In vitro Plant Regeneration and Callus Induction from Leaf Explants of Sundews (Drosera spathulata Labill. and Drosera adelae F. Muell.)
Abstract
In vitro plant Plant regeneration and callus induction using leaf explants of two Sundew species, D. spathulata Labill. and D. adelae F. Muell. were examined on semi-solid MS media with varied strength of concentration, MS, 1/2MS, 1/3MS and 1/4MS, for 8 weeks. The result found that leaf explants of D. spathulata Labill. cultured on 1/4MS media could be induced shoot and flowering as well as the highest average number of shoot and inflorescences. While the leaf explants cultured on 1/2MS media could be induced the root production with the highest average of root number and root length. For the leaf explants of D. adelae F. Muell. cultured on 1/4MS media, they could be induce shoots and roots production with the highest average number of shoot and root. To induce callus, the leaf explants were cultured on 1/4MS medium with varied 0, 0.5, 1.0, 1.5 and 2.0 mg/l BA, for 8 weeks. The leaf explants of both species cultured on 1/4MS media with 0.5 mg/l BA gave the best development of callus with the longest diameter and the highest fresh weight of callus, while the leaf explants cultured on 1/4MS media without BA plant growth regulators could be induced shoots and root production. When new shoot were cultured on the 1/4MS media with 0, 0.5, 1.0, 1.5 and 2.0 mg/l IBA, for 6 weeks, the shoot of D. spathulata Labill. cultured on 1/4MS with 1.5 mg/l IBA had the highest number average of root. While the shoot explants of D. adelae F. Muell. culturing on 1/4MS media containing 1.0 mg/l IBA had the highest number average of root. Keywrods : callus induction, in vitro flowering, plant growth regulators, sundewsReferences
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Droesera intermedia in a single step. Biologia Plantarum, 54(2), 391-394.
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Plant Cell, Tissue and Organ Culture, 72, 95–98.
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a vulnerable and medicinally important insectivorous plant. plant Biotechnol Rep, 1, doi: 79-84. doi10.1007/s11816-007-0014-7
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a vulnerable and medicinally important insectivorous plant. Indian Journal of
Biotechnology, 7, 260-265.
Khumdinpitag, A., Krasaechai, A., & Phornsawatchai, T. (2004). In vitro propagation of pitcher plant
(Nepenthes thorelii). Journal of Agriculture, 20(1), 1-9.
Kim, K.-S., & Jang, G.-W. (2004). Micropropagation of Droseara peltata, a tuberous sundew, by
shoot tip culture. Plant Cell, Tissue and Organ Culture, 77, 211-214.
Krolicka, A., Szpitter, A., Gilgenast, E., Romanik, G., Kaminski, M., & Lojkowska, E. (2008).
Stimulation of antibacterial naphthoquinones and flavonoids accumulation in carnivorous plants grown in vitro by addition of elicitors. Enzyme and Microbial Technology, 42, 216-221.
Kumlay, A. M., & Ercisli S. (2015). Callus induction, shoot proliferation and root regeneration
of potato (Solanum tuberosum L.) stem node and leaf explants under long-day conditions. Biotechnology & Biotechnological Equipment, 29(6), 1075-1084.
Lee, L. (2008). Carnivorous plants: new ornamentals. Chronica Horticulturae, 48(4), 11-14.
Navaphanich, U. (2006). Plant tissue culture. (1) Bangkok: Ramkhamhaeng University Press. (in Thai)
Pasek, K. (1999). Sowing carnivorous plant seeds. Retrieved from http://www.bestcarnivorousplants.com
Songsri, O., Jirakiattikul, Y., Rithichai, P., & Itharat, A. (2013). Effect of auxin on In vitro shoot multiplication
and root inductionof Hua-Khao-Yen (Dioscorea birmanica Prain&Burkill). Agricultural Sci. J.,
44(2), 49-52. (in Thai)
Taweerodjanakarn, S., Kongton, K., Thongyai, K., & Samala, S. (2018). In vitro micropropagation of
Drosera burmannii Vahl. Songklanakarin Journal of Plant Science, 5(3), 18-26. (in Thai)
Techapinyawat, S. (2001). Plant Physiology. Bangkok: Publisher Kasetsart University. (in Thai)
Thaweesak, J., Seiichi, S., Hiroyuki, T., & Waraporn, P. (2011). Elicitation effect on production
of plumbagin in in vitro culture of Drosera indica L. Journal of Medicinal Plants
Research, 5(19), 4949-4953.
Tomaz, R., & Marina, D. (2010). Cytokinins and their function in developing seeds. Acta
Chimica Slovenica, 57, 617-629.
Wongchaochant, S. & Phumphothong, S. (2015). In vitro micropropagation of Venus flytrap (Dionaea
muscipula Soland. ex Ellis). In The 53rd Kasetsart University Annual Conference (pp. 590-650). Bangkok: Kasetsart University. (in Thai)
Ziaratmai, S. M., Kunert K. J., & Lall, N. (2009). Elicitation of 7-methyjuglone in Drosera capensis.
South African Journal of Botany, 75, 97-103.
Banasiuk, R., Kawiak, A., & Krolicka, A. (2012). In vitro cultures of carnivorous plants from the
Drosera and Dionaea genus for the production of biologically active secondary metabolites. BioTechnologia Journal of Biotechnology, Computational Biology and Bionanotechnology,
93(2), 87-96.
Chang, H. C., Lu, C. Y., Chen, C. C., Kuo, C. L., Tasay, H. S., & Agrawal, D. C. (2019). Plumbagin, a plant-
derived naphthoquinone production in tissue cultures of Drosera spatulata Labill. Biotechnology, 18(1), 24-31.
