrations of approximately 109 CFU g-1 (A) and 105 CFU g-1 (B). Probiotic shrimp feeds were stored at 4°C and was prepared fresh every week. P15 shrimp, L. vannamei, were acclimated for 10 days with the average initial weight of 0.015 ± 0.0016 g and were divided into three groups: control (C) and two treatments fed with probiotic shrimp feed (A and B). The experiment was conducted in triplicate for eight weeks. During the experiment, weekly water quality was monitored and managed to maintain the acceptable aquaculture conditions. In comparison to the control group, final weight, weight gain and protease activity were significantly higher in shrimp fed with probiotics (P<0.05). Significant differences (P<0.05) for feed conversion ratio (2.03±0.15) and specific growth rate (9.48±0.14) in treatment A were observed as compared with the control but not with treatment B. However, no significant differences (P>0.05) were detected for survival rate and amylase activity among all experimental groups. Our study demonstrated that probiotic L. plantarum T13 at the concentration of 109 CFU g-1 could improve growth performance and protease activity of Pacific white shrimp. Keywords: Probiotic, Pacific white shrimp, growth performance, digestive enzymesReferences
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Becerra-Dorame, M. J., Martinez-Porchas, M., Martinez-Cordova, L. R., Rivas-Vega, M. E., Lopez-Elias, J. A., & Porchas-Cornejo, M. A. (2012). Production response and digestive enzymatic activity of the Pacific white shrimp Litopenaeus vannamei (Boone, 1931) intensively pregrown in microbial heterotrophic and autotrophic-based systems. Scientific World Journal, 2012, 723654.
Buntin, N., Chanthachum, S., & Hongpattarakere, T. (2008). Screening of lactic acid bacteria from gastrointestinal tracts of marine fish for their potential use as probiotics. Songklanakarin Journal of Science and Technology, 30(Suppl.1), 141-148.
Castex, M., Chim, L., Pham, D., Lemaire, P., Wabete, N., Nicolas, J.-L., Schmidely, P., & Mariojouls, C. (2008). Probiotic P. acidilactici application in shrimp Litopenaeus stylirostris culture subject to vibriosis in New Caledonia. Aquaculture, 275(1), 182-193.
de Vries, M. C., Vaughan, E. E., Kleerebezem, M., & de Vos, W. M. (2006). Lactobacillus plantarum-survival, functional and potential probiotic properties in the human intestinal tract. International Dairy Journal, 16(9), 1018-1028.
Desriac, F., Defer, D., Bourgougnon, N., Brillet, B., Le Chevalier, P., & Fleury, Y. (2010). Bacteriocin as weapons in the marine aminal-associated bacteria warfare: inventory and potential applications as an aquaculture probiotic. Marine drugs, 8, 1153-1177.
Dobson, A., Cotter, P. D., Ross, R. P., & Hill, C. (2012). Bacteriocin production: a probiotic trait? Applied and Environmental Microbiology, 78(1), 1-6.
Dutta, T. K., Janan, M., Pahari, P. R., & Bhattacharya, T. (2006). The Effect of Temperature, pH, and Salt on Amylase in Heliodiaptomus viduus (Gurney) (Crustacea: Copepoda: Calanoida). Turkish Journal of Zoology, 30(2), 187-195.
Farzanfar, A. (2006). The use of probiotics in shrimp aquaculture. FEMS Immunol Med Microbiol, 48(2), 149-158.
Flegel, T. W. (2006). Detection of major penaeid shrimp viruses in Asia, a historical perspective with emphasis on Thailand. Aquaculture, 258(1-4), 1-33.
Gillor, O., Etzion, A., & Riley, M. A. (2008). The dual role of bacteriocins as anti- and probiotics. Applied Microbiology and Biotechnology, 81(4), 591-606.
Guo, J.-J., Liu, K.-F., Cheng, S.-H., Chang, C.-I., Lay, J.-J., Hsu, Y.-O., Yang, J.-Y., & Chen, T.-I. (2009). Selection of probiotic bacteria for use in shrimp larviculture. Aquaculture Research, 40, 609-618.
Kewcharoen, W., & Srisapoome, P. (2019). Probiotic effects of Bacillus spp. from Pacific white shrimp (Litopenaeus vannamei) on water quality and shrimp growth, immune responses, and resistance to Vibrio parahaemolyticus (AHPND strains). Fish & Shellfish Immunology, 94, 175-189.
Lebel, L., Mungkung, R., Gheewala, S. H., & Lebel, P. (2010). Innovation cycles, niches and sustainability in the shrimp aquaculture industry in Thailand. Environmental Science & Policy, 13(4), 291-302.
Luangthuvapranit, C., & Srichai, N. (2007). Evaluation of self-prepared test kit for outdoor dissolved oxygen determination. Songklanakarin Journal of Science and Technology, 29(1), 100-108.
