Possibility of Soil Mangrove-Derived Oleaginous Yeast Rhodotorula mucilaginosa on Nutritionally Optimized Medium for Lipid Production for Alternative Biodiesel Feedstock
Abstract
Lipid-derived from oleaginous yeast is now being considered as promising for biodiesel feedstock. In this study, oleaginous yeasts were screened and isolated from soil mangrove samples collected in the eastern region of Thailand. A total of fifty one isolates were obtained, and preliminarily examined as potential lipid producer by cultivating in a nitrogen-limiting medium containing 50 g/L of glucose as a sole carbon source. Only twenty three isolates were observed the accumulation of intracellular lipid granule. It was found that the yeast strain SMY40 accumulated the highest lipid content 29.44% of their dry biomass weight, which was defined as oleaginous yeast and identified to be a Rhodotorula mucilaginosa. Optimization of nutritional parameters and culture condition were carried out to improved biomass and lipid production of R. mucilaginosa SMY40.Under the optimal condition; 1.67 g/L YNB w/o aa-AS with initial pH of 4.7 containing 70 g/L glucose and 10.0 g/L urea, incubation temperature at 30°C, orbital shaking speed at 150 rpm for 120 h of cultivation time, 4.40 g/L of dry biomass, 1.76 g/L of cellular lipid accumulation and up to 40.00%of accumulated lipid of total dry biomass weight were produced. The produced lipid composition from R. mucilaginosa SMY40 contained the high proportion of C16 and C18 fatty acids. The extracted lipids were mainly 20.21% palmitic acid (C16:0), 2.40% palmitoleic acid (C16:1), 5.85% stearic acid (C18:0), 51.64% oleic acid (C18:1) and 12.17% linoleic acid (C18:2) thatiscomparable to conventional vegetable oils. The results suggest that the extracted lipids of R. mucilaginosa SMY40 could be used as feedstock for biodiesel production.Keywords: Rhodotorula mucilaginosa, oleaginous yeast, soil mangrove, extracted lipid, biodiesel feedstockReferences
Chang, Y. H., Chang, K. S., Hsu, C. L., Chuang, L. T., Chen, C. Y., Huang, F. U. and Jang, H. D. (2013). A comparative study on batch and fed-batch cultures of oleaginous yeast Cryptococcus sp. in glucose-based media and corncob hydrolysate for microbial oil production. Fuel, 105(1), 711-717.
Dasgupta, D., Sharma, T., Bhatt, A., Bandhu, S. and Ghosh, D. (2017). Cultivation of oleaginous yeast Rhodotorula mucilaginosa IIPL32 in split column airlift reactor and its influence on fuel properties. Biocatalysis and Agricultural Biotechnology, 10, 308-316.
Evan, C. T. and Ratledge, C. (1984). Influence of nitrogen metabolism on lipid accumulation in oleaginous yeasts. Journal of General Microbiology, 130(7), 1693-1704.
Evans, E. T., Scragg, A. H. and Ratledge, C. (1981). Regulation of citrate efflux from mitochondria of oleaginous and non-oleaginous yeasts by adenine nucleotides. European Journal of Biochemistry, 130(1), 609-615.
Gong, Z. W., Shen, H. W., Wang, Q., Yang, X. B., Xie, H. B. and Zhao, Z. B. K. (2013). Efficient conversion of biomass into lipids by using the simultaneous saccharification and enhance lipid production process. Biotechnology and Biofuels, 6, 36.
Han, X., Miao X. L. and Wu, Q. Y. (2006). High quality biodiesel production from heterotrophic growth of Chlorella protothcoides in fermenters by using starch hydrolysate as organic carbon. Journal of Biotechnology,126(4), 499-507.
Huang, J. Z., Shi, Q. Q., Zhou, X. L., Lin, Y. X., Xie, B. F. and Wu, S. G. (1998). Studies on the breeding of Mortierella isabellina mutant high producing lipid and its fermentation conditions. Microbiology, 25(4),
187-191.
Kitcha, S. and Cheirsilp, B. (2011). Screening of oleaginous yeasts and optimization for lipid production using crude glycerol as a carbon source. Energy Procedia, 9, 274-282.
Kurtzman, C. P. and Fell, J. W. (2000). The yeasts. A Taxonomic study. (4th revised and enlarged edition).
(pp 367-370). Amsterdum, Lausanne, New York, Oxford, Shannon, Singapore, Tokyo: Elsevier.
