Production of Monoclonal Antibody Specific to Immunoglobulin of Asian Sea Bass Lates calcarifer
Abstract
Production of monoclonal antibodies (MAbs) specific to Asian sea bass immunoglobulin (Ig) was prepared by peritoneal injection the Asian sea bass with Bovine serum albumin (BSA) first. Then the fish anti-BSA antiserum was used to precipitate with BSA by immunoprecipitation method. The Ig/BSA complex was then immunized into mice. The mouse that showed the strongest immune response against Asian sea bass Ig was used for monoclonal antibody production. After fusion of myeloma with B cells of mice, 3 hybridoma clones (4C7, 6H7 and 15C8) producing monoclonal antibody specific to heavy chain (~80 kDa) of Asian sea bass Ig were selected by dot blot and Western blot, however the MAb (4C7) showed slightly cross reaction with tilapia serum. Keywords: monoclonal antibody, Lates calcarifer, immunoglobulin, double immunodiffusion, Western blotReferences
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Bradford, M. M. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 72(1), 248-254. doi:https://doi.org/10.1016/ 0003-2697(76)90527-3
Chettri, J. K., Jaafar, R. M., Skov, J., Kania, P. W., Dalsgaard, I., & Buchmann, K. (2015). Booster immersion vaccination using diluted Yersinia ruckeri bacterin confers protection against ERM in rainbow trout. Aquaculture, 440, 1-5. doi:https://doi.org/10.1016/j.aquaculture.2015.01.027
Choudhury, M., & Pani Prasad, K. (2011). Isolation and characterization of immunoglobulin M of Asian sea bass, Lates calcarifer and its level in serum. Central European Journal of Biology, 6, 180-187. doi:10.2478/ s11535-010-0109-y
Crosbie, P. B. B., & Nowak, B. F. (2002). Production of polyclonal antisera against barramundi (Lates calcarifer Bloch) serum immunoglobulin derived from affinity columns containing mannan-binding protein or staphylococcal protein A. Aquaculture, 211(1), 49-63. doi:https://doi.org/10.1016/S0044-8486(02)00136-9
de Groof, A., Guelen, L., Deijs, M., van der Wal, Y., Miyata, M., Ng, K. S., van Grinsven, L., Simmerlink,B.,
Biermann, Y., Grisez, L., van Lent, J., de Ronde, A., Chang, S.F., Schrier, C. & van der Hoek, L. (2015).
A Novel Virus Causes Scale Drop Disease in Lates calcarifer. PLoS Pathog, 11(8), e1005074.
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Kayansamruaj, P., Dong, H. T., Nguyen, V. V., Le, H. D., Pirarat, N., & Rodkhum, C. (2015). Susceptibility of freshwater rearing Asian seabass (Lates calcarifer) to pathogenic Streptococcus iniae. Aquaculture Research, 48(2), 711-718. doi:10.1111/are.12917
Kreutz, L. C., Canova, R., Nied, C. O., Bortoluzzi, M., & Frandoloso, R. (2016). Characterization of an IgM-like immunoglobulin from silver catfish (Rhamdia quelen) serum and its use for the production of polyclonal antibodies and development of immunoassays. Pesquisa Veterinária Brasileira, 36(9), 819-825. doi:10.1590/s0100-736x2016000900005
Mashoof, S., & Criscitiello, F. M. (2016). Fish Immunoglobulins. Biology, 5(4). doi:10.3390/biology5040045
Palenzuela, O., SitjÀ-Bobadilla, A., & Alvarez-Pellitero, P. (1996). Isolation and partial characterization of serum immunoglobulins from sea bass (Dicentrarchus labrax L.) and gilthead sea bream (Sparus aurata L.). Fish & Shellfish Immunology, 6, 81–94. doi:10.1006/fsim.1996.0010
Pengsuk, C., Longyant, S., Rukpratanporn, S., Chaivisuthangkura, P., Sridulyakul, P., & Sithigorngul, P. (2010). Development of monoclonal antibodies for simple detection and differentiation of Vibrio mimicus from V. cholerae and Vibrio spp. by dot blotting. Aquaculture, 300, 17-24. doi:10.1016/j.aquaculture.2009.12.023
Smith, S. A., Gebhard, D. H., Housman, J. M., Levy, M. G., & Noga, E. J. (1993). Isolation, Purification, and Molecular-Weight Determination of Serum Immunoglobulin from Oreochromis aureus. Journal of Aquatic Animal Health, 5(1), 23-35. doi:10.1577/1548-8667(1993)005<0023:IPAMWD>2.3.CO;2
Soonthonsrima, T., Wangman, P., Sithigorngul, P., & Longyant, S. (2017). Production of Polyclonal Antibody Against Immunoglobulin of Tilapia Oreochromis niloticus. Burapha Science Journal, 22(3), 596-605.