Luis-Villasenor, I. E., Castellanos-Cervantes, T., Gomez-Gil, B., Carrillo-Garcia, A. E., Campa-Cordova, A. I., & Ascencio, F. (2013). Probiotics in the intestinal tract of juvenile whiteleg shrimp Litopenaeus vannamei: modulation of the bacterial community. World Journal of Microbiology and Biotechnology, 29(2), 257-265.
Luis-Villasenor, I. E., Macis-Rodriguez, M. E., Gomez-Gil, B., Ascencio-Valle, F., & Campa-Cordova, A. I. (2011). Beneficial effects of four Bacillus strains on the larval cultivation of Litopenaeus vannamei. Aquaculture, 321(1-2), 136-144.
Martinez Cruz, P., Ibanez, A. L., Monroy Hermosillo, O. A., & Ramirez Saad, H. C. (2012). Use of probiotics in aquaculture. ISRN microbiology, 2012, 916845.
Nguyen, T. T. G., Nguyen, T. C., Leelakriangsak, M., Pham, T. T., Pham, Q. H., Lueangthuwapranit, C., & Nguyen, V. D. (2018). Promotion of Lactobacillus plantarum on growth and resistance against acute hepatopancreatic necrosis disease pathogens in white-leg shrimp (Litopenaeus vannamei). The Thai Journal of Veterinary Medicine, 48(1), 19-28.
Nguyen, V. D., Pham, T. T., Nguyen, T. H., Nguyen, T. T., & Hoj, L. (2014a). Screening of marine bacteria with bacteriocin-like activities and probiotic potential for ornate spiny lobster (Panulirus ornatus) juveniles. Fish Shellfish Immunology, 40(1), 49-60.
Nguyen, V. D., Pham, T. T., & Pham, N. M. Q. (2014b). Two novel strains of bacteriocin-producing Lactobacillus plantarum and their application as biopreservative in chill-stored fresh cobia meat. Journal of Pure and Applied Microbiology, 8(2), 1547-1557.
Nimrat, S., Suksawat, S., Boonthai, T., & Vuthiphandchai, V. (2012). Potential Bacillus probiotics enhance bacterial numbers, water quality and growth during early development of white shrimp (Litopenaeus vannamei). Veterinary Microbiology, 159(3-4), 443-450.
Oujifard, A., Seyfabadi, J., Abedian Kenari, A., & Rezaei, M. (2012). Growth and apparent digestibility of nutrients, fatty acids and amino acids in Pacific white shrimp, Litopenaeus vannamei, fed diets with rice protein concentrate as total and partial replacement of fish meal. Aquaculture, 342-343, 56-61.
Pineiro, M., & Stanton, C. (2007). Probiotic Bacteria: Legislative Framework Requirements to Evidence Basis. The Journal of Nutrition, 137(3), 850S-853S.
Shimogaki, H., Takeuchi, K., Nishino, T., Ohdera, M., Kudo, T., Ohba, K., Iwama, M., & Trie, M. (1991). Purification and Properties of a Novel Surface-active Agent- and Alkaline-resistant Protease from Bacillus sp. Y. Agricultural and Biological Chemistry, 55(9), 2251-2258.
Thitamadee, S., Prachumwat, A., Srisala, J., Jaroenlak, P., Salachan, P. V., Sritunyalucksana, K., Flegel, T. W., & Itsathitphaisarn, O. (2016). Review of current disease threats for cultivated penaeid shrimp in Asia. Aquaculture, 452, 69-87.
Toledo, A., Frizzo, L., Signorini, M., Bossier, P., & Arenal, A. (2019). Impact of probiotics on growth performance and shrimp survival: A meta-analysis. Aquaculture, 500, 196-205.
Vieira, F. d. N., Jatobá, A., Mouriño, J. L. P., Vieira, E. A., Soares, M., da Silva, B. C., Seiffert, W. Q., Martins, M. L., & Vinatea, L. A. (2013). In vitro selection of bacteria with potential for use as probiotics in marine shrimp culture. Pesquisa Agropecuaria Brasileira, 48(8), 998-1004.
Wang, Y.-C., Hu, S.-Y., Chiu, C.-S., & Liu, C.-H. (2019). Multiple-strain probiotics appear to be more effective in improving the growth performance and health status of white shrimp, Litopenaeus vannamei, than single probiotic strains. Fish & Shellfish Immunology, 84, 1050-1058.
Wang, Y.-B., Li, J.-R., & Lin, J. (2008). Probiotics in aquaculture Challenges and outlook. Aquaculture, 281, 1-4.
