Activity of Crude Extracts from Mangrove Derived Endophytes against Fungi Contaminating on Paddy
Abstract
Preliminary screening of 23 crude extracts from culture broths of fungal endophytes isolated from mangrove plants against 8 fungi contaminated on paddy; Rhizopus spp. (KCN 4, KHP 1, KHP 2 and KMK 7) Zygomycete KCN 2, KML 8 and anamorphic fungi KCN 5, KCN 6, by disc diffusion revealed five crude extracts (21.7%) inhibited growth of at least one fungal contaminating strain on paddy. Almost all of inhibited strains are slow growing (anamorphic fungi KCN 5 and KCN 6). The ethyl acetate extracts of mycelia sterilia BUEN 887 culture broth (887EtOAc) and ethanol extracts of mycelia sterilia BUEN 938 culture broth (938EtOH) strong inhibited (IE > 30%) KCN 6 and KCN 5, respectively. When two extracts from 7 days PDB cultures of BUEN 887 and BUEN 938 were selected for inhibitory activity testing against fungi contaminating on paddy; KCN 5 and KCN 6, by soaking the paddy in the extracts for 30 minutes. The result showed that 887EtOAc was able to inhibit growth of KCN 5 and KCN 6 for at least 24 hours. Extract 938EtOH could inhibit growth of KCN 6 and KCN 5 on paddy for at least 48 and 24 hours, respectively. Keywords : crude extracts ; endophytic fungi ; mangrove plant ; paddyReferences
Ahsan, T., Chen, J., Zhao, X., Irfan, M., & Wu, Y. (2017). Extraction and identification of bioactive compounds (eicosane and double dibutyl phthalate) produced by Streoptomyces strain KX852460 for the biological control of Rhizoctonia solani AG-3 strain KX852461 to control target spot disease in tobacco leaf. AMB Express, 7, 54. doi 10.1186/s135687-017-0351-z.
Barnett, H.L., & Hunter, B.B. (2006). Illustrate genera of imperfect fungi. New York: Macmillan Publishing Company.
Dix, N.J., & Webester, J. (1995). Fungal Ecology. London: Chapman & Hall.
Durgeshlal, C., Khan, M.S., Prabhat, S.A., & Prasad, Y.A. (2019). Antifungal activity of three different ethanolic
extract against Isolates from diseased rice plant. Journal of Analytical Techniques and Research, 1: 47-63. doi: 10.26502/jatri.007
Hasan, S., Ansari, M.I., Ahmad, A., & Mishra, M. (2015). Major bioactive metabolites from marine fungi: A review. Bioinformation, 11, 176-181.
Insain, P. (2017). Bioactive compounds from endophytic fungi. EAU Heritage Journal Science and Technology, 11, 26-39.
Jayaraj, R., Rubin Jose, A.S., &. Kuruchev, V. (2018). In vitro evaluation of antifungal activity of some medicinal plants against Helminthosporium oryzae, the incitant of brown spot disease of paddy. International Journal of Advanced Research. 6, 743-746.
Justino, C.I.L., Duarte, K., Freitas, A.C., Duarte, A.C., & Rocha-Santos, T. (2014). Classical methodologies for preparation of extracts functions. In D. Barcelo (Ed.), Analysis of marine samples in search of bioactive compounds (pp. 35-57). Netherlands: Elsevier.
Keller, N.P., Tumer, G., & Bennett, J.W. (2005). Fungal secondary metabolism – from biochemistry to genomics. Nature Reviews Microbiology, 3, 937-947.
Khrueayu, D. (2013). Antimicrobial activities of bioactive compounds from endophytic fungi isolated from leaves of mangrove. Master’s Thesis, Department of microbiology Faculty of Science. Burapha University. (in Thai)
Khrueayu, D., & Pilantanapak, A. (2012). Antifungal activity of bioactive compound from endophytic fungi isolated from mangrove leaves. In Proceeding 1st Mae Fah Luang University International Conference. http://mfuic2012.mfu.ac.th/.../P-SC-B-22%20Duangnapa%20resubmitted.pdf
Lee, S.C., Muhammad, S.A., Ibrahim, M.H. & Mohamed Nor, N.M.I. (2018). Antifungal properties of Mimusops elengi seeds against paddy seed-borne fungi and selected pathogenic fungi Malaysian Journal of Microbiology, 14 (Special Issue), 152-158.
Mohapatra, D., Kumar, S., Kotwaliwale, N., & Singh, K.K. (2017). Critical factors responsible for fungi growth in stored food grains and non-chemical approaches for their control. Industrial Crops and Products, 108, 162–182.
