Screening of Xylose-Utilizing Oleaginous Yeasts from Fermented Bio-extracts for Microbial Oil Production
Abstract
Biodiesel is a renewable alternative to petroleum-based diesel fuel and environment friendly energy which made from various vegetable oils, animal fats and waste cooking oils through transesterification. Nowadays, microbial oils from oleaginous microorganisms are recognized as promising alternative biodiesel feedstock, especially, oleaginous yeast oils. In this study, oleaginous xylose-utilizing yeasts which produce microbial oil were screened from fermented bio-extract samples. Nineteen differently morphological yeast isolates were isolated on YPD agar medium and twelve yeast isolates could grow and utilize xylose as carbon sources on xylose medium. Microbial oil production of xylose-utilizing yeasts was investigated on nitrogen-limiting medium containing 30.0 g/L of xylose as a sole carbon source; initial pH 5.5; temperature at 30◦C and orbital shaking at 150 rpm for 120 h. It was found that the yeast isolate FBY3-3 accumulated the intracellular lipid about 23.21% by dry cell weight that called oleaginous yeast. The isolate FBY3-3 was identified as a Pichia kudriavzevii FBY3-3. The extracted oils of P. kudriavzevii FBY3-3 predominantly contained long chain fatty acids such as palmitic acid (C16:0) and oleic acid (C18:1) that comparable to conventional vegetable oils. The results suggest that P. kudriavzevii FBY3-3 has a potential for microbial oil production and could be used as alternative biodiesel feedstock. Keywords : oleaginous yeasts, xylose, microbial oils, screeningReferences
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Journal of Cleaner Production, 133, 899-909.
Beopoulos A., Cescut, J., Haddouche, R., Uribelarrea, J. L. Moilna-Jouve, C. and Nicaud, J. M. (2009).Yarrowia lipolytica as a model for bio-oil production.Progress in Lipid Research, 48(6), 375-387.
Chan, G. F., Gan, H. M., Ling, H. L. and Rashid, N. A. (2012) Genome sequence of Pichia kudriavzeviiM12,
a potential producer of bioethanol and phytase. Eukaryotic Cell, 11(10), 1300-1301.
Chang, Y. H., Chang, K. S., Hsu, C. L., Chuang, L. T., Chen, C. Y., Huang, F. U. and Jang, H. D. (2013). A comparative study on batch and fed-batch cultures of oleaginous yeast Cryptococcus sp. in glucose-based media and corncob hydrolysate for microbial oil production.Fuel, 105(1), 711-717.
Chanklan, R., Kungkaew, P., Am-In, S. and Jindamorakot, Sasitorn.(2012) Diversity of yeasts in the nature education center for mangrove conservation and ecotourism, Chonburi Province.Thai Journal of Science and Technology, 1(3), 155-168.
Dangpram, P., Chantorn S. and Nitisinprasert, S. (2013). Effects of Types and Content of Agricultural Wastes for Oligosaccharide Production by Crude Enzyme from Penicillium oxalicum KUB-SN2-1. KMUTT Research and Development Journal, 36(1), 73-84.
Davies, R. and Holdsworth, J. E. (1992). Synthesis of lipids in yeasts: Biochemistry, physiology and production. Advances in Applied Lipid Research, 1, 119-159.
Gao, D., Zeng, J., Zheng, Y., Yu, X. and Chen, S. (2013). Microbial lipid production from xylose by Mortierella isabellina. Bioresource Technology, 133, 315-321.
Hahn-Hägergal, B., Karhumaa, K., Fonseca, C., Spencer-Martins, I. and Gorwa-Grauslund, M. F. (2007). Towards industrial pentose-fermenting yeast strains. Applied Microbiology and Biotechnology, 74(5), 937-953.
Kitcha, S. and Cheirsilp, B. (2011).Screening of oleaginous yeasts and optimization for lipid production using crude glycerol as a carbon source.Energy Procedia, 9, 274-282.
