The Optimum Conditions for Determination of Dimethylarsenate and Monomethylarsonate using Purge and Trap Gas Chromatography-Mass Spectrometry Techniques
Abstract
The study of the determination conditions of dimethylarsenate and monomethylarsonate in water were carried out using purge and trap gas chromatography-mass spectrometry techniques. The results show that the optimum conditions were purge time was 2 minutes. The Helium gas desorb flow rate was 200 ml/min. The transfer line temperature was 120 ◦C. The desorb temperature was 250◦C and the desorb time was 3 minutes. Within these optimum conditions, the limit of detection and the limit of quantification were 0.05-0.02 µL-1 and 0.17-0.05 µL-1, respectively. The precision was in the range of 2.34-19.12% (n=10).References
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solid phase extraction methods for speciation of arsenic in natural water using multivariate technique. Analytica Chimica Acta,
651, 57–63.
[2] Yoshida, T., Yamauchi, H. and Sun, G.F. (2004). Chronic health effects in people exposed to arsenic via the drinking water: dose
response relationships in review. Toxicology and Applied Pharmacology, 198, 243–252.
[3] Kazi, T. G., Arain, M. B., Baig, J. A., Jamali, M. K., Afridi, H. I., Jalbani, N., Sarfraz, R. A., Niaz, A. (2009). The correlation of arsenic
levels in drinking water with the biological samples of skin disorders. Science of The Total Environment, 407 (3), 1019–1026
[4] WHO. (1996). Arsenic Compounds Environmental Health Criteria 224 2nd ed. World Health Organisation, Geneva.
[5] Milstein, L.S., Essader, A., Pellizzari, E.D., Fernando, R.A. and Akinbo, O. (2002). Selection of suitable mobile phase for the speciation of
four arsenic compound in drinking water samples using ion-exchange chromatography coupled to inductively coupled plasma
mass spectrometry. Environment International, 28, 277-283.
[6] Rahman, M.A., Hasegawa, H. and Lim , R.p. (2012). Bioaccumulation, biotransformation and trophic transfer of arsenic in the aquatic
food chain. Environmental Research, 116, 118-135.
[7] Tuzen, M., Saygi, K.O., Karaman, I. and Soylak, M. (2010). Selective speciation and determination of inorganic arsenic in water, food
and biological samples. Food and Chemical Toxicology, 48, 41-46.
[8] Uluozlu, O.D., Tuzen, M., Mendil, D. and Soylak, M. (2010). Determinaton of As(III) and As(V) species in some natural water and food
samples by solid-phase extraction on Streptococcus pyogenes immobilized on Sepabeads SP 70 and hydride generation
atomic absorption spectrometry. Food and Chemical Toxicology, 48, 1393-1398.
[9] Nam, S.H., Oh, H.J., Min, H.S. and Lee, J.H. (2010). A study on the extraction and quantitation of total arsenic and arsenic species in
seafood by HPLC-ICP-MS. Microchemical Journal, 95, 20-24.
[10] Ronkart, S.N., Laurent, V., Carbonnelle. P., Mabon, N., Copin, A. and Barthélemy, J.P. (2007). Speciation of five arsenic species
(arsenite, arsenate, MMAA(V), DMAA(V) and AsBet) in different kind of water by HPLC-ICP-MS. Chemosphere, 66, 738-745.
[11] Anawar, H.M. (2012). Arsenic speciation in environmental samples by hydride generation and electrothermal atomic absorption
spectrometry. Talanta, 88, 30-42.
[12] AOAC international. (1993). AOAC® peer-verified method program: manual on policies and procedures. United States of America.
