Isolated Compound and Its Biological Activities from Dendrocnide stimulans
Abstract
Dendrocnide stimulans are belongs in the Family Urticaceae, poisonous tree. There is no data of chemical study and biological activities. This research aims to study the biological activities of extracts of this plants, including inhibition of lung cancer cells (NCI-H187), anti-Herpes simplex virus cytotoxicity, and antimicrobials agents as K. pneumoniae, P. aeruginosa, E. coli, C. albicans and A. baumannii and antioxidant activity and to isolated pure compound from the extract and biological activities of pure compound. The dried and ground leaves and branch samples were collected and extracted with ethanol, respectively. The tested of anti-lung cancer, anti-HSV-1 and cytotoxicity (Vero cell) were used GFP-based method. Five antimicrobial agents, were tested by optical density microplate. The antioxidation test was used DPPH method, measured absorption at wavelength of 517 nm. The isolation of pure compounds was successively separated by using chromatographic techniques. The identification of structure of it was using spectroscopic techniques. The statistics used in the research were mean, percentage, standard deviation, and t-test. According to preliminary studies, it was found that ethanolic leaves extracts showed interesting anti-lung cancer activity at 50 mg / ml with inhibition percentage of 99.49 ± 0.00. In addition, the leaves extract showed moderate cytotoxicity and antioxidant activity. The compound-1 was isolated from ethyl acetate leaves extract. Its structural identification was successively identified by compared with literature of Infrared spectral data and Nuclear Magnetic Resonance Spectral data. Conclusion, Compound-1 was b-sitosterol, showed anti-lung cancer activity of IC50 of more than 50 mg/ml.Keywords : Dendrocnide stimulants, biological activities, b-sitosterolReferences
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Govindarajan, P., & Sarada, D.V.L. (2011). Isolation and characterization of stigmasterol and -sitosterol from Acacia
nilotica (l.) Delile ssp Indica (benth.). Journal of Pharmacy Research, 4(10), 3601-3602.
Hunt, L., Jordan, M., De Jesus, M., & Wurm, F.M. (1999). GFP-expressing mammalian cells for fast, sensitive, noninvasive cell growth assessment in a kinetic mode. Biotechnol and Bioeng, 65, 201-5.
Khamfachuea, K., Trisonthi, P., & Trisonthi, C. (2010. Ethnobotany of the Karen at Ban Chan and Chaem Luang subdistricts, Mae Chaem district, Chiang Mai province. THAI JOURNAL OF BOTANY 2 (Special Issue), 275-297. (inThai)
Phengklai, C. (2018). Toxic plants in Thailand (1). Toxicology center. Botany Department, Office of the Royal Society. Retrieved August 15, 2018, from http://webdb.dmsc.moph.go.th/ifc_toxic/a_tx_1_001c. asp?info_id=86.
Prachuabaree, L. (2008). Medicinal plants of Karang hill tribe in ban Pong-Lueg, Kaeng Krachan District,
Phetchaburi Province. Master of Pharmacy program of Pharmacognosy. Silpakorn University. (inThai)
Saeidnia, S., Manayi, A., Gohari, A. R., & Abdollahi, M. (2014). The Story of Beta-sitosterol- A Review. European Journal of Medicinal Plants, 4(5), 590-609.
Singchai, B., Daengkhwanthong, U., & Pongputthachat, D. (2017). Phytochemicals and Biological Activities of
Opuntia elatior Fruit. Thai Science and Technology Journal, 26(2), 236-245. (inThai)
Tant,i B., Buragohain, K. A., Gurung, L., Kakati, D., Das, A.K., & Borah, S.P. (2010). .Assessment of antimicrobial and antioxidant activities of Dendrocnide sinuate (Blume) Chew leaves - A medicinal plant used by ethnic communities of North East India. Indian Journal of Natural products and Resources., 1(1),17-21.
Wikler, M.A. (2006). Performance Standards for Antimicrobial Susceptibility Testing.
16th ed Informational Supplement. USA: Pennsylvania.
Wikler, M.A., Cockerill, F.R., Craig, W.A., Dudley, M.N., Eliopoulos, G.M., Hecht, D.W., Hindler, J.F., Ferraro, M.J., Swenson, J.M., Low, D.E., Sheehan, D.J., Tenover, F.C., Turnidge, J.D., Weinstein, M.P.& Zimmer, B.L. (2006). Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically; Approved Standard. 7th Edition. USA: Pennsylvania.
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2020-01-08
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