Kraisintu, P., Yongmanitchai, W. and Limtong, S. (2010).Selection and optimization for lipid production of anewly isolated oleaginous yeast, Rhodosposidium toruloides DMKU3-TK16. Kasetsart Journal (Natural Science), 44, 436-445.
KU-Biodiesel Project. (2008). Biodiesel. Retrieved February 5, 2019, from http:// http://www.biodiesel.rdi.ku.ac.th/.
Kumar, D., Singh, B. and Korstad, J. (2017).Utilization of lignocellulosic biomass by oleaginous yeast and bacteria for production of biodiesel and renewable diesel.Renewable and Sustainable Energy Reviews, 73,
654-671.
Kurtzman, C. P. and Fell, J. W. (2000).The yeasts.A Taxonomic study.4th revised and enlarged edition. Amsterdum, Lausanne, New York, Oxford, Shannon, Singapore, Tokyo: Elsevier.
Kurtzman, C. P., Fell, J. W. and Boekhout, T.(2011). The Yeasts: a Taxonomic Study. 5th edition.San Diego: Elsevier.
Leesing, R. and Nantaso, N. (2011).Isolation and cultivation of oleaginous yeast for microbial oil production.KKU Research Journal, 16(2), 112-126.
Li, M., Liu, G. L., Chi, Z. and Chi, Z. M. (2010).Single cell oil production from hydrolysate of cassava starch by marine-derived yeast Rhodotorula mucilaginosa TYJ15a.Biomass and Bioenergy, 34(1), 101-107.
Ma, F. and Hanna, M. A. (1999). Biodiesel production: A review. Bioresource Technology, 70(1), 1-15.
Miller, G. L. (1959). Use of dinitrosalicylic acid reagent for determination of reducing sugar.Analytical Chemistry, 31(3), 426-428.
Pan, L. X., Yang, D. F., Shao, L., Li., W., Chen, G. G. and Liang, Z. Q. (2009).Isolation of the oleaginous yeasts from the soil and studies of their lipid-producing capacities.Food Technology and Biotechnology, 47(2), 215-220.
Papanikolaou, P. and Aggelis, G. (2011). Lipids of oleaginous yeasts. Part I: Biochemistry of single cell oil production. 113(8), European Journal of Lipid Science and Technology, 113(8), 1031 – 1051.
Polprasert, S. (2014). Pretreatment of lignocellulosic materials for ethanol production. Thai Science and Technology Journal, 22(5), 641-649.
Poontawee, R., Yongmanitchai, W. and Limtong, S. (2016). Efficient oleaginous yeasts for lipid production lignocellulosic sugars and effects of lignocelluloses degradation compounds on growth and lipid production. Process Biochemistry, 53, 44-60.
Qin, L., Lui, L. Zeng, A-P.and Wei, D. (2017). From low-cost substrate to single cell oils synthesized by oleaginous yeasts. Bioresources Technology, 245, 1507-1519.
Sankh, S., Thiru, M., Saran, S. and Rangaswamy, V. (2013). Biodiesel production from a newly isolated Pichia
kudriavzevii strain. Fuel, 106, 690-696.
Saran, S., Mathur, A., Dalal, J. and Saxena, R. K. (2017). Process optimization for cultivation and oil accumulation in an oleaginous yeast Rhodosporidium toruloides A29. Fuel, 188, 324-331.
Sriariyanun, M., Phetsom, J. and Kongruang, S. (2014). “Lipid production for second generation biodiesel by the oleaginous yeast.Journal of Science and Technology Mahasarakham University,33(3), 300-306.
Sriwongchai, S. (2015). Microbial oils as a new biodiesel feedstock: Alternative for renewable energy. The Science Journal of Phetchaburi Rajabhat University, 12(1), 72-83.