Nakkanthong, S. (2017). Activity of crude extracts from marine derived fungal endophyte against fungi contaminating on paddy. Senior Project, Department of microbiology Faculty of Science. Burapha University. (in Thai)
Nochai, S., & Srichiwong, S. (2007). Efficacies of antagonistic fungi from rice seeds cv. KDML 105 for controlling Bakane disease in rice seeding. Journal of Agriculture, 23, 59-66. (in Thai)
Oliveira-Jr., E.N., El Gueddari, N.E., Moerschbacher, B.M., & Franco, T.T. (2012). Growth rate inhibition of phytopathogenic fungi by characterized chitosans. Brazillian Journal of Microbiology, 43, 800-809.
Pitt, J.I., Hocking, A.D., Bhudhasamai, K., Miscamble, B.F., Wheeler, K.A., & Tanboon-Ek, P. (1994). The normal mycoflora of commodities from Thailand; beans, rice small grains and other commodities. International Journal of Food Microbiology, 23, 35-53.
Reddy, K.R.N., Reddy, C.S., Abbas, H.K., Abel, C.A., & Muralidharan, K. (2008). Mycotoxigenic fungi, mycotoxins, and management of rice grains. Toxins Reviews, 27, 287-317.
Samson, R.A., Hoekstra, E.S., & Frisvad, J.C. (2004). Introduction to food and airlborne fungi. 7th Edition, Utrecht: Centraalbureau voor Schimmelcultures.
Sparrianga, R.A., & Owens, J.D. (2002). Inhibition of the tempe mould, Rhizopus oligosporus, by ammonia. Letters in Applied Microbiology, 29. https://doi.org/10.1046/j.1365-2672.1999.00591.x
Swathi, J., Narendra, K., Sowjanya, K.M., & Krishna Satya, A. (2013). Marine fungal metabolites as a rich source of bioactive compounds. African Journal of Biochemistry Research, 7, 184-196.
Tanakulpakorn, J., Pilantanapak, A., & Srivibool, R. (2012). Antifungal activity of endophytic fungi TH121 isolated from Thespesia populnea against Alternaria brassicicola. Thai Journal of Science and Technology, 1, 32-41. (in Thai)
Thai Rice Exporters Association. (2016). Thai Rice Exporters Association News. Retrieved May 22, 2018, from http://www.thairiceexporters.or.th/Press%20release/2016/TREA%20Press%20Release%20%20January%202016%20-%2029012016.pdf (in Thai)
Vaske, Y.M., & Crews, P. (2014). An update on the biomedical prospects of marine-derived small molecules with fascinating stom and stereochemical diversity. In B. Hernandez-Ledesma, & M. Herrero, (Eds.), Bioactive compounds from marine foods: plant and animal source. (pp.1-26). United States: Wiley-Blackwell.
Wisniewski, M., Wilson, C., & Hershberger, W. (2011). Characterization of inhibition of Rhizopus stolonifer germination and growth by Enterobacter cloacae. Canadian Journal of Botany 67, 2317-2323.
Wikipedia (2019). Rice. Retrived July 3, 2019, from https://en.wikipedia.org/wiki/Rice
Wongcharoen, A. (2014). Screening of endophytic fungi from rice (Oryza sativa L.) against rice pathogenic fungi. Khon Kaen Agriculture Journal, 42, 385-396. (in Thai)
Barnett, H.L., & Hunter, B.B. (2006). Illustrate genera of imperfect fungi. New York: Macmillan Publishing Company.
Dix, N.J., & Webester, J. (1995). Fungal Ecology. London: Chapman & Hall.
Durgeshlal, C., Khan, M.S., Prabhat, S.A., & Prasad, Y.A. (2019). Antifungal activity of three different ethanolic
extract against Isolates from diseased rice plant. Journal of Analytical Techniques and Research, 1: 47-63. doi: 10.26502/jatri.007
Hasan, S., Ansari, M.I., Ahmad, A., & Mishra, M. (2015). Major bioactive metabolites from marine fungi: A review. Bioinformation, 11, 176-181.
Insain, P. (2017). Bioactive compounds from endophytic fungi. EAU Heritage Journal Science and Technology, 11, 26-39.
Jayaraj, R., Rubin Jose, A.S., &. Kuruchev, V. (2018). In vitro evaluation of antifungal activity of some medicinal plants against Helminthosporium oryzae, the incitant of brown spot disease of paddy. International Journal of Advanced Research. 6, 743-746.
Justino, C.I.L., Duarte, K., Freitas, A.C., Duarte, A.C., & Rocha-Santos, T. (2014). Classical methodologies for preparation of extracts functions. In D. Barcelo (Ed.), Analysis of marine samples in search of bioactive compounds (pp. 35-57). Netherlands: Elsevier.