Kurtzman, C. P. and Robonett, C. J. (1998). Identification and phylogeny of ascomycetous yeasts from analysis of nuclear large subunit (26S) ribosomal DNA partial sequences. Antonie van Leeuwenhoek Journal, 73(4), 331-371
Leesing, R. and Nantaso, N. (2011). Isolation and cultivation of oleaginous yeast for microbial oil production. KKU Research Journal, 16(2), 112-126.
Li, M., Liu, G. L., Chi, Z. and Chi, Z. M. (2010). Single cell oil production from hydrolysate of cassava starch by marine-derived yeast Rhodotorula mucilaginosa TYJ15a. Biomass and Bioenergy, 34(1), 101-107.
Li, S. L., Lin, Q., Li X. R., Xu, H., Yang, Y. X., Qiao, D. R. and Cao, Y. (2012). Biodiversity of the oleaginous microorganism in Tibetan PlaTeau. Brazilian Journal of Microbiology, 43(2), 627-634.
Li, X. P., Deng, F. Y., Li, S., Wei, L., Gui, G. C. andZhi, Q. L. (2009). Isolation of the oleaginous yeasts from the soil and studies of their lipid-producing capacities. Food Technology and Biotechnology, 47(2), 215-220.
Meng, X., Yang, J., Xua, X., Zhang, L., Nie,Q.andXian, M. (2009). Biodiesel production from oleaginous microorganisms. Renewable Energy, 34(1), 1-5.
Miller, G. L. (1959). Use of dinitrosalicylic acid reagent for determination of reducing sugar. Annual Chemistry, 31(3), 426-428.
Moser, B. R. (2008). Influence of blending canola, palm, soybean and sunflower oil methyl esters on fuel properties of biodiesel. Energy Fuel, 22(6), 4301-4306.
Pan, L. X., Yang, D. F., Shao, L., Li., W., Chen, G. G. and Liang, Z. Q. (2009). Isolation of the oleaginous yeasts from the soil and studies of their lipid-producing capacities. Food Technology and Biotechnology, 47(2), 215-220.
Papanikolaou, S., Komaitis, M. and Aggelis G. (2004). Single cell oil (SCO) production by Mortierella isabellina grown on high-sugar content media. Bioresource Technology, 95(3), 287-291.
Ramos, M. J., Fernandez, C. M., Casas, A., Rodriguez, L. and Perez, A. (2009). Influence of fatty acid composition of raw materials on biodiesel properties. Bioresource Technology, 100(1), 261-268.
Sambrook, J., Fritsch, E. F. and Maniatis, T. (1989). Preparation and analysis of eukaryotic genomic DNA.
In: Molecular cloning: a laboratory manual.(2ndedition). (pp 367-370). Bejing:ColdSpringHabor Laboratory Press. (Chinese Translating Ed.).
Sriwongchai, S., Pokethitiyook,P., Kruatrachue, M., Bajwa, P. K. and Lee, H. (2013). Screening of selected oleaginous yeasts for lipid production from glycerol and some factors which affect lipid production by Yarrowia lipolyticastrains. Journalof Microbiology, Biotechnology and Food Sciences, 2(5), 2344-2348.
Sriwongchai S., Pokethitiyook, P., Pugkaew, W., Kruatrachue, M. and Lee, H. (2012). Optimization of lipid production in the oleaginous bacterium Rhodococcuserythropolisgrowing on glycerol as the sole carbon source. African Journal of Biotechnology, 11(79), 14440-14447.
Tanimura, A., Takashima, M., Sugita, T., Endoh, T., Kikukawa, M., Yamaguchi, S., Sakuradani, E., Ogawa, J. and Shima, J. (2014). Selection of oleaginous yeasts with high lipid productivity for practical biodiesel production. Bioresource Technology, 153, 230-235.
Thakur, M. S., Prapulla, S. G. and Karanth, N. G. (1989). Estimation of intracellular lipids by the measurement of absorbance of yeast cells stained with Sudan Black B. Enzyme and Microbial Technology, 11(4),
252-254.
Thiru, M., Sankh, S. and Rangaswamy, V. (2011). Process gor biodiesel from Cryptococcus curvatus. Bioresource Technology, 102(22), 10436-10440.
Wang, C. L., Li, Y., Xin, F. H., Liu, Y. Y. and Chi, Z. M. (2014). Evaluation of single cell oil from Aureobasidium pullulans var. melanogenum P10 isolated from mangrove ecosystems for biodiesel production. Process Biochemistry, 49(5), 725-731.