Sriwongchai, S., Pokethitiyook, P., Kruatrachue, M., Bajwa, P. K. and Lee, H. (2013).Screening of selected oleaginous yeasts for lipid production from glycerol and some factors which affect lipid production by Yarrowia lipolytica strains.Journalof Microbiology, Biotechnology and Food Sciences, 2(5), 2344-2348.
Sriwongchai S., Pokethitiyook, P., Pugkaew, W., Kruatrachue, M. and Lee, H. (2012).Optimization of lipid production in the oleaginous bacterium Rhodococcus erythropolis growing on glycerol as the sole carbon source. African Journal of Biotechnology, 11(79), 14440-14447.
Sriwongchai, S., Prasongsuk, S., Leerat, N. and Kitleartpornpairoat, R. (2018). Possibility of soil mangrove-derived oleaginous yeast Rhodotorula mucilaginosa on nutritionally optimized medium for lipid production for alternative biodiesel feedstock. Burapha Science Journal, 23(1), 304-317.
Steen, E. J., Kang, Y., Bokinsky, G., Hu, Z., Schirmer, A., McClure, A., Del Cardayre, S. B. and Keasling, J. D. (2010). Microbial Production of fatty-acid derived fuels and chemicals from plant biomass.Nature, 463, 559-562
Suriyapan, P., Virunanon, C., Chulalaksananukul, S and Chulalaksananukul, W. (2011).Screening of thermotolerant xylose-utilizing yeasts for ethanol production.In Proceeding of 49th Kasetsart University Annual Conference: Science. (pp 400-406). Thailand: Bangkok.
Tancho, A. (2013). Applied Natural Agriculture Textbook: Principles of Practical Techniques in Thailand. (3rd edition). Chiang Mai: Northern Natural Agriculture Network.
Taskin, M., Ortucu, S., Aydogan, M. N. and Arslan, N. P. (2016).Lipid from sugar beet molasses under non-aseptic culture conditions using the oleaginous yeast Rhodotorula glutinis TR29.Renewable Energy, 99, 198-204.
Wang, C. L., Li, Y., Xin, F. H., Liu, Y. Y. and Chi, Z. M. (2014). Evaluation of single cell oil from Aureobasidium pullulans var. melanogenum P10 isolated from mangrove ecosystems for biodiesel production. Process Biochemistry, 49(5), 725-731.
Wang, G-Y., Chi, Z., Song, B., Wang, Z-P., Chi, Z-M. (2012). High level lipid production by a novel inulinase-producing yeast Pichia guilliermondii Pcla22. Bioresource Technology, 124, 77-82.
Yamada, R., Yammauchi, A., Kashihara, T. and Ogino, H. (2017). Evaluation of lipid production from xylose and glucose/xylose mixed sugar in various oleaginous yeasts and improvement of lipid production by UV mutagenesis. Biochemical Engineering Journal, 128, 76-82.
Journal of Cleaner Production, 133, 899-909.
Beopoulos A., Cescut, J., Haddouche, R., Uribelarrea, J. L. Moilna-Jouve, C. and Nicaud, J. M. (2009).Yarrowia lipolytica as a model for bio-oil production.Progress in Lipid Research, 48(6), 375-387.
Chan, G. F., Gan, H. M., Ling, H. L. and Rashid, N. A. (2012) Genome sequence of Pichia kudriavzeviiM12,
a potential producer of bioethanol and phytase. Eukaryotic Cell, 11(10), 1300-1301.
Chang, Y. H., Chang, K. S., Hsu, C. L., Chuang, L. T., Chen, C. Y., Huang, F. U. and Jang, H. D. (2013). A comparative study on batch and fed-batch cultures of oleaginous yeast Cryptococcus sp. in glucose-based media and corncob hydrolysate for microbial oil production.Fuel, 105(1), 711-717.
Chanklan, R., Kungkaew, P., Am-In, S. and Jindamorakot, Sasitorn.(2012) Diversity of yeasts in the nature education center for mangrove conservation and ecotourism, Chonburi Province.Thai Journal of Science and Technology, 1(3), 155-168.