(in Thai).
Soonthonsrima, T., Wangman, P., Chaivisuthangkura, P., Pengsuk, C., Sithigorngul, P., & Longyant, S. (2018). Generation of mouse monoclonal antibodies specific to tilapia immunoglobulin using fish immunoglobulin/BSA complex for monitoring of the immune response in Nile tilapia Oreochromis niloticus. Aquaculture Research, 50(1), 277-283. doi:10.1111/are.13894
Thipduang, T. (2012). The Concept of the Imported and Exported Seabass Control which passed theSouthern Fish Inspection station in 2007-2009. Retrieved from Department of Fisheries, Ministry of Agriculture and Cooperatives
Vesely, T., Reschova, S., Pokorová, D., Hulova, J., & Smržová, Z. (2006). Production of monoclonal antibodies against immunoglobulin heavy chain in common carp (Cyprinus carpio L.). Veterinarni Medicina, 51, doi:10.17221/5549-VETMED
Wangman, P., Siriwattanarat, R., Longyant, S., Pengsuk, C., Sithigorngul, P., & Chaivisuthangkura, P. (2016). High sensitivity immunochromatographic strip test (ICP11 strip test) for white spot syndrome virus detection using monoclonal antibodies specific to ICP11 non-structural protein. Aquaculture, 470,
25-31. doi:10.1016/j.aquaculture.2016.12.004
Yang, S., Tang, X., Sheng, X., Xing, J., & Zhan, W. (2017). Development of monoclonal antibodies against IgM of half-smooth tongue sole (Cynoglossus semilaevis) and analysis of phagocytosis of fluorescence microspheres by mIgM+ lymphocytes. Fish Shellfish Immunol, 66, 280-288. doi:10.1016/j.fsi.2017.05.019
Yang, S., Tang, X., Sheng, X., Xing, J., & Zhan, W. (2018). Development of monoclonal antibodies against IgM of sea bass (Lateolabrax japonicus) and analysis of phagocytosis by mIgM+ lymphocytes. Fish Shellfish Immunol, 78, 372-382. doi:10.1016/j.fsi.2018.04.042
Ye, J., Kaattari, I. M., Ma, C., & Kaattari, S. (2013). The teleost humoral immune response. Fish & Shellfish Immunology, 35(6), 1719-1728. doi:https://doi.org/10.1016/j.fsi.2013.10.015
Zhang, W., Tang, X. Q., Sheng, X. Z., Xing, J., & Zhan, W. B. (2017). Development and application of monoclonal antibodies against IgM of black rockfish Sebastes schlegeli. J Fish Biol, 90(4), 1668-1675. doi:10.1111/jfb.13279
Bradford, M. M. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 72(1), 248-254. doi:https://doi.org/10.1016/ 0003-2697(76)90527-3
Chettri, J. K., Jaafar, R. M., Skov, J., Kania, P. W., Dalsgaard, I., & Buchmann, K. (2015). Booster immersion vaccination using diluted Yersinia ruckeri bacterin confers protection against ERM in rainbow trout. Aquaculture, 440, 1-5. doi:https://doi.org/10.1016/j.aquaculture.2015.01.027
Choudhury, M., & Pani Prasad, K. (2011). Isolation and characterization of immunoglobulin M of Asian sea bass, Lates calcarifer and its level in serum. Central European Journal of Biology, 6, 180-187. doi:10.2478/ s11535-010-0109-y
Crosbie, P. B. B., & Nowak, B. F. (2002). Production of polyclonal antisera against barramundi (Lates calcarifer Bloch) serum immunoglobulin derived from affinity columns containing mannan-binding protein or staphylococcal protein A. Aquaculture, 211(1), 49-63. doi:https://doi.org/10.1016/S0044-8486(02)00136-9
de Groof, A., Guelen, L., Deijs, M., van der Wal, Y., Miyata, M., Ng, K. S., van Grinsven, L., Simmerlink,B.,
Biermann, Y., Grisez, L., van Lent, J., de Ronde, A., Chang, S.F., Schrier, C. & van der Hoek, L. (2015).
