Total Phenolic Content Antioxidant and Antimicrobial Activities from the Madhuca esculenta Leaves Extracts
Abstract
The total phenolic content, antioxidant and antimicrobial activities from the Madhuca esculenta leaves extracted by methanol and hexane were studied. The results indicated that the methanol extracted had the highest antioxidant activity were 91.356±0.268% with total phenolic contents 11.332±0.327 mgGAE/100 mg sample. Then partial purification were performed by Quick column chromatography with solvent 100% Hexane to 70% MeOH:EtOAc were eluted (FR-1 to FR-3). The DPPH assay showed that FR-3 gave the highest antioxidant activity were 83.488±0.154% with total phenolic contents 4.952±0.299 mgGAE/100 mg sample. The antimicrobial activities (Bacillus cereus, Staphylococcus aureus, Escherichia coli, Bacillus subtilis and Salmonella typhimurium) from the Madhuca esculenta leaves extracted found that the FR-1 showed the highest antipathogenic bacterial activity against bacteria B. cereus and B. subtilis. Keywords : Madhuca esculenta, antioxidant, antimicrobial, quick column chromatographyReferences
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Chareanphon, P and Wongkrachang, K. (2015). The Investigation of the Extraction Solvent System of Total Phenolics and Flavonoids–Rich Extracts and Antioxidant Activity from Tagetes erecta leaves. Chemistry Department, Pibulsongkram Rajabhat University. (in Thai)
Jung, B. K., Jong, B. K., Kang, J. C., Gabriele, M. K. and Anthony, D. W. (2006). Antioxidant Activity of 3,4,5-Trihydroxy benzaldehyde Isolated from Geum japonicum. Journal of food & Drung Anal ,14(2), 190-193.
Kong, K. W., Khoo, H. E., Prasad, N. K., Chew, L. Y. and Amin, l. (2013). Total phenolics and Antioxidant Activities of pouteria campechiana fruit parts. Sains Malaysiana, 42(2), 123–127.
Kongsai, S. and Chalard. C. (2013). Antibacterial Activities of the Crude Extract ofAcrostichumaureum L.Growing at RajamangalaUniversity of Technology Srivijaya, Trang Campus. RMUTP Research Journal Special Issue. 363-368. (in Thai)
Lopes, G. K. B., Schulman, H. M. and Lima, M. H. (1999). Polyphenol tannic acid inhibition hydroxyl radical formation from Fenton reaction by complexing ferrous ions. Bio chem. Biophys Acta, 1426, 475-482.
Luo, X. D., Margaret, J. B. and Edward, J. K. (2002). Polyphenol Antioxidants from the fruits of Chrysophylum cainito L. (star apple). Journal of Agricultural and food chemistry, 13;50(6),1379-82.
Mrinal, S., Mahesh, H., Kishore, K. C., Jinsha, K., Souvari, B., Bibha, C. and Sathees, C. R. (2014). Sapodilla Plum (Achras sapota) Induces Apoptosis in Cancer Cell Lines and Inhibits Tumor Progression in Mice. Scientific Reports, 6147(4). 1-7.
Pramkraisorn, P. and Wasupongphan, W. (2015). Antioxidant from 14 kinds of Thai herbs. Chemistry Department, Faculty of Science. Saim University. APHEIT Conference.356-364 (in Thai)
Royal Botanic Garden (Kew).2018.Herbalium catalogue [Online] Available http://specimens.kew.org/herbarium/K00077 (9 October 2018)
Sittikijyothin, W. and Cherdwongcharoensuk, D. (2011). Free Radical Scavenging Activity of Seed Coat Extracts of Sweet and Sour Tamarinds . Burapha Science Journal, 16(1), 47–55. (in Thai)
Suanlaksana. Retrieved 20June 2016, from http://www.bloggang.com/viewdiary.php?id=suanlaksana&month=02-2011&date=12&group=7&gblog=1
Whangumnuayporn, T. and Chaiprasong, R. (2007). Antimicrobial and Antioxidant Activities from Thai herbs. Faculty of Science The University of the Thai Chamber of Commerce. (in Thai)
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2018-10-25
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บทความวิจัยจากการประชุมวิชาการระดับชาติ"วิทยาศาสตร์วิจัย"ครั้งที่ 10