Wang, L., Yue, L. X., Chi, Z. M. and Wang, X. H.(2008). Marine killer yeasts active against the pathogenic yeast strain in crab (Portunus trituberculatus). Diseases of Aquatic Organisms, 80, 211-218.
Xue, F.,Miao,J., Zhang, X.,Luo,H. and Tan,T. (2008). Studies on lipid production by Rhodotorula glutinis fermentation using monosodium glutamate wastewater as culture medium. Bioresource Technology, 99(13), 5923-5927.
Yu, X., Zheng, Y., Xiong, X. and Chen, S. (2014). Co-utilization of glucose, xylose and cellobiose by the oleaginous yeast Crytococcus curvatus. Biomass and Bioenergy, 71, 340-349.
Zhang, J., Fang, X., Zhu, X. L., Li, Y. Xu, H. P., Zhao, B. F. et al. (2011). Microbial lipid production by the oleaginous yeast Cryptococcus curvatus O3 grown in fed-batch culture. Bioresource Technology, 35(5), 1906-1911.
Zhu, L. Y., Zong, M. H. and Wu, H. (2008). Efficient lipid production with Trichosporon fermentansand its use for
Dasgupta, D., Sharma, T., Bhatt, A., Bandhu, S. and Ghosh, D. (2017). Cultivation of oleaginous yeast Rhodotorula mucilaginosa IIPL32 in split column airlift reactor and its influence on fuel properties. Biocatalysis and Agricultural Biotechnology, 10, 308-316.
Evan, C. T. and Ratledge, C. (1984). Influence of nitrogen metabolism on lipid accumulation in oleaginous yeasts. Journal of General Microbiology, 130(7), 1693-1704.
Evans, E. T., Scragg, A. H. and Ratledge, C. (1981). Regulation of citrate efflux from mitochondria of oleaginous and non-oleaginous yeasts by adenine nucleotides. European Journal of Biochemistry, 130(1), 609-615.
Gong, Z. W., Shen, H. W., Wang, Q., Yang, X. B., Xie, H. B. and Zhao, Z. B. K. (2013). Efficient conversion of biomass into lipids by using the simultaneous saccharification and enhance lipid production process. Biotechnology and Biofuels, 6, 36.
Han, X., Miao X. L. and Wu, Q. Y. (2006). High quality biodiesel production from heterotrophic growth of Chlorella protothcoides in fermenters by using starch hydrolysate as organic carbon. Journal of Biotechnology,126(4), 499-507.
Huang, J. Z., Shi, Q. Q., Zhou, X. L., Lin, Y. X., Xie, B. F. and Wu, S. G. (1998). Studies on the breeding of Mortierella isabellina mutant high producing lipid and its fermentation conditions. Microbiology, 25(4),
187-191.
Kitcha, S. and Cheirsilp, B. (2011). Screening of oleaginous yeasts and optimization for lipid production using crude glycerol as a carbon source. Energy Procedia, 9, 274-282.
Kurtzman, C. P. and Fell, J. W. (2000). The yeasts. A Taxonomic study. (4th revised and enlarged edition).
(pp 367-370). Amsterdum, Lausanne, New York, Oxford, Shannon, Singapore, Tokyo: Elsevier.
Kurtzman, C. P. and Robonett, C. J. (1998). Identification and phylogeny of ascomycetous yeasts from analysis of nuclear large subunit (26S) ribosomal DNA partial sequences. Antonie van Leeuwenhoek Journal, 73(4), 331-371
Leesing, R. and Nantaso, N. (2011). Isolation and cultivation of oleaginous yeast for microbial oil production. KKU Research Journal, 16(2), 112-126.
Li, M., Liu, G. L., Chi, Z. and Chi, Z. M. (2010). Single cell oil production from hydrolysate of cassava starch by marine-derived yeast Rhodotorula mucilaginosa TYJ15a. Biomass and Bioenergy, 34(1), 101-107.
Li, S. L., Lin, Q., Li X. R., Xu, H., Yang, Y. X., Qiao, D. R. and Cao, Y. (2012). Biodiversity of the oleaginous microorganism in Tibetan PlaTeau. Brazilian Journal of Microbiology, 43(2), 627-634.
Li, X. P., Deng, F. Y., Li, S., Wei, L., Gui, G. C. andZhi, Q. L. (2009). Isolation of the oleaginous yeasts from the soil and studies of their lipid-producing capacities. Food Technology and Biotechnology, 47(2), 215-220.
