Effects of Culture Media and Plant Growth Regulators on In Vitro Seedling Growth of Pholidota imbricata Lindl.
Abstract
The effect of different media (MS, ½MS, VW and ½VW) on growth of in vitro seedling culture of Pholidota imbricata Lindl. was studied for 8 weeks. The results showed that the highest average of shoot numbers (1.68 shoots/plant) (47.5%), leaf numbers (5.18 leaves/plant) and root numbers (7.60 roots/plant) (100%) and shoot length (3.81 cm) were found in plantlet culture on the ½VW medium. Moreover, effect of different types and concentrations of cytokinins (BA, kinetin, TDZ) on in vitro seedling culture of P. imbricata Lindl. on ½VW medium for 8 weeks was also investigated. The results showed that the highest percentage of pseudobulb formation (30%), shoot numbers (1.68 shoots/plant) (60%) and leaf numbers (4.25 leaves/plant) were obtained when cultured on the medium with 0.1 mg/L TDZ. The seedling culture of P. imbricata Lindl. on ½VW medium supplemented with different types and concentrations of auxins (NAA, IAA, IBA) for 8 weeks demonstrated that highest average of shoot numbers (1.63 shoots/plant), the percentage of shoot formation (32.5%) and leaf numbers (6.55 leaves/plant) were observed when cultured on the medium with 0.1 mg/L IAA. Keyword: Pholidota imbricata Lindl., plant growth regulators, media, in vitroReferences
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Choopeng, S. and Musoryaena, N. (2014). Effects of PBZ, BA and TDZ on growth and development of Calanthe vestita Lindl. in vitro. Songklanakarin Journal of Plant Science, 1(2), 35 – 38. (in Thai)
Dasri, K., Munglue, P., Rattana, K. and Sangchanjiradet, S. (2016). The effects of IAA produced by Bacillus pumilus A1_YM_1 on growth of orchids under micropropagation. Khon Kaen Agriculture Journal, 44(1), 832 – 837.
Duncan, D.B. (1955). Multiple range and multiple F test. Biometrics, 11, 1 – 42.
Fangmuang, W. and Kongbangkerd, A. (2012). Effect of plant growth regulators on development of in vitro shoot culture of Dendrobium lamellatum Lindl. In Proceedings of the 1st Phayao Research Conference. (pp. 96 – 102). University of Phayao. (in Thai)
Giri, D. and Tamta, S. (2012). Propagation and conservation of Dactylorhiza hatagirea (D. Don) Soo, an endangered alpine orchid. African Journal of Biotechnology, 11(62), 12586 – 12594.
Hossain, M.M. (2008). Asymbiotic seed germination and in vitro seedling development of Epidendrum ibaguense Kunth. (Orchidaceae). African Journal of Biotechnology, 7(20), 3614 – 3619.
Hossain, M.M., Sharma, M, da Silva, J.A.T., Pathak, P. (2010). Seed germination and tissue culture of Cymbidium giganteum Wall. ex Lindl. Scientia Horticulturae, 123, 479 – 487.
Huetteman, C.A. and Preece, J.E. (1993). Thidiazuron a potent cytokinin for woody plant tissue culture.
Plant Cell, Tissue and Organ Culture, 33, 105 – 119.
Hutchinson, J.F., Beardsell, D.V. and Comb, J.A.M. (1985). Propagation by tissue culture introduction.
In Lamont, B.B. and Watkins, P.A. (eds). Horticulture of Australian Plants. Western Australian Dept. Agriculture, South Perth, W.A., 38 – 52.
Knudson, L. (1946). A new nutrient for the germination of orchid seeds. American Orchid Society Bulletin,
15, 214 – 217.
Kuljaroensub, V., Kunakhonnuruk, B. and Kongbangkerd, A. (2014). Effect of media and cytokinins on growth and development of in vitro culture of Panisea uniflora (Lindl.) Lindl. In Proceedings The 6th National Science Research Conference. (pp. 225 – 230). Burapha University. (in Thai)
Mary, S.S. and Divakar, K.M. (2015). In vitro propagation of epiphytic orchid Pholidota imbricata Hook. of Western Ghats. International Journal of Engineering Research & Technology, 4(10), 107 – 112.
Murashige, T. and Skoog, F. (1962). A revised medium for rapid growth and bioassay with tobacco tissue culture. Plant Physiology, 15, 473 – 497.
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Pierik, R.L.M., Sprenkels, P.A., Harst, B.V.D. and Meys, Q.G.V.D. (1988). Seed germination and further development of plantlets of Paphiopedilum ciliolare Pfitz. in vitro. Scientia Horticulturae, 34, 139-153.
Razdan, M.K. (2002). Introduction to plant tissue culture. (2nd ed.) USA: Science Publishers.
Ritti, W., Thapyai, C. and Kongbangkerd, A. (2013). Effect of cytokinins and auxins on morphological changes of in vitro rhizome tip culture of Eulophia siamensis Rolfe ex Downie. In Proceedings of the 5th Science Research Conference. (pp. 207 – 213). University of Phayao. (in Thai)
Roy, A.R., Patel, R.S., Patel, V.V., Sajeev, S. and Deka, B.C. (2011). Asymbiotic seed germination, mass propagation and seedling development of Vanda coerulea Griff ex. Lindl. (Blue Vanda) an in vitro protocol for an endangered orchid. Scientia Horticulturae, 128, 325 – 331.
