Effects of Cytokinnin and Sucrose on Tissue Culture of Rosa chinensis Jacq.var. minima Voss.
Abstract
The effects of cytokinin & sucrose on tissue culture of fairy rose (Rosa chinensis Jacq.var. minima Voss.) was conducted in sterile conditions. Nodal segments were cultured on Murashige & Skoog (MS) medium supplemented with different concentrations (0, 0.5, 1.0, 1.5, 2.0 mg/L) of 6-Benzyladenine (BA), Thidiazuron (TDZ) & Kinetin (KN) at 25±2 ºC & 16 hours of light per day with light Intensity at 1,200 lux for 4 weeks. From the result, it was found that all experiment could induce shoot 100%. Specifically, MS supplemented with TDZ at a concentration of 1.5 mg/L had the highest average number of shoots was 4.50±1.93 shoots/explant & significantly different from the other treatments. While MS medium supplemented with 1.5 mg/L TDZ could induce the highest average shoot length of 5.89±2.34 mm & statistically significant differences with other groups. When nodal segment were cultured on MS medium supplemented with 1.5 mg/L TDZ & sucrose concentrations at 30, 40, 50 & 60 g/L for 6 weeks. It was shown that, nodal segments were cultured on MS supplemented with TDZ at 1.5 mg/L & sucrose concentration at 40 g/L had the highest average shoot number of 2.73-2.77 shoot/explant. However, it can be seen that MS medium supplemented with sucrose at all concentrations could induce the average shoot length without statistically significant difference & no flowering occurred. Keywords : Rosa chinensis Jacq.var. minima Voss., cytokinin, sucroseReferences
Abu-Romman, S.M., Al-Hadid, K.A., & Arabiyyat, .A.R. (2015). Kinetin is the Most Effective Cytokinin on Shoot Multiplication from Cucumber. Journal of Agricultural Science, 7.
Al-Khalifah, N.S., Hadi, S. & Khan, F. (2005). Influence of Sucrose Concentration on in vitro Growth of Five Rose (Rosa hybrida L.) .Cultivars. Plant Tissue Cult. ,15(1), 43-49.
Barna K. S. & Wakhlu A. K. (1995). Effects of thidiazuron on micropropagation of rose. In Vitro Celt., 3,
44-46.
Boonnak, S. (2013). Plant tissue culture & plant gene transfer . Khon Kaen: Khon Kaen University. (in Thai)
Buddharak, P.(2013). Plant culture tissue. Chiang mai : Nopburikarpimp. (in Thai)
Chatsuwan, Y. & Rungrachakanont, K. (2014). Micropropagation & In Vitro flowering of Miniature Rose. In The 8th Ubon Ratchatnani University research conference. (pp. 33-40). Ubon Ratchatnani : Ubon Ratchatnani University. (in Thai)
Hesar, A.A., B Kaviani, B., Tarang, A. & Zanjan, S.B.. (2011). Effect of different concentrations of kinetin on regeneration of ten weeks (Matthiola incana). Plant Omics Journal, 4, 236-238.
Jabbarzadeh, Z. & Khosh-Khui, M. (2005). Factors affecting tissue culture of Damask rose (Rosadamascena Mill.). Scientia Horticulturae. 105, 475–482.
Jafar Jaskani, M., Qasim, M., Sherani, J., Hussain, Z & Abbas, H. (2005). Effact of growth hormones on shoot proliferation of rose cultivars. Pak. J. Bot., 37(4), 875-881.
Kanchanapoom, K., Sakpeth, P. & Kanchanapoom, K. (2010). In vitro flowering of shoots regenerated from cultured nodal explants of Rosa hybrida cv. ‘Heirloom’ . Science Asia, 36, 161–164.
Kharde, A.V & Kshirsagar, A.B.. (2014). Effect of BAP & kinetin on nodal culture of Rosa hybrida L.
Bionano Frontier, 2, 254-257.
