Optimization of Polybutylene Succinate (PBS)-Degrading Enzyme Production from Saccharothrix sp. APL5
Abstract
An actinomycete, Saccharothrix sp. APL5 was isolated from rubbish soil in Thailand and had the ability to degrade polybutylene succinate (PBS) that identified as Saccharothrix texasensis (98% similarity) by 16S rDNA gene analysis. The optimum conditions found for PBS depolymerase production in medium were 1.5% (w/v) PBS as carbon source, 0.12% (w/v) yeast extract as nitrogen source, pH 7 and incubated at 37°C for 96 hours. The highest of specific activity against PBS was determined as 35.6±2.31 U/mg. Strain APL5 could degrade PBS films in basal medium for 56 days at 37°C showed many changes in surface morphology such as erosion and extensive roughening of the surface with pit formation using scanning electron microscopy. The IR spectra of PBS film treated with APL5 showed a broad absorption band in the region of 3400-3000 cm-1(OH stretch) and the less intense peak at of 1740 cm-1(C=O stretch). Partial nucleotides of bioplastic depolymerase gene from strain APL5 was studied. The amino acid of Lpa5 had a highly conserved pentapeptide catalytic triad (Gly-His-Ser-Met-Gly), which has been shown to be part of the esterase-lipase superfamily (serine hydrolase). Keywords : polybutylene succinate, PBS depolymerase, bioplastics, Saccharothrix spReferences
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Staneck, J.L., & Roberts, G.D. (1974). Simplified approach to identification of aerobic actinomycetes by
thin-layer chromatography. Applied Microbiology, 28, 226-231.
Sukrakanchana, L., Sukkhum, S., & Somyoonsap, P. (2011). Isolation of bioplastics-degrading bacteria from compost soil in Thailand. In The 23rd Annual Meeting of the Thai Society for Biotechnology
“Systems Biotechnology: Quality & Success” (TSB 2011). (pp. 252-253). Bangkok, Thailand.
Teeraphatpornchai, T., Nakajima-Kambe, T., Shigeno-Akutsu, Y., Nakayama, M., Nomura, N., Nakahara,
T., & Uchiyama, H. (2003). Isolation and characterization of a bacterium that degrades various
polyester-based biodegradable plastics. Biotechnology Letters, 25, 23-25.
Tokiwa, Y., & Suzuki, T. (1977). Hydrolysis of polyesters by lipases. Nature, 270, 76-78.
Uchida, H., Nakajima-Kambe, T., Shigeno-Akutsu, Y., Nomura, N., Tokiwa, Y., & Nakahara, T. (2000).
Properties of a bacterium which degrades solid poly(tetramethylene succinate)-co-adipate,
a biodegradableplastic. FEMS Microbiology Letters, 189, 25-29.
Williams, D.F. (1981). Enzymic hydrolysis of polylactic acid. Engineering in Medicine, 10, 5-7.
Xu, J., & Guo, B-H. (2010). Microbial succinic acid, its polymer poly(butylene succinate), and applications.
In G-Q. Chen. (Ed.), Plastics from Bacteria: Natural Functions and Applications. (pp.347-388).
Berlin, Heidelberg: Springer-Verlag Berlin Heidelberg
Yan, X., Huang, L-L., Tu, X., Gao, X-N., & Kang, Z-S. (2012). Saccharothrix yanglingensis sp. nov.,
an antagonistic endophytic actinomycete isolated from cucumber plant. Antonie van Leeuwenhoek,
101, 141-146.
Yassin, A.F., Rainey, F.A., Brzezinka, H., Jahnke, K-D, Weissbrodt, H., Budzikiewicz, H., Stackebrandt,
E., & Schaal, K.P. (1995). Lentzea gen. nov., a new genus of the order Actinomyceytales.
International Journal of Systematic Bacteriology, 45, 357-363.
Bradford, M.M. (1976). A rapid method for the quantification of microgram quantities of proteins utilizing the principle of protein-dye binding. Analytical biochemistry, 72, 248-254.
Department of Environmental Quality Promotion. (2018). The Journal of the Network NEVT Conservator.
Retrieved April 30, 2019, from https://www.deqp.go.th/service-portal/environmental-media-system/electronics-detail/?id=100697 (in Thai)
Fujimaki, T. (1998). Processability and properties of aliphatic polyester BIONOLLE synthesized by polycondensation reaction. Polymer Degradation and Stability, 59, 209-214.
Ghosh, S.K., Pal, S., & Ray, S. (2013). Study of microbes having potentiality for biodegradation of plastics. Environmental Science and Pollution Research, 20, 4339-4355.
Hu, X., Thumarat, U., Zhang, X., Tang, M., & Kawai, F. (2010). Diversity of polyester-degrading bacteria in compost and molecular analysis of a thermoactive esterase from Thermobifida alba AHK119.
Applied Microbiology and Biotechnology, 87, 771-779.
Kieser, T., Bibb, M.J., Buttner, M.J., Chater, K.F., & Hopwood, D.A. (2000). Practical Streptomyces Genetics. Norwich: The John Innes Foundation.
Labeda, D.P., Testa, R.T., Lechevalier, M., & Pand, H.A. (1984). Saccharothrix: a new genus of the actinomycetales related to Nocardiopsis. International Journal of Systematic Bacteriology, 34, 426-431.
Lee, C.W., Kimura, Y., & Chung, J-D. (2008). Mechanism of enzymatic degradation of poly(butylene succinate). Macromolecular Research, 16, 651-658.