Zokaeifar, H., Balcazar, J. L., Saad, C. R., Kamarudin, M. S., Sijam, K., Arshad, A., & Nejat, N. (2012). Effects of Bacillus subtilis on the growth performance, digestive enzymes, immune gene expression and disease resistance of white shrimp, Litopenaeus vannamei. Fish & Shellfish Immunology, 33(4), 683-689.
Zuo, Z.-H., Shang, B.-J., Shao, Y.-C., Li, W.-Y., & Sun, J.-S. (2019). Screening of intestinal probiotics and the effects of feeding probiotics on the growth, immune, digestive enzyme activity and intestinal flora of Litopenaeus vannamei. Fish & Shellfish Immunology, 86, 160-168.
Becerra-Dorame, M. J., Martinez-Porchas, M., Martinez-Cordova, L. R., Rivas-Vega, M. E., Lopez-Elias, J. A., & Porchas-Cornejo, M. A. (2012). Production response and digestive enzymatic activity of the Pacific white shrimp Litopenaeus vannamei (Boone, 1931) intensively pregrown in microbial heterotrophic and autotrophic-based systems. Scientific World Journal, 2012, 723654.
Buntin, N., Chanthachum, S., & Hongpattarakere, T. (2008). Screening of lactic acid bacteria from gastrointestinal tracts of marine fish for their potential use as probiotics. Songklanakarin Journal of Science and Technology, 30(Suppl.1), 141-148.
Castex, M., Chim, L., Pham, D., Lemaire, P., Wabete, N., Nicolas, J.-L., Schmidely, P., & Mariojouls, C. (2008). Probiotic P. acidilactici application in shrimp Litopenaeus stylirostris culture subject to vibriosis in New Caledonia. Aquaculture, 275(1), 182-193.
de Vries, M. C., Vaughan, E. E., Kleerebezem, M., & de Vos, W. M. (2006). Lactobacillus plantarum-survival, functional and potential probiotic properties in the human intestinal tract. International Dairy Journal, 16(9), 1018-1028.
Desriac, F., Defer, D., Bourgougnon, N., Brillet, B., Le Chevalier, P., & Fleury, Y. (2010). Bacteriocin as weapons in the marine aminal-associated bacteria warfare: inventory and potential applications as an aquaculture probiotic. Marine drugs, 8, 1153-1177.
Dobson, A., Cotter, P. D., Ross, R. P., & Hill, C. (2012). Bacteriocin production: a probiotic trait? Applied and Environmental Microbiology, 78(1), 1-6.
Dutta, T. K., Janan, M., Pahari, P. R., & Bhattacharya, T. (2006). The Effect of Temperature, pH, and Salt on Amylase in Heliodiaptomus viduus (Gurney) (Crustacea: Copepoda: Calanoida). Turkish Journal of Zoology, 30(2), 187-195.
Farzanfar, A. (2006). The use of probiotics in shrimp aquaculture. FEMS Immunol Med Microbiol, 48(2), 149-158.
Flegel, T. W. (2006). Detection of major penaeid shrimp viruses in Asia, a historical perspective with emphasis on Thailand. Aquaculture, 258(1-4), 1-33.
Gillor, O., Etzion, A., & Riley, M. A. (2008). The dual role of bacteriocins as anti- and probiotics. Applied Microbiology and Biotechnology, 81(4), 591-606.
Guo, J.-J., Liu, K.-F., Cheng, S.-H., Chang, C.-I., Lay, J.-J., Hsu, Y.-O., Yang, J.-Y., & Chen, T.-I. (2009). Selection of probiotic bacteria for use in shrimp larviculture. Aquaculture Research, 40, 609-618.
Kewcharoen, W., & Srisapoome, P. (2019). Probiotic effects of Bacillus spp. from Pacific white shrimp (Litopenaeus vannamei) on water quality and shrimp growth, immune responses, and resistance to Vibrio parahaemolyticus (AHPND strains). Fish & Shellfish Immunology, 94, 175-189.
Lebel, L., Mungkung, R., Gheewala, S. H., & Lebel, P. (2010). Innovation cycles, niches and sustainability in the shrimp aquaculture industry in Thailand. Environmental Science & Policy, 13(4), 291-302.
Luangthuvapranit, C., & Srichai, N. (2007). Evaluation of self-prepared test kit for outdoor dissolved oxygen determination. Songklanakarin Journal of Science and Technology, 29(1), 100-108.
Luis-Villasenor, I. E., Castellanos-Cervantes, T., Gomez-Gil, B., Carrillo-Garcia, A. E., Campa-Cordova, A. I., & Ascencio, F. (2013). Probiotics in the intestinal tract of juvenile whiteleg shrimp Litopenaeus vannamei: modulation of the bacterial community. World Journal of Microbiology and Biotechnology, 29(2), 257-265.