Kongpraphrut, S. (2008). Factors Influencing the Growth of Propagated Shoots & In Vitro Flowering of My Valentine Rose. Thesis of Master of Science, Plant science, Prince of Songkla university. (in Thai)
Manole, C., Bălan, V., Tudora, C., Buţu, M., Fidler, G., Rodino, S., Golea, D. & Buţu, A. (2011). Influence of sucrose concentration on in vitro multiplication of Ribers rubrum species, 2(4) ,73-74 .
Ozel, C.A. & Arsian, O. (2006). Efficient micropropagation of english shrub rose “Heritage” under in vitro conditions. International Journal of Agriculture & Biology, 8(5), 626-629.
Pongthi, P. & Pongsawat, S. (2010). Culture tissue In Vitro flowering of Miniature Rose. In The 3rd Rajamangala University Of Technology national conference. Bangkok : Rajamangala university of technology. (in Thai)
Sivanesan, I., Park, S.W.. (2015). Optimizing factors affecting adventitious shoot regeneration, in vitro flowering & fruiting of Withania somnifera (L.) Dunal. Industrial Crops & Products, 76, 323–328
Sumaryono, Muslihatin, W. & Ratadewi, D. (2012). Effect of Carbohydrate Source on Growth & Performance of In Vitro Sago Palm (Metroxylon sagu Rottb.) Plantlets. HAYATI Journal of Biosciences, 19(2), 88-92.
Tolera, B., Diro, M.,& Belew, D.( 2014). Effects of 6-Benzyl aminopurine & Kinetin on In Vitro Shoot Multiplication of Sugarcane (Saccharum officinarum L.) Varieties. Adv Crop Sci Tech, 2.
Vu, N.H., Anh, P.H. & Nhut, D.T. (2006). The role of sucrose & different cytokinins in the in vitro floral morphogenesis of rose (hybrid tea) cv. ‘First Prize’. Plant Cell, Tissue & Organ Culture, 87 (3),
315-320.
Wang, G. Y., Yuan, M. F. & Hong, Y. (2001). In vitro flower induction in roses. In vitro cell, 38, 513 -518
Watanaphoot, N.(2002). The flowering plant handbook. Bangkok: O. S. Printing House. (in Thai)
Yadollahi, A., Omidi , M. & Eftekhari, M. (2015). Effect of nutrient medium & concentrations of plant growth regulators on micropropagation of Rosa damascena Mill. cv. ‘Kashan’. Retrieved 20 April 2018, from https://www.researchgate.net/publication/279868651.
Yensai, P. & Suraninpong, P. (2014).Effect of Benzyladenine & Thidiazuron on Multiple Shoot Induction in “Kluai Chang” In Vitro. Khon Kaen Agriculture Journal, 3 ,157-161.(in Thai)
Yooktanont, S. (1995). Rose,queen of flower plant . Bangkok: baanlaesuan.(in Thai)
Zeng, S.J., Liang, S., Zhang, Y.Y., Wu, K.L., Teixeira, Da Silva J.A., & Duan, J. (2013). In vitro flowering red miniature rose. Biologia Plantarum, 57(3), 401-40
Al-Khalifah, N.S., Hadi, S. & Khan, F. (2005). Influence of Sucrose Concentration on in vitro Growth of Five Rose (Rosa hybrida L.) .Cultivars. Plant Tissue Cult. ,15(1), 43-49.
Barna K. S. & Wakhlu A. K. (1995). Effects of thidiazuron on micropropagation of rose. In Vitro Celt., 3,
44-46.
Boonnak, S. (2013). Plant tissue culture & plant gene transfer . Khon Kaen: Khon Kaen University. (in Thai)
Buddharak, P.(2013). Plant culture tissue. Chiang mai : Nopburikarpimp. (in Thai)
Chatsuwan, Y. & Rungrachakanont, K. (2014). Micropropagation & In Vitro flowering of Miniature Rose. In The 8th Ubon Ratchatnani University research conference. (pp. 33-40). Ubon Ratchatnani : Ubon Ratchatnani University. (in Thai)
Hesar, A.A., B Kaviani, B., Tarang, A. & Zanjan, S.B.. (2011). Effect of different concentrations of kinetin on regeneration of ten weeks (Matthiola incana). Plant Omics Journal, 4, 236-238.
