No Nucleotide Sequence Variation at Non-Coding Mitochondrial DNA of Cassava (Manihot esculenta Crantz)
Abstract
Cassava (Manihot esculenta Crantz) is an important economic crop of Thailand. The purpose of this study is to investigate the non-coding mitochondrial DNA region of cassava that has not yet been reported for use as a molecular marker for cultivar identification. In this study, 18 cassava cultivars were used to obtain the 368-bp segment using trn-Phe and trn-F primers in the Polymerase Chain Reaction. The non-coding mitochondrial DNA region of all samples were 268 base pairs, with no nucleotide difference. Comparison between sequences of other members within Euphorbiaceous (same family as cassava), Para rubber (Hevea brasiliensis) and castor bean (Ricinus communis), revealed 10 and 33 base pairs being different, respectively. Moreover, when compared to mung bean (Vigna radiata (L.)), out of Euphorbiaceous family, found 32 base pairs different. Therefore, the non-coding mitochondrial DNA region is not suitable for cassava identification at the cultivar level. Keywords : DNA, cassava, mitochondrial DNA, PCRReferences
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Their Suitability as DNA Barcodes for Identification of Plants in the Genus Alpinia Roxb. (Zingiberaceae). The 12th National Graduate Research Conference, 552-563.
Pring, D.R., & Lonsdale, D.M. (1985). Moleccular Biology of Higher Plant Mitochondrial DNA. International Review of Cytology, 97, 1-46.
Ward, B.L., Anderson, R.S., & Bendich, A.J. (1981). The Mitochondrial Genome is Large and Variable in a Family of Plants (Cucurbitaceae). Cell, 25, 793-803.
Wongtiem, P. (2012). Morphological Studies of Genetic Resources of Ex Situ and In Situ Cassava. Rayong Field Crops Research Center. Department of Agriculture. Thailand.
Archak, S. (2000). Plant DNA Fingerprinting: An Overview. Ag Biotech Net, 2, 1-6.
Christensen, A.C. (2014). Plant Mitochondrial Genome Evolution can be Explained by DNA Repair Mechanisms.Genome Biology and Evolution, 5(6), 1079-1086.
Duangjit, S. (2011). Mitochondrial DNA and Forensic Application. Forensic Medicine Journal, 4(1), 53-65.
Jeffrey, D.P., & Laura, A.H. (1988). Plant Mitochondrial DNA Evolves Rapidly in Structure, but Slowly in Sequence. Journal of Molecular Evolution, 28, 87-97.
Kress, J.W., & Erickson, D.L. (2008). DNA Barcodes: Genes, Genomics, and Bioinformatics. Proceedings of
the National Academy of Sciences, 105, 2761-2762.
Lebot, V. (2009). The Tropical Root and Tuber Crops: Cassava, Sweet Potato, Yams and Aroids. The Centre
for Agriculture and Bioscience International, 17, 143.
Palmer, J.D. (1985). Evolution of Chloroplast and Mitochondrial DNA in Plants and Algae. Plenum,131-240.
Petcha, N., Maensiri, D., & Saensouk, S. (2016). Assessment of rpoB and rpoC1 Plastid DNA Regions for
Their Suitability as DNA Barcodes for Identification of Plants in the Genus Alpinia Roxb. (Zingiberaceae). The 12th National Graduate Research Conference, 552-563.
Pring, D.R., & Lonsdale, D.M. (1985). Moleccular Biology of Higher Plant Mitochondrial DNA. International Review of Cytology, 97, 1-46.
Ward, B.L., Anderson, R.S., & Bendich, A.J. (1981). The Mitochondrial Genome is Large and Variable in a Family of Plants (Cucurbitaceae). Cell, 25, 793-803.
Wongtiem, P. (2012). Morphological Studies of Genetic Resources of Ex Situ and In Situ Cassava. Rayong Field Crops Research Center. Department of Agriculture. Thailand.
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2017-08-22
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บทความวิจัยจากการประชุมวิชาการระดับชาติ"วิทยาศาสตร์วิจัย"ครั้งที่ 9