Luis-Villasenor, I. E., Macis-Rodriguez, M. E., Gomez-Gil, B., Ascencio-Valle, F., & Campa-Cordova, A. I. (2011). Beneficial effects of four Bacillus strains on the larval cultivation of Litopenaeus vannamei. Aquaculture, 321(1-2), 136-144.
Martinez Cruz, P., Ibanez, A. L., Monroy Hermosillo, O. A., & Ramirez Saad, H. C. (2012). Use of probiotics in aquaculture. ISRN microbiology, 2012, 916845.
Nguyen, T. T. G., Nguyen, T. C., Leelakriangsak, M., Pham, T. T., Pham, Q. H., Lueangthuwapranit, C., & Nguyen, V. D. (2018). Promotion of Lactobacillus plantarum on growth and resistance against acute hepatopancreatic necrosis disease pathogens in white-leg shrimp (Litopenaeus vannamei). The Thai Journal of Veterinary Medicine, 48(1), 19-28.
Nguyen, V. D., Pham, T. T., Nguyen, T. H., Nguyen, T. T., & Hoj, L. (2014a). Screening of marine bacteria with bacteriocin-like activities and probiotic potential for ornate spiny lobster (Panulirus ornatus) juveniles. Fish Shellfish Immunology, 40(1), 49-60.
Nguyen, V. D., Pham, T. T., & Pham, N. M. Q. (2014b). Two novel strains of bacteriocin-producing Lactobacillus plantarum and their application as biopreservative in chill-stored fresh cobia meat. Journal of Pure and Applied Microbiology, 8(2), 1547-1557.
Nimrat, S., Suksawat, S., Boonthai, T., & Vuthiphandchai, V. (2012). Potential Bacillus probiotics enhance bacterial numbers, water quality and growth during early development of white shrimp (Litopenaeus vannamei). Veterinary Microbiology, 159(3-4), 443-450.
Oujifard, A., Seyfabadi, J., Abedian Kenari, A., & Rezaei, M. (2012). Growth and apparent digestibility of nutrients, fatty acids and amino acids in Pacific white shrimp, Litopenaeus vannamei, fed diets with rice protein concentrate as total and partial replacement of fish meal. Aquaculture, 342-343, 56-61.
Pineiro, M., & Stanton, C. (2007). Probiotic Bacteria: Legislative Framework Requirements to Evidence Basis. The Journal of Nutrition, 137(3), 850S-853S.
Shimogaki, H., Takeuchi, K., Nishino, T., Ohdera, M., Kudo, T., Ohba, K., Iwama, M., & Trie, M. (1991). Purification and Properties of a Novel Surface-active Agent- and Alkaline-resistant Protease from Bacillus sp. Y. Agricultural and Biological Chemistry, 55(9), 2251-2258.
Thitamadee, S., Prachumwat, A., Srisala, J., Jaroenlak, P., Salachan, P. V., Sritunyalucksana, K., Flegel, T. W., & Itsathitphaisarn, O. (2016). Review of current disease threats for cultivated penaeid shrimp in Asia. Aquaculture, 452, 69-87.
Toledo, A., Frizzo, L., Signorini, M., Bossier, P., & Arenal, A. (2019). Impact of probiotics on growth performance and shrimp survival: A meta-analysis. Aquaculture, 500, 196-205.
Vieira, F. d. N., Jatobá, A., Mouriño, J. L. P., Vieira, E. A., Soares, M., da Silva, B. C., Seiffert, W. Q., Martins, M. L., & Vinatea, L. A. (2013). In vitro selection of bacteria with potential for use as probiotics in marine shrimp culture. Pesquisa Agropecuaria Brasileira, 48(8), 998-1004.
Wang, Y.-C., Hu, S.-Y., Chiu, C.-S., & Liu, C.-H. (2019). Multiple-strain probiotics appear to be more effective in improving the growth performance and health status of white shrimp, Litopenaeus vannamei, than single probiotic strains. Fish & Shellfish Immunology, 84, 1050-1058.
Wang, Y.-B., Li, J.-R., & Lin, J. (2008). Probiotics in aquaculture Challenges and outlook. Aquaculture, 281, 1-4.
Zokaeifar, H., Balcazar, J. L., Saad, C. R., Kamarudin, M. S., Sijam, K., Arshad, A., & Nejat, N. (2012). Effects of Bacillus subtilis on the growth performance, digestive enzymes, immune gene expression and disease resistance of white shrimp, Litopenaeus vannamei. Fish & Shellfish Immunology, 33(4), 683-689.
Zuo, Z.-H., Shang, B.-J., Shao, Y.-C., Li, W.-Y., & Sun, J.-S. (2019). Screening of intestinal probiotics and the effects of feeding probiotics on the growth, immune, digestive enzyme activity and intestinal flora of Litopenaeus vannamei. Fish & Shellfish Immunology, 86, 160-168.
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2020-05-01
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