Artificial Seed Production from Nodal Explant of Stevia rebaudiana Bertoni
Abstract
This study describes the investigation on the artificial seed production from nodal explants of Stevia rebaudiana Bertoni with focus on effects of sucrose concentrations and storage temperatures on germination and growth and development of shoot. Nodal explants were encapsulation by immersion in liquid MS medium supplemented with different concentration of sucrose and 2% sodium alginate. The artificial seeds were kept in refrigerator at 10°C for 7 days before cultured on agar MS medium at 25°C and 16 hours of light per day for 4 weeks. It was found that 1% sucrose was the optimum concentration for germination and growth and development of shoot. Then, artificial seeds (with 1% sucrose) were stored at different temperatures for 7 days before cultured on agar MS medium for 4 weeks. Artificial seeds stored at 0°C showed the highest of germination rate 80% Keywords : synthesis seed, alginate, preservationReferences
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Chotikadachanarong K. and Dheeranupattana S. (2013). Micropropagation and acclimatization of Stevia rebaudiana Bertoni. Pakistan Journal of Biological Sciences, 16(7), 887-890.
Hudi, A. K. M. N., Rahman, M. and Bari, M. A. (2007). Effect of carbon source in alginate bead on synthetic seed germination in eggplant (Solanum melongena L.). Journal of Plant Sciences, 2(5), 538-544.
Manoj, K. R., Jaiswal, V.S. and Jaiswal, U. (2008). Effect of ABA and sucrose on germination of encapsulated somatic embryos of guava (Psidium guajava L.) Scientia Horticulturae, 117, 302–305.
Maqsood, M., Mujib, A. and Khusrau, M. (2015). Preparation and low temperature short-term storage for synthetic seeds of Caladium bicolor. Not Sci Biol, 7(1), 90-95.
Miyagawa, H., Fujioka, N., Kohda, H., Yamasaki, K., Taniguchi, K. and Tanaka, R. (1986). Studies on the Tissue Culture of Stevia rebaudiana and Its Components; (II)1. Induction of Shoot Primordia. Planta Med, 52(4), 321-323.
Murashige T. and Skoog F. (1962). A revised medium for rapid growth and bioassays with tobacco cultures. Physiology Plant, 15, 473-497.
Thobunluepop, P. (2009). The somatic embryogenesis and plant regeneration from immature embryo of sweet corn inbred line. Journal of Plant Breeding and Crop Science, 1(10), 330-335.
Wongsansri, J., Chotikadachanarong, K., Phichai, K. and Nontaswatsr, C. (2015) Effects of Sodium alginate on Artificial Seed Production of Stevia rebaudiana Bertoni. In Phayao Research Conference 2015. (pp. 809-817). University Of Phayao: Phayao. (in Thai)
Chotikadachanarong K. and Dheeranupattana S. (2013). Micropropagation and acclimatization of Stevia rebaudiana Bertoni. Pakistan Journal of Biological Sciences, 16(7), 887-890.
Hudi, A. K. M. N., Rahman, M. and Bari, M. A. (2007). Effect of carbon source in alginate bead on synthetic seed germination in eggplant (Solanum melongena L.). Journal of Plant Sciences, 2(5), 538-544.
Manoj, K. R., Jaiswal, V.S. and Jaiswal, U. (2008). Effect of ABA and sucrose on germination of encapsulated somatic embryos of guava (Psidium guajava L.) Scientia Horticulturae, 117, 302–305.
Maqsood, M., Mujib, A. and Khusrau, M. (2015). Preparation and low temperature short-term storage for synthetic seeds of Caladium bicolor. Not Sci Biol, 7(1), 90-95.
Miyagawa, H., Fujioka, N., Kohda, H., Yamasaki, K., Taniguchi, K. and Tanaka, R. (1986). Studies on the Tissue Culture of Stevia rebaudiana and Its Components; (II)1. Induction of Shoot Primordia. Planta Med, 52(4), 321-323.
Murashige T. and Skoog F. (1962). A revised medium for rapid growth and bioassays with tobacco cultures. Physiology Plant, 15, 473-497.
Thobunluepop, P. (2009). The somatic embryogenesis and plant regeneration from immature embryo of sweet corn inbred line. Journal of Plant Breeding and Crop Science, 1(10), 330-335.
Wongsansri, J., Chotikadachanarong, K., Phichai, K. and Nontaswatsr, C. (2015) Effects of Sodium alginate on Artificial Seed Production of Stevia rebaudiana Bertoni. In Phayao Research Conference 2015. (pp. 809-817). University Of Phayao: Phayao. (in Thai)
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2018-01-25
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