A Novel Virus Causes Scale Drop Disease in Lates calcarifer. PLoS Pathog, 11(8), e1005074.
doi:10.1371/journal. ppat.1005074
Kayansamruaj, P., Dong, H. T., Nguyen, V. V., Le, H. D., Pirarat, N., & Rodkhum, C. (2015). Susceptibility of freshwater rearing Asian seabass (Lates calcarifer) to pathogenic Streptococcus iniae. Aquaculture Research, 48(2), 711-718. doi:10.1111/are.12917
Kreutz, L. C., Canova, R., Nied, C. O., Bortoluzzi, M., & Frandoloso, R. (2016). Characterization of an IgM-like immunoglobulin from silver catfish (Rhamdia quelen) serum and its use for the production of polyclonal antibodies and development of immunoassays. Pesquisa Veterinária Brasileira, 36(9), 819-825. doi:10.1590/s0100-736x2016000900005
Mashoof, S., & Criscitiello, F. M. (2016). Fish Immunoglobulins. Biology, 5(4). doi:10.3390/biology5040045
Palenzuela, O., SitjÀ-Bobadilla, A., & Alvarez-Pellitero, P. (1996). Isolation and partial characterization of serum immunoglobulins from sea bass (Dicentrarchus labrax L.) and gilthead sea bream (Sparus aurata L.). Fish & Shellfish Immunology, 6, 81–94. doi:10.1006/fsim.1996.0010
Pengsuk, C., Longyant, S., Rukpratanporn, S., Chaivisuthangkura, P., Sridulyakul, P., & Sithigorngul, P. (2010). Development of monoclonal antibodies for simple detection and differentiation of Vibrio mimicus from V. cholerae and Vibrio spp. by dot blotting. Aquaculture, 300, 17-24. doi:10.1016/j.aquaculture.2009.12.023
Smith, S. A., Gebhard, D. H., Housman, J. M., Levy, M. G., & Noga, E. J. (1993). Isolation, Purification, and Molecular-Weight Determination of Serum Immunoglobulin from Oreochromis aureus. Journal of Aquatic Animal Health, 5(1), 23-35. doi:10.1577/1548-8667(1993)005<0023:IPAMWD>2.3.CO;2
Soonthonsrima, T., Wangman, P., Sithigorngul, P., & Longyant, S. (2017). Production of Polyclonal Antibody Against Immunoglobulin of Tilapia Oreochromis niloticus. Burapha Science Journal, 22(3), 596-605.
(in Thai).
Soonthonsrima, T., Wangman, P., Chaivisuthangkura, P., Pengsuk, C., Sithigorngul, P., & Longyant, S. (2018). Generation of mouse monoclonal antibodies specific to tilapia immunoglobulin using fish immunoglobulin/BSA complex for monitoring of the immune response in Nile tilapia Oreochromis niloticus. Aquaculture Research, 50(1), 277-283. doi:10.1111/are.13894
Thipduang, T. (2012). The Concept of the Imported and Exported Seabass Control which passed theSouthern Fish Inspection station in 2007-2009. Retrieved from Department of Fisheries, Ministry of Agriculture and Cooperatives
Vesely, T., Reschova, S., Pokorová, D., Hulova, J., & Smržová, Z. (2006). Production of monoclonal antibodies against immunoglobulin heavy chain in common carp (Cyprinus carpio L.). Veterinarni Medicina, 51, doi:10.17221/5549-VETMED
Wangman, P., Siriwattanarat, R., Longyant, S., Pengsuk, C., Sithigorngul, P., & Chaivisuthangkura, P. (2016). High sensitivity immunochromatographic strip test (ICP11 strip test) for white spot syndrome virus detection using monoclonal antibodies specific to ICP11 non-structural protein. Aquaculture, 470,
25-31. doi:10.1016/j.aquaculture.2016.12.004
Yang, S., Tang, X., Sheng, X., Xing, J., & Zhan, W. (2017). Development of monoclonal antibodies against IgM of half-smooth tongue sole (Cynoglossus semilaevis) and analysis of phagocytosis of fluorescence microspheres by mIgM+ lymphocytes. Fish Shellfish Immunol, 66, 280-288. doi:10.1016/j.fsi.2017.05.019
Yang, S., Tang, X., Sheng, X., Xing, J., & Zhan, W. (2018). Development of monoclonal antibodies against IgM of sea bass (Lateolabrax japonicus) and analysis of phagocytosis by mIgM+ lymphocytes. Fish Shellfish Immunol, 78, 372-382. doi:10.1016/j.fsi.2018.04.042
Ye, J., Kaattari, I. M., Ma, C., & Kaattari, S. (2013). The teleost humoral immune response. Fish & Shellfish Immunology, 35(6), 1719-1728. doi:https://doi.org/10.1016/j.fsi.2013.10.015
Zhang, W., Tang, X. Q., Sheng, X. Z., Xing, J., & Zhan, W. B. (2017). Development and application of monoclonal antibodies against IgM of black rockfish Sebastes schlegeli. J Fish Biol, 90(4), 1668-1675. doi:10.1111/jfb.13279
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2019-10-01
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