Charoenma, K. (2008). Can insectivorous plants handle insect-eating insects? Entomology and zoology
gazette, 26(1), 77-79. (in Thai)
Charoenwattana, P. (2014). In vitro cultures and colchicine-induced tetraploidy of Sundew (Drosera
spatulata Labill.). International Journal of Environmental and Rural Development, 5(2), 80-85.
Chuntaratin, P. (2013). Collection and production of carnivorous plants in the genus Drosera by plant tissue
culture technique. Retrieved from http://qa.bkkthon.ac.th/qa/qa56/132144099.pdf (in Thai)
Goncalves, S., & Romano, A. (2005). Micropropagation of Drosophyllum lusitanicum (Dewy pine), an
endangered West Mediterranean endemic insectivorous plant. Biodiversity and Conservation, 14,
1071-1081.
Grevenstuk, T., Coelho, N., Goncalves, S., & Romano, A. (2010). In vitro propagation of
Droesera intermedia in a single step. Biologia Plantarum, 54(2), 391-394.
Hook, I. L. I. (2001). Naphthaquinone contants of in vitro cultured plants and cell suspensions of
Dionaea muscipula and Drosera species. Plant Cell, Tissue and Organ Culture, 67, 281-285.
Ichiishi, S., Nagamitsu, T., Kondo, Y., Iwashina, T., Kondo, K., & Tagashira, N. (1999). Effects
of macro-components and sucrose in the medium on in vitro red-color pigmentation in Dionaea muscipula Ellis and Drosera spatulata Labill. Plant Biotechnology, 16(3), 235-238.
Jadczak, P., Kulpa, D., Zbrojewska, A. (2017). In Vitro micropropagation of Drosera rotundifolia. World
Scientific News, 66, 75-85.
Jala, A., & Phaitune, s. (2013). Micropropagationof Dionaea muscipula by tissue culture. Thai Journal of
Science and Technology, 2(2), 134-139. (in Thai)
Jang, G.W., K.S. Kim and R.D. Park. (2003). Micropropagation of Venus fly trap by shoot culture. Journal of
Plant Cell, Tissue and Organ Culture, 72, 95–98.
Jayaram, K., & Prasad, M. N. V. (2007). Rapid in vitro multiplication of Drosera indica L.:
a vulnerable and medicinally important insectivorous plant. plant Biotechnol Rep, 1, doi: 79-84. doi10.1007/s11816-007-0014-7
Jayaram, K., & Prasad, M. N. V. (2008). Rapid in vitro multiplication of Drosera burmanii Vahl.:
a vulnerable and medicinally important insectivorous plant. Indian Journal of
Biotechnology, 7, 260-265.
Khumdinpitag, A., Krasaechai, A., & Phornsawatchai, T. (2004). In vitro propagation of pitcher plant
(Nepenthes thorelii). Journal of Agriculture, 20(1), 1-9.
Kim, K.-S., & Jang, G.-W. (2004). Micropropagation of Droseara peltata, a tuberous sundew, by
shoot tip culture. Plant Cell, Tissue and Organ Culture, 77, 211-214.
Krolicka, A., Szpitter, A., Gilgenast, E., Romanik, G., Kaminski, M., & Lojkowska, E. (2008).
Stimulation of antibacterial naphthoquinones and flavonoids accumulation in carnivorous plants grown in vitro by addition of elicitors. Enzyme and Microbial Technology, 42, 216-221.
Kumlay, A. M., & Ercisli S. (2015). Callus induction, shoot proliferation and root regeneration
of potato (Solanum tuberosum L.) stem node and leaf explants under long-day conditions. Biotechnology & Biotechnological Equipment, 29(6), 1075-1084.
Lee, L. (2008). Carnivorous plants: new ornamentals. Chronica Horticulturae, 48(4), 11-14.
Navaphanich, U. (2006). Plant tissue culture. (1) Bangkok: Ramkhamhaeng University Press. (in Thai)
Pasek, K. (1999). Sowing carnivorous plant seeds. Retrieved from http://www.bestcarnivorousplants.com
Songsri, O., Jirakiattikul, Y., Rithichai, P., & Itharat, A. (2013). Effect of auxin on In vitro shoot multiplication
and root inductionof Hua-Khao-Yen (Dioscorea birmanica Prain&Burkill). Agricultural Sci. J.,
44(2), 49-52. (in Thai)
Taweerodjanakarn, S., Kongton, K., Thongyai, K., & Samala, S. (2018). In vitro micropropagation of
Drosera burmannii Vahl. Songklanakarin Journal of Plant Science, 5(3), 18-26. (in Thai)
Techapinyawat, S. (2001). Plant Physiology. Bangkok: Publisher Kasetsart University. (in Thai)
Thaweesak, J., Seiichi, S., Hiroyuki, T., & Waraporn, P. (2011). Elicitation effect on production
of plumbagin in in vitro culture of Drosera indica L. Journal of Medicinal Plants
Research, 5(19), 4949-4953.
Tomaz, R., & Marina, D. (2010). Cytokinins and their function in developing seeds. Acta
Chimica Slovenica, 57, 617-629.
Wongchaochant, S. & Phumphothong, S. (2015). In vitro micropropagation of Venus flytrap (Dionaea
muscipula Soland. ex Ellis). In The 53rd Kasetsart University Annual Conference (pp. 590-650). Bangkok: Kasetsart University. (in Thai)
Ziaratmai, S. M., Kunert K. J., & Lall, N. (2009). Elicitation of 7-methyjuglone in Drosera capensis.
South African Journal of Botany, 75, 97-103.
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2019-09-30
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