Screening of Phylloplane Bacteria from Tomato Leaves to Control Stemphylium sp., a Causal Agent of Gray Leaf Spot Disease
Abstract
This research aims to screening phylloplane bacteria from tomato leaves to control Stemphylium sp., a causal agent of gray leaf spot disease. Forty six isolates were obtained including 40 isolates via leaf washing and 6 isolates via leaf printing technique. All of isolates were tested in vitro for growth inhibitory effect against Stemphylium sp. on potato dextrose agar (PDA) using dual culture technique. Among these phylloplane bacteria, 6 isolates demonstrated inhibitory effect against the growth of Stemphylium sp. with % inhibition in range of 47.43-58.36%. Then, the six potent bacteria were tested for the efficacy to control gray leaf spot disease by detached leaf technique. The result showed that PDN15 was the most effective to reduce the lesion sizes by 78.50% as compared to non-bacteria treated. Base on morphological and biochemical testing, PDN15 was identified as Bacillus sp. Keywords : Stemphylium sp., phylloplane, gray leaf spot diseaseาReferences
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Hussein, M.A.M., Hassan, M.H.A., Allam, A.D.A. & Abo-Elyousr, K.A.M. (2007). Management of Stemphylium blight of onion by using biological agents and resistance inducers. Egyptian Journal of Phytopathology, 35(1), 49-60.
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Nainwal, D. & Vishunavat, K. (2016). Management of purple blotch and Stemphylium blignt of onion in Tarai and Bhabar regions of Uttarakhand, India. Journal of Applied and Natural Science, 8(1), 150-153.
Nasehi, A., Kadir, J.B., Zainal, A.M.A., Wong, M.Y. & Abed, A.F. (2012). First report of gray leaf spot on pepper caused by Stemphylium solani in Malaysia. Plant disease, 96(8), 1227.
Sariah, M. (1994). Potential of Bacillus spp. as biocontrol agent for anthracnose fruit rot of chilli. Malaysian Applied Biology Journal, 23(2), 53-60.
Sutthisa, W. (2002). Screening and application of phylloplane microorganisms to control Alternaria brassicicola, a causal agent of leaf spot on Chinese kale. Master Thesis. Department of plant pathology, Faculty of Agriculture, Kasetsart University Thailand. (in Thai)., 88p.
Sutthisa, W., Tapkhumram, P., Kanchanarat, W. & Arimastu, P. (2014). Efficiency of Thai medicinal plant extract to control Colletotrichum sp., a causal agent of mango anthracnose. Khon Kaen Agricultural Journal, 42 Supplement (1), 665-670.
Thummabenjapone, P. & Phola, S. (2007). A highly potential fungicide to control Stemphylium sp., a causal agent of gray spot of tomato. In Proceeding The 8th National Plant Protection Conference.
(pp. 383-391). Phitsanulok: Thailand.
Williams, R.H. (1994). Bergey’s Manual of Determinative Bacteriology. USA, Baltimore.
Hussein, M.A.M., Hassan, M.H.A., Allam, A.D.A. & Abo-Elyousr, K.A.M. (2007). Management of Stemphylium blight of onion by using biological agents and resistance inducers. Egyptian Journal of Phytopathology, 35(1), 49-60.
Inwang, B. (1987). Control of Rhizoctonia solani KUEHN by microorganisms selected from agricultural soils. Master Thesis. Department of plant pathology, Faculty of Agriculture, Kasetsart University Thailand. (in Thai).
Jareanruk, P. (2007). Applicationof antagonistic bacteria for the control of tomato black leaf mold caused by Pseudocercospora fuligena (Roldan) Deighton. Master Thesis. Department of plant pathology, Faculty of Agriculture, Kasetsart University Thailand. (in Thai).
Montesinos, E., Bonaterra, A., Ophir, Y. & Beer, S.V. (1996). Antagonism of selected bacterial strains to Stemphylium vesicarium and biological control of brown spot of pear under controlled environment conditions. Biological Control, 86(8), 856-863.
Nainwal, D. & Vishunavat, K. (2016). Management of purple blotch and Stemphylium blignt of onion in Tarai and Bhabar regions of Uttarakhand, India. Journal of Applied and Natural Science, 8(1), 150-153.
Nasehi, A., Kadir, J.B., Zainal, A.M.A., Wong, M.Y. & Abed, A.F. (2012). First report of gray leaf spot on pepper caused by Stemphylium solani in Malaysia. Plant disease, 96(8), 1227.
Sariah, M. (1994). Potential of Bacillus spp. as biocontrol agent for anthracnose fruit rot of chilli. Malaysian Applied Biology Journal, 23(2), 53-60.
Sutthisa, W. (2002). Screening and application of phylloplane microorganisms to control Alternaria brassicicola, a causal agent of leaf spot on Chinese kale. Master Thesis. Department of plant pathology, Faculty of Agriculture, Kasetsart University Thailand. (in Thai)., 88p.
Sutthisa, W., Tapkhumram, P., Kanchanarat, W. & Arimastu, P. (2014). Efficiency of Thai medicinal plant extract to control Colletotrichum sp., a causal agent of mango anthracnose. Khon Kaen Agricultural Journal, 42 Supplement (1), 665-670.
Thummabenjapone, P. & Phola, S. (2007). A highly potential fungicide to control Stemphylium sp., a causal agent of gray spot of tomato. In Proceeding The 8th National Plant Protection Conference.
(pp. 383-391). Phitsanulok: Thailand.
Williams, R.H. (1994). Bergey’s Manual of Determinative Bacteriology. USA, Baltimore.
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2017-07-12
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บทความวิจัยจากการประชุมวิชาการระดับชาติ"วิทยาศาสตร์วิจัย"ครั้งที่ 9