Keller, N.P., Tumer, G., & Bennett, J.W. (2005). Fungal secondary metabolism – from biochemistry to genomics. Nature Reviews Microbiology, 3, 937-947.
Khrueayu, D. (2013). Antimicrobial activities of bioactive compounds from endophytic fungi isolated from leaves of mangrove. Master’s Thesis, Department of microbiology Faculty of Science. Burapha University. (in Thai)
Khrueayu, D., & Pilantanapak, A. (2012). Antifungal activity of bioactive compound from endophytic fungi isolated from mangrove leaves. In Proceeding 1st Mae Fah Luang University International Conference. http://mfuic2012.mfu.ac.th/.../P-SC-B-22%20Duangnapa%20resubmitted.pdf
Lee, S.C., Muhammad, S.A., Ibrahim, M.H. & Mohamed Nor, N.M.I. (2018). Antifungal properties of Mimusops elengi seeds against paddy seed-borne fungi and selected pathogenic fungi Malaysian Journal of Microbiology, 14 (Special Issue), 152-158.
Mohapatra, D., Kumar, S., Kotwaliwale, N., & Singh, K.K. (2017). Critical factors responsible for fungi growth in stored food grains and non-chemical approaches for their control. Industrial Crops and Products, 108, 162–182.
Nakkanthong, S. (2017). Activity of crude extracts from marine derived fungal endophyte against fungi contaminating on paddy. Senior Project, Department of microbiology Faculty of Science. Burapha University. (in Thai)
Nochai, S., & Srichiwong, S. (2007). Efficacies of antagonistic fungi from rice seeds cv. KDML 105 for controlling Bakane disease in rice seeding. Journal of Agriculture, 23, 59-66. (in Thai)
Oliveira-Jr., E.N., El Gueddari, N.E., Moerschbacher, B.M., & Franco, T.T. (2012). Growth rate inhibition of phytopathogenic fungi by characterized chitosans. Brazillian Journal of Microbiology, 43, 800-809.
Pitt, J.I., Hocking, A.D., Bhudhasamai, K., Miscamble, B.F., Wheeler, K.A., & Tanboon-Ek, P. (1994). The normal mycoflora of commodities from Thailand; beans, rice small grains and other commodities. International Journal of Food Microbiology, 23, 35-53.
Reddy, K.R.N., Reddy, C.S., Abbas, H.K., Abel, C.A., & Muralidharan, K. (2008). Mycotoxigenic fungi, mycotoxins, and management of rice grains. Toxins Reviews, 27, 287-317.
Samson, R.A., Hoekstra, E.S., & Frisvad, J.C. (2004). Introduction to food and airlborne fungi. 7th Edition, Utrecht: Centraalbureau voor Schimmelcultures.
Sparrianga, R.A., & Owens, J.D. (2002). Inhibition of the tempe mould, Rhizopus oligosporus, by ammonia. Letters in Applied Microbiology, 29. https://doi.org/10.1046/j.1365-2672.1999.00591.x
Swathi, J., Narendra, K., Sowjanya, K.M., & Krishna Satya, A. (2013). Marine fungal metabolites as a rich source of bioactive compounds. African Journal of Biochemistry Research, 7, 184-196.
Tanakulpakorn, J., Pilantanapak, A., & Srivibool, R. (2012). Antifungal activity of endophytic fungi TH121 isolated from Thespesia populnea against Alternaria brassicicola. Thai Journal of Science and Technology, 1, 32-41. (in Thai)
Thai Rice Exporters Association. (2016). Thai Rice Exporters Association News. Retrieved May 22, 2018, from http://www.thairiceexporters.or.th/Press%20release/2016/TREA%20Press%20Release%20%20January%202016%20-%2029012016.pdf (in Thai)
Vaske, Y.M., & Crews, P. (2014). An update on the biomedical prospects of marine-derived small molecules with fascinating stom and stereochemical diversity. In B. Hernandez-Ledesma, & M. Herrero, (Eds.), Bioactive compounds from marine foods: plant and animal source. (pp.1-26). United States: Wiley-Blackwell.
Wisniewski, M., Wilson, C., & Hershberger, W. (2011). Characterization of inhibition of Rhizopus stolonifer germination and growth by Enterobacter cloacae. Canadian Journal of Botany 67, 2317-2323.
Wikipedia (2019). Rice. Retrived July 3, 2019, from https://en.wikipedia.org/wiki/Rice
Wongcharoen, A. (2014). Screening of endophytic fungi from rice (Oryza sativa L.) against rice pathogenic fungi. Khon Kaen Agriculture Journal, 42, 385-396. (in Thai)
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2020-09-01
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