Dangpram, P., Chantorn S. and Nitisinprasert, S. (2013). Effects of Types and Content of Agricultural Wastes for Oligosaccharide Production by Crude Enzyme from Penicillium oxalicum KUB-SN2-1. KMUTT Research and Development Journal, 36(1), 73-84.
Davies, R. and Holdsworth, J. E. (1992). Synthesis of lipids in yeasts: Biochemistry, physiology and production. Advances in Applied Lipid Research, 1, 119-159.
Gao, D., Zeng, J., Zheng, Y., Yu, X. and Chen, S. (2013). Microbial lipid production from xylose by Mortierella isabellina. Bioresource Technology, 133, 315-321.
Hahn-Hägergal, B., Karhumaa, K., Fonseca, C., Spencer-Martins, I. and Gorwa-Grauslund, M. F. (2007). Towards industrial pentose-fermenting yeast strains. Applied Microbiology and Biotechnology, 74(5), 937-953.
Kitcha, S. and Cheirsilp, B. (2011).Screening of oleaginous yeasts and optimization for lipid production using crude glycerol as a carbon source.Energy Procedia, 9, 274-282.
Kraisintu, P., Yongmanitchai, W. and Limtong, S. (2010).Selection and optimization for lipid production of anewly isolated oleaginous yeast, Rhodosposidium toruloides DMKU3-TK16. Kasetsart Journal (Natural Science), 44, 436-445.
KU-Biodiesel Project. (2008). Biodiesel. Retrieved February 5, 2019, from http:// http://www.biodiesel.rdi.ku.ac.th/.
Kumar, D., Singh, B. and Korstad, J. (2017).Utilization of lignocellulosic biomass by oleaginous yeast and bacteria for production of biodiesel and renewable diesel.Renewable and Sustainable Energy Reviews, 73,
654-671.
Kurtzman, C. P. and Fell, J. W. (2000).The yeasts.A Taxonomic study.4th revised and enlarged edition. Amsterdum, Lausanne, New York, Oxford, Shannon, Singapore, Tokyo: Elsevier.
Kurtzman, C. P., Fell, J. W. and Boekhout, T.(2011). The Yeasts: a Taxonomic Study. 5th edition.San Diego: Elsevier.
Leesing, R. and Nantaso, N. (2011).Isolation and cultivation of oleaginous yeast for microbial oil production.KKU Research Journal, 16(2), 112-126.
Li, M., Liu, G. L., Chi, Z. and Chi, Z. M. (2010).Single cell oil production from hydrolysate of cassava starch by marine-derived yeast Rhodotorula mucilaginosa TYJ15a.Biomass and Bioenergy, 34(1), 101-107.
Ma, F. and Hanna, M. A. (1999). Biodiesel production: A review. Bioresource Technology, 70(1), 1-15.
Miller, G. L. (1959). Use of dinitrosalicylic acid reagent for determination of reducing sugar.Analytical Chemistry, 31(3), 426-428.
Pan, L. X., Yang, D. F., Shao, L., Li., W., Chen, G. G. and Liang, Z. Q. (2009).Isolation of the oleaginous yeasts from the soil and studies of their lipid-producing capacities.Food Technology and Biotechnology, 47(2), 215-220.
Papanikolaou, P. and Aggelis, G. (2011). Lipids of oleaginous yeasts. Part I: Biochemistry of single cell oil production. 113(8), European Journal of Lipid Science and Technology, 113(8), 1031 – 1051.
Polprasert, S. (2014). Pretreatment of lignocellulosic materials for ethanol production. Thai Science and Technology Journal, 22(5), 641-649.
Poontawee, R., Yongmanitchai, W. and Limtong, S. (2016). Efficient oleaginous yeasts for lipid production lignocellulosic sugars and effects of lignocelluloses degradation compounds on growth and lipid production. Process Biochemistry, 53, 44-60.