Jabbarzadeh, Z. & Khosh-Khui, M. (2005). Factors affecting tissue culture of Damask rose (Rosadamascena Mill.). Scientia Horticulturae. 105, 475–482.
Jafar Jaskani, M., Qasim, M., Sherani, J., Hussain, Z & Abbas, H. (2005). Effact of growth hormones on shoot proliferation of rose cultivars. Pak. J. Bot., 37(4), 875-881.
Kanchanapoom, K., Sakpeth, P. & Kanchanapoom, K. (2010). In vitro flowering of shoots regenerated from cultured nodal explants of Rosa hybrida cv. ‘Heirloom’ . Science Asia, 36, 161–164.
Kharde, A.V & Kshirsagar, A.B.. (2014). Effect of BAP & kinetin on nodal culture of Rosa hybrida L.
Bionano Frontier, 2, 254-257.
Kongpraphrut, S. (2008). Factors Influencing the Growth of Propagated Shoots & In Vitro Flowering of My Valentine Rose. Thesis of Master of Science, Plant science, Prince of Songkla university. (in Thai)
Manole, C., Bălan, V., Tudora, C., Buţu, M., Fidler, G., Rodino, S., Golea, D. & Buţu, A. (2011). Influence of sucrose concentration on in vitro multiplication of Ribers rubrum species, 2(4) ,73-74 .
Ozel, C.A. & Arsian, O. (2006). Efficient micropropagation of english shrub rose “Heritage” under in vitro conditions. International Journal of Agriculture & Biology, 8(5), 626-629.
Pongthi, P. & Pongsawat, S. (2010). Culture tissue In Vitro flowering of Miniature Rose. In The 3rd Rajamangala University Of Technology national conference. Bangkok : Rajamangala university of technology. (in Thai)
Sivanesan, I., Park, S.W.. (2015). Optimizing factors affecting adventitious shoot regeneration, in vitro flowering & fruiting of Withania somnifera (L.) Dunal. Industrial Crops & Products, 76, 323–328
Sumaryono, Muslihatin, W. & Ratadewi, D. (2012). Effect of Carbohydrate Source on Growth & Performance of In Vitro Sago Palm (Metroxylon sagu Rottb.) Plantlets. HAYATI Journal of Biosciences, 19(2), 88-92.
Tolera, B., Diro, M.,& Belew, D.( 2014). Effects of 6-Benzyl aminopurine & Kinetin on In Vitro Shoot Multiplication of Sugarcane (Saccharum officinarum L.) Varieties. Adv Crop Sci Tech, 2.
Vu, N.H., Anh, P.H. & Nhut, D.T. (2006). The role of sucrose & different cytokinins in the in vitro floral morphogenesis of rose (hybrid tea) cv. ‘First Prize’. Plant Cell, Tissue & Organ Culture, 87 (3),
315-320.
Wang, G. Y., Yuan, M. F. & Hong, Y. (2001). In vitro flower induction in roses. In vitro cell, 38, 513 -518
Watanaphoot, N.(2002). The flowering plant handbook. Bangkok: O. S. Printing House. (in Thai)
Yadollahi, A., Omidi , M. & Eftekhari, M. (2015). Effect of nutrient medium & concentrations of plant growth regulators on micropropagation of Rosa damascena Mill. cv. ‘Kashan’. Retrieved 20 April 2018, from https://www.researchgate.net/publication/279868651.
Yensai, P. & Suraninpong, P. (2014).Effect of Benzyladenine & Thidiazuron on Multiple Shoot Induction in “Kluai Chang” In Vitro. Khon Kaen Agriculture Journal, 3 ,157-161.(in Thai)
Yooktanont, S. (1995). Rose,queen of flower plant . Bangkok: baanlaesuan.(in Thai)
Zeng, S.J., Liang, S., Zhang, Y.Y., Wu, K.L., Teixeira, Da Silva J.A., & Duan, J. (2013). In vitro flowering red miniature rose. Biologia Plantarum, 57(3), 401-40
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2018-06-08
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