Meng, X., Yang, J., Xua, X., Zhang, L., Nie,Q.andXian, M. (2009). Biodiesel production from oleaginous microorganisms. Renewable Energy, 34(1), 1-5.
Miller, G. L. (1959). Use of dinitrosalicylic acid reagent for determination of reducing sugar. Annual Chemistry, 31(3), 426-428.
Moser, B. R. (2008). Influence of blending canola, palm, soybean and sunflower oil methyl esters on fuel properties of biodiesel. Energy Fuel, 22(6), 4301-4306.
Pan, L. X., Yang, D. F., Shao, L., Li., W., Chen, G. G. and Liang, Z. Q. (2009). Isolation of the oleaginous yeasts from the soil and studies of their lipid-producing capacities. Food Technology and Biotechnology, 47(2), 215-220.
Papanikolaou, S., Komaitis, M. and Aggelis G. (2004). Single cell oil (SCO) production by Mortierella isabellina grown on high-sugar content media. Bioresource Technology, 95(3), 287-291.
Ramos, M. J., Fernandez, C. M., Casas, A., Rodriguez, L. and Perez, A. (2009). Influence of fatty acid composition of raw materials on biodiesel properties. Bioresource Technology, 100(1), 261-268.
Sambrook, J., Fritsch, E. F. and Maniatis, T. (1989). Preparation and analysis of eukaryotic genomic DNA.
In: Molecular cloning: a laboratory manual.(2ndedition). (pp 367-370). Bejing:ColdSpringHabor Laboratory Press. (Chinese Translating Ed.).
Sriwongchai, S., Pokethitiyook,P., Kruatrachue, M., Bajwa, P. K. and Lee, H. (2013). Screening of selected oleaginous yeasts for lipid production from glycerol and some factors which affect lipid production by Yarrowia lipolyticastrains. Journalof Microbiology, Biotechnology and Food Sciences, 2(5), 2344-2348.
Sriwongchai S., Pokethitiyook, P., Pugkaew, W., Kruatrachue, M. and Lee, H. (2012). Optimization of lipid production in the oleaginous bacterium Rhodococcuserythropolisgrowing on glycerol as the sole carbon source. African Journal of Biotechnology, 11(79), 14440-14447.
Tanimura, A., Takashima, M., Sugita, T., Endoh, T., Kikukawa, M., Yamaguchi, S., Sakuradani, E., Ogawa, J. and Shima, J. (2014). Selection of oleaginous yeasts with high lipid productivity for practical biodiesel production. Bioresource Technology, 153, 230-235.
Thakur, M. S., Prapulla, S. G. and Karanth, N. G. (1989). Estimation of intracellular lipids by the measurement of absorbance of yeast cells stained with Sudan Black B. Enzyme and Microbial Technology, 11(4),
252-254.
Thiru, M., Sankh, S. and Rangaswamy, V. (2011). Process gor biodiesel from Cryptococcus curvatus. Bioresource Technology, 102(22), 10436-10440.
Wang, C. L., Li, Y., Xin, F. H., Liu, Y. Y. and Chi, Z. M. (2014). Evaluation of single cell oil from Aureobasidium pullulans var. melanogenum P10 isolated from mangrove ecosystems for biodiesel production. Process Biochemistry, 49(5), 725-731.
Wang, L., Yue, L. X., Chi, Z. M. and Wang, X. H.(2008). Marine killer yeasts active against the pathogenic yeast strain in crab (Portunus trituberculatus). Diseases of Aquatic Organisms, 80, 211-218.
Xue, F.,Miao,J., Zhang, X.,Luo,H. and Tan,T. (2008). Studies on lipid production by Rhodotorula glutinis fermentation using monosodium glutamate wastewater as culture medium. Bioresource Technology, 99(13), 5923-5927.
Yu, X., Zheng, Y., Xiong, X. and Chen, S. (2014). Co-utilization of glucose, xylose and cellobiose by the oleaginous yeast Crytococcus curvatus. Biomass and Bioenergy, 71, 340-349.
Zhang, J., Fang, X., Zhu, X. L., Li, Y. Xu, H. P., Zhao, B. F. et al. (2011). Microbial lipid production by the oleaginous yeast Cryptococcus curvatus O3 grown in fed-batch culture. Bioresource Technology, 35(5), 1906-1911.
Zhu, L. Y., Zong, M. H. and Wu, H. (2008). Efficient lipid production with Trichosporon fermentansand its use for
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2018-02-08
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