Lee, S.D., Kim, E.S., Roe, J-H, Kim, J., Kang, S-O, & Hah, Y.C. (2000). Saccharothrix violacea sp. nov., isolated from a gold mine cave, and Saccharothrix albidocapillata comb. nov. International Journal of Systematic and Evolutionary Microbiology, 50, 1315-1323.
Lim, H.A., Raku, T., & Tokiwa,Y. (2005). Hydrolysis of polyesters by serine proteases. Biotechnology Letters, 27, 459-464.
Maeda, H., Yamagata, Y., Abe, K., Hasegawa, F., Machida, M., Ishioka, R., Gomi, K., & Nakajima, T. (2005). Purification and characterization of a biodegradable plastic-degrading enzyme from Aspergillus oryzae. Applied Microbiology and Biotechnology, 67, 778-788.
Mueller, R.J. (2003). Biodegradability of polymers: regulations and methods for testing. In: A. Steinbüchel. (Ed.), Biopolymers. (pp. 365-374). Weinheim: Wiley-VCH.
Nishida, H., & Tokiwa, Y. (1993). Distribution of poly(β-hydroxybutyrate) and poly(ε-caprolactone) aerobic degrading microorganisms in different environments. Journal of Environmental Polymer Degradation, 1, 227-233.
Oda, Y., Naoya, O., Teizi, U., & Kenzo, T. (1997). Polycaprolactone depolymerase produced by the bacterium Alcaligenes faeaclis. FEMS Microbiology Letters, 152, 339-343.
Penkhrue, W., Khanongnuch, C., Masaki, K., Pathom-aree, W., Punyodom, W., & Lumyong, S. (2015). Isolation and screening of biopolymer-degrading microorganisms from northern Thailand. World Journal of Microbiology and Biotechnology, 31, 1431-1442.
Pranamuda, H., Tokiwa, Y., & Tanaka, H. (1995). Microbial degradation of an aliphatic polyester with a high melting point, poly(tetramethylene succinate). Applied and Environmental Microbiology, 61, 1828-1832.
Shirling, E.B., & Gottlieb, D. (1966). Methods for characterization of Streptomyces species.
International Journal of Systematic Bacteriology, 16, 313-340.
Simachaya, W. (2018). Plastic waste management guidelines to promote sustainable use of plastics
in Thailand for the future. Retrieved Jan 15, 2019, from http://www.tei.or.th/tbcsd/event
/180911-tbcsd-VS.pdf (in Thai)
Song D.K., & Sung, Y.K. (1995). Synthesis and characterization of biodegradable poly(1,4-butanediol succinate). Journal of Applied Polymer Science, 56, 1381-1395.
Sriyapai, P., Chansiri, K., & Sriyapai, T. (2018). Isolation and characterization of polyester-based plastics-degrading bacteria from compost soils. Microbiology, 87, 290-300.
Sriyapai, T., Siripoke, S., Chansiri, K., Petchwattana, N., & Sriyapai, P. (2014). Optimization for production of aliphatic polyester-degrading enzyme from Actinomadura sp. strain TF1. Srinakharinwirot Science Journal, 30(2), 103-118.
Staneck, J.L., & Roberts, G.D. (1974). Simplified approach to identification of aerobic actinomycetes by
thin-layer chromatography. Applied Microbiology, 28, 226-231.
Sukrakanchana, L., Sukkhum, S., & Somyoonsap, P. (2011). Isolation of bioplastics-degrading bacteria from compost soil in Thailand. In The 23rd Annual Meeting of the Thai Society for Biotechnology
“Systems Biotechnology: Quality & Success” (TSB 2011). (pp. 252-253). Bangkok, Thailand.
Teeraphatpornchai, T., Nakajima-Kambe, T., Shigeno-Akutsu, Y., Nakayama, M., Nomura, N., Nakahara,
T., & Uchiyama, H. (2003). Isolation and characterization of a bacterium that degrades various
polyester-based biodegradable plastics. Biotechnology Letters, 25, 23-25.
Tokiwa, Y., & Suzuki, T. (1977). Hydrolysis of polyesters by lipases. Nature, 270, 76-78.
Uchida, H., Nakajima-Kambe, T., Shigeno-Akutsu, Y., Nomura, N., Tokiwa, Y., & Nakahara, T. (2000).
Properties of a bacterium which degrades solid poly(tetramethylene succinate)-co-adipate,
a biodegradableplastic. FEMS Microbiology Letters, 189, 25-29.
Williams, D.F. (1981). Enzymic hydrolysis of polylactic acid. Engineering in Medicine, 10, 5-7.
Xu, J., & Guo, B-H. (2010). Microbial succinic acid, its polymer poly(butylene succinate), and applications.
In G-Q. Chen. (Ed.), Plastics from Bacteria: Natural Functions and Applications. (pp.347-388).
Berlin, Heidelberg: Springer-Verlag Berlin Heidelberg
Yan, X., Huang, L-L., Tu, X., Gao, X-N., & Kang, Z-S. (2012). Saccharothrix yanglingensis sp. nov.,
an antagonistic endophytic actinomycete isolated from cucumber plant. Antonie van Leeuwenhoek,
101, 141-146.
Yassin, A.F., Rainey, F.A., Brzezinka, H., Jahnke, K-D, Weissbrodt, H., Budzikiewicz, H., Stackebrandt,
E., & Schaal, K.P. (1995). Lentzea gen. nov., a new genus of the order Actinomyceytales.
International Journal of Systematic Bacteriology, 45, 357-363.
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2019-09-27
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