Harvesting Efficiency of Chlorella sp. TISTR8263 Biomass by Magnetic Particles Synthesized from Co-Precipitation and Microwave Assisted Synthesis
Abstract
Harvesting efficiencies of Chlorella sp. TISTR8263 biomass by magnetic particles synthesized from co-precipitation (Magnetic nanoparticles: MNPs) and microwave assisted synthesis (Iron oxide magnetic microparticles: IOMMs) were compared. A dose of 200 - 800 mg/L MNPs and IOMMs were used as agents to separate 1g/L Chlorella sp. TISTR8263 cells from BG-11 medium at pH 9.1 ± 0.5. Most of MNPs particles were smaller in size than IOMMs. Size distribution analysis showed that 41.17% of MNPs particles ranged from > 5.0-10.0 mm, whereas 34.83% of IOMMs ranged from > 10.0-20.0 mm. In addition, MNPs showed a significantly higher efficiency in algal cell harvesting than IOMMs (p=0.023). Doses of used agents significantly effected on effectiveness of cell separation (p<0.001). There was a significantly interaction between used doses and magnetic particle products (p<0.001). At 800 mg/L, MNPs showed higher harvesting efficiency (95.30 ± 0.44 % or 1.906 ± 0.009 g algae/ g MNPs) than IOMMs (91.55 ± 0.39 % or (1.831 ± 0.008 g algae/ g IOMMs)). The results indicated that MNPs synthesized by co-precipitation exhibited higher efficiency in algal cell harvesting than IOMMs created from microwave assisted synthesis. Therefore, MNPs was selected as a core agent to further develop a new composite for harvesting algal cells. Keywords: magnetic particles, magnetic synthesis, harvesting efficiency, microalgaeReferences
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Vandamme, D., Foubert, I., Meesschaert, B., & Muylaertu, K. (2010). Flocculation of microalgae using cationic starch. Journal of Applied Phycology, 22, 525-530.
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A review. Renewable and Sustainable Energy Reviews, 41, 1489-1500.
Boli, E., Savvidou, M., Logothesis, D., Louli, V., Pappa, G., Voutsas, E., Kolosis, F., & Magoulas, K. (2017). Magnetic harvesting of marine algae Nannochloropsis oceanica. Separation Science and Technology, 1-8.
Cerff, M., Morweiser, M., Dillschneider, R., Michel, A., Menzel, K., & Posten, C. (2012). Harvesting fresh water and marine algae by magnetic separation: Screening of separation parameters and high gradient magnetic filtration. Bioresource Technology, 118, 289-295.
Chen, Y.M., Liu, J.C., & Ju, Y.H. (1998). Flotation removal of algae from water. Colloids and Surfaces B: Biointerfaces, 12, 49-55.
Chittapun, S., Limbipichai, S., Amnuaysin, N., Boonkerd, R., & Charoensook, M. (2017). Effects of using cyanobacteria and fertilizer on growth and yield of rice, Pathum Thani I: a pot experiment. Journal of Applied Phycology.
Chu, W.L. (2012). Biotechnological application of microalgae. IeJSME, 6, 24-37.
Girma, E., Belarbi, E.H., Fernandez, G.A., Medina, A.R., & Chisti, Y. (2003). Recovery of microalgal biomass and metabolites: process options and economics. Biotechnology Advances, 20, 491–515.
Hu, Y.R., Wang, F., Wang, S.K., Liu, C.Z., & Guo, C. (2013). Efficient harvesting of marine microalgae Nannochloropsis maritima using magnetic nanoparticles. Bioresource Technology, 136, 387-390.
Khan, K., Rehman, S., Rahman, H. U., & Khan, Q. (2014). Synthesis and application of magnetic nanoparticles. In J.M. Gonzalez Estevez (Ed.), Nanomagnetism. (pp. 135-159). Manchester: One Central Press (OCP).
Laurent, S., Forge, D., Port, M., Roch, A., Robic, C., Elst, L.C., & Muller, R.N. (2008). Magnetic Iron oxide Nanoparticles: Synthesis, Stabilization, Vectorization, Physicochemical Characterizations, and biological Application. Chemical Reviews, 108, 2064-2110.
Lekshmi, B., Rebekah, S.J., Anitta Jose, S., Abinandan, S., & Shanthakumar, S. (2015). Studies on reduction of inorganic pollutants from wastewater by Chlorella pyrenoidosa and Scenedesmus abundans. Alexandria Engineering Journal, 54, 1291-1296.
Mikhaylova, M., Kim, D.K., Bobrysheva, N., Osmolowsky, M., & Semenov, V. (2004). Superparamagnetism of magnetite nanoparticles: dependence on surface modification. Langmuir, 20(6), 2472-2477.
Pragya, N., Pandey, K.K., & Sahoo, P.K. (2013). A review on harvesting, oil extraction and biofuels production technologies from microalgae. Renewable and Sustainable Energy Reviews, 24, 159-71.
Prochazkova, G., Safarik, I., & Branyik, T. (2013). Harvesting microalgae with microwave synthesized magnetic microparticles. Bioresource Technology, 130, 472-477.
Safi, C., Zebib, B., Merah, O., Pontalier, P., & Vaca-Garcia, C. (2014). Morphology composition production processing and applications of Chlorella vulgaris: A review. Renewable and Sustainable Energy Reviews, 35, 265-278.
Saker, S., & Chandramohan, M. (2008). Phycobiliproteins as a commodity: trends in applied research, patents and commercialization. Journal of Applied Phycology, 20, 113–136.
Salopek, B., Krasic, D., & Filipovic, S. (1992). Measurement and application of zeta-potential. Rudarsko-geolosko- naftni zbornik, 4, 147-151.
Uduman, N., Qi, Y., Danquah, M.K., Forde, G.M., & Hoadley, A. (2010). Dewatering of microalgal cultures: A major bottleneck to algae-based fuels. Journal of Renewable and Sustainable Energy, 2, 012701.
Vandamme, D., Foubert, I., Meesschaert, B., & Muylaertu, K. (2010). Flocculation of microalgae using cationic starch. Journal of Applied Phycology, 22, 525-530.
Wang, S.K., Stiles, A.R., Guo, C., & Liu, C.Z. (2015). Harvesting microalgae by magnetic separation: A review. Algal Research, 9, 178-85.
Xu, L., Guo, C., Wang, F., Zheng, S., & Liu, C.Z. (2011). A simple and rapid harvesting method for microalgae by in situ magnetic separation. Bioresource Technology, 102, 10047-10051.
Zhao, Y., Liang, W., Liu, L., Li, F., Fan, Q., & Sun, X. (2015). Harvesting chlorella vulgaris by magnetic flocculation using Fe3O4 coating with polyaluminium chloride and polyacrylamide. Bioresource Technology, 198, 789-796.
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2018-02-08
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