The Ultrasonic Extraction Method for Determination of Sulfonamide
Abstract
The ultrasonic extraction method for determination of 5 sulfonamides residues in chicken was studied. The amount of sulfonamides was analyzed by high performance liquid chromatographic technique. The extraction parameters such as type of organic solvent, extraction time, volume of organic solvent and extraction temperature were carried out in order to increase the extraction efficiency. In extraction process, the synthetic chicken of 2.0 g was used. The optimum solvent was 5 mL of acetonitrile. The extraction time and extraction temperature were 30 min and 30 °C, respectively. Under the optimum conditions, detection limit and quantification limit were 0.03-0.06 and 0.20-0.40 mg/L, respectively. The linear was in range of 0.20-2.40 mg/L with R2 more than 0.9917. The %RSD and %recovery were 0.41-19.92 and 52.52-151.14%, respectively. The extraction efficiency of this method was more than 90%.References
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3. European Council Regulation 2377/90/EC (1990). A community procedure for the establishment of maximum
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4. Stoev, G., Michailova, A., (2000). Quantitative determination of sulfonamide residues in foods of animal origin by high-performance liquid chromatography with fluorescence detection. Journal of Chromatography A, 871, 37-42.
5. Förster, M., Laabs, V., Lamshöft, M., Pütz, T. and Amelung ,W. (2008). Analysis of aged sulfadiazine residues in soils using microwave extraction and liquid chromatographytandem mass spectrometry. Analytical and Bioanalytical Chemistry 391:1029–1038
6. Raich-Montiu, J., Beltrán, J. L., Prat, M. D, Granados, M. (2010). Studies on the extraction of sulfonamides from agricultural soils. Analytical and Bioanalytical Chemistry. 397, 807-814.
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10. AOAC International. (1993). AOAC peer-verified method program: manual on policies and procedures. United States of America.