Qin, L., Lui, L. Zeng, A-P.and Wei, D. (2017). From low-cost substrate to single cell oils synthesized by oleaginous yeasts. Bioresources Technology, 245, 1507-1519.
Sankh, S., Thiru, M., Saran, S. and Rangaswamy, V. (2013). Biodiesel production from a newly isolated Pichia
kudriavzevii strain. Fuel, 106, 690-696.
Saran, S., Mathur, A., Dalal, J. and Saxena, R. K. (2017). Process optimization for cultivation and oil accumulation in an oleaginous yeast Rhodosporidium toruloides A29. Fuel, 188, 324-331.
Sriariyanun, M., Phetsom, J. and Kongruang, S. (2014). “Lipid production for second generation biodiesel by the oleaginous yeast.Journal of Science and Technology Mahasarakham University,33(3), 300-306.
Sriwongchai, S. (2015). Microbial oils as a new biodiesel feedstock: Alternative for renewable energy. The Science Journal of Phetchaburi Rajabhat University, 12(1), 72-83.
Sriwongchai, S., Pokethitiyook, P., Kruatrachue, M., Bajwa, P. K. and Lee, H. (2013).Screening of selected oleaginous yeasts for lipid production from glycerol and some factors which affect lipid production by Yarrowia lipolytica strains.Journalof Microbiology, Biotechnology and Food Sciences, 2(5), 2344-2348.
Sriwongchai S., Pokethitiyook, P., Pugkaew, W., Kruatrachue, M. and Lee, H. (2012).Optimization of lipid production in the oleaginous bacterium Rhodococcus erythropolis growing on glycerol as the sole carbon source. African Journal of Biotechnology, 11(79), 14440-14447.
Sriwongchai, S., Prasongsuk, S., Leerat, N. and Kitleartpornpairoat, R. (2018). Possibility of soil mangrove-derived oleaginous yeast Rhodotorula mucilaginosa on nutritionally optimized medium for lipid production for alternative biodiesel feedstock. Burapha Science Journal, 23(1), 304-317.
Steen, E. J., Kang, Y., Bokinsky, G., Hu, Z., Schirmer, A., McClure, A., Del Cardayre, S. B. and Keasling, J. D. (2010). Microbial Production of fatty-acid derived fuels and chemicals from plant biomass.Nature, 463, 559-562
Suriyapan, P., Virunanon, C., Chulalaksananukul, S and Chulalaksananukul, W. (2011).Screening of thermotolerant xylose-utilizing yeasts for ethanol production.In Proceeding of 49th Kasetsart University Annual Conference: Science. (pp 400-406). Thailand: Bangkok.
Tancho, A. (2013). Applied Natural Agriculture Textbook: Principles of Practical Techniques in Thailand. (3rd edition). Chiang Mai: Northern Natural Agriculture Network.
Taskin, M., Ortucu, S., Aydogan, M. N. and Arslan, N. P. (2016).Lipid from sugar beet molasses under non-aseptic culture conditions using the oleaginous yeast Rhodotorula glutinis TR29.Renewable Energy, 99, 198-204.
Wang, C. L., Li, Y., Xin, F. H., Liu, Y. Y. and Chi, Z. M. (2014). Evaluation of single cell oil from Aureobasidium pullulans var. melanogenum P10 isolated from mangrove ecosystems for biodiesel production. Process Biochemistry, 49(5), 725-731.
Wang, G-Y., Chi, Z., Song, B., Wang, Z-P., Chi, Z-M. (2012). High level lipid production by a novel inulinase-producing yeast Pichia guilliermondii Pcla22. Bioresource Technology, 124, 77-82.
Yamada, R., Yammauchi, A., Kashihara, T. and Ogino, H. (2017). Evaluation of lipid production from xylose and glucose/xylose mixed sugar in various oleaginous yeasts and improvement of lipid production by UV mutagenesis. Biochemical Engineering Journal, 128, 76-82.
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2019-02-26
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