Ruengverayut, S., Bhinija, K., Loprasert, S. and Huehne, P.S. (2005). Effect of cytokinin on multiple shoots induction in Coelogyne, Dendrobium and Vanda orchids. In Proceedings of 43rd Kasetsart University Annual Conference: Plants. (pp. 467 – 474). Kasetsart University. (in Thai)
Santarunai, N. (2015). Effect of medium and plant growth regulator on development and flowering (Dendrobium friedericksianum Rchb.f.) of orchid in vitro. In The National Conference & Research Presentation 2015 “Create and Development to Approach ASEAN Community II”. (pp. 155 – 162). Nakhon Ratchasima College. (in Thai)
Shadang, R., Dwivedi, P., Hegde, S.H. and Ahmed, N. (2007). Effects of different culture media on seed germination and subsequent in vitro development of protocorms of Hygrochilus parishii (Veith & Rchb.f.) Pfitz (Orchidaceae). Indian Journal of Biotechnology, 6, 256 – 261.
Sittisujjatham, S. (2006). Wild Orchid of Thailand (6th ed). Bangkok: Baan Lae Suan Printing. (in Thai)
Stewart, S.L. and Kane, M.E. (2006). Asymbiotic seed germination and in vitro seedling development of Habenaria macroceratitis (Orchidaceae), a rare Florida terrestrial orchid. Plant Cell, Tissue and Organ Culture, 86, 147 – 158.
Street, H.E. (1977). Plant tissue and cell culture. (2nd ed) California: University of California Press.
Supinrach, S and Supinrach, I. (2014). Effects of BA and NAA on in vitro growth of Dendrobium lindleyi Steud. seedlings. Burapha Science Journal, 19(2), 84 – 92. (in Thai)
Taji, A.M. and Williams, R.R. (1996). Tissue culture of Australian plants. Australia: University of New England Press.
Thaithong, O. (2008). Thai orchids. (15th.) Bangkok: Baan Lae Suan Printing. (in Thai)
Trigiano, R.N. and Gray, D.J. (2004). Plant development and biotechnology. USA: CRC Press LLC.
Vacin, E. and Went, F. (1949). Some pH changes in nutrient solution. Botanical Gazette, 110, 605 – 613.
Vaz, A.P. and Kerbuay, G.B. (2000). Effects of mineral nutrients on in vitro growth and flower formation of Psygmorchis pusilla (Orchidaceae). Acta Horticulture, 250, 149 – 156.
Vejsadova, H. (2006). Factors affecting seed germination and seedling growth of terrestrial orchids cultured
in vitro. Acta Biologica Cracoviensia Series Botanica, 48(1), 109 – 113.
Vejsadova, H., Latalova, K., and Rizkova, R. (2002). Influence of growth regulators on terrestrial orchid culture under in vitro conditions. Acta Pruhoniciana, 73, 27 – 36.
Wattanawikkit, P., Bunn, E., Chayanarit, K. and Tantiwiwat, S. (2011). Effect of cytokinins (BAP and TDZ) and auxin (2,4-D) on growth and development of Paphiopedilum callosum. Kasetsart Journal Natural Science, 45, 12 – 19.
Choopeng, S. and Musoryaena, N. (2014). Effects of PBZ, BA and TDZ on growth and development of Calanthe vestita Lindl. in vitro. Songklanakarin Journal of Plant Science, 1(2), 35 – 38. (in Thai)
Dasri, K., Munglue, P., Rattana, K. and Sangchanjiradet, S. (2016). The effects of IAA produced by Bacillus pumilus A1_YM_1 on growth of orchids under micropropagation. Khon Kaen Agriculture Journal, 44(1), 832 – 837.
Duncan, D.B. (1955). Multiple range and multiple F test. Biometrics, 11, 1 – 42.
Fangmuang, W. and Kongbangkerd, A. (2012). Effect of plant growth regulators on development of in vitro shoot culture of Dendrobium lamellatum Lindl. In Proceedings of the 1st Phayao Research Conference. (pp. 96 – 102). University of Phayao. (in Thai)
Giri, D. and Tamta, S. (2012). Propagation and conservation of Dactylorhiza hatagirea (D. Don) Soo, an endangered alpine orchid. African Journal of Biotechnology, 11(62), 12586 – 12594.
Hossain, M.M. (2008). Asymbiotic seed germination and in vitro seedling development of Epidendrum ibaguense Kunth. (Orchidaceae). African Journal of Biotechnology, 7(20), 3614 – 3619.
Hossain, M.M., Sharma, M, da Silva, J.A.T., Pathak, P. (2010). Seed germination and tissue culture of Cymbidium giganteum Wall. ex Lindl. Scientia Horticulturae, 123, 479 – 487.
Huetteman, C.A. and Preece, J.E. (1993). Thidiazuron a potent cytokinin for woody plant tissue culture.
Plant Cell, Tissue and Organ Culture, 33, 105 – 119.
Hutchinson, J.F., Beardsell, D.V. and Comb, J.A.M. (1985). Propagation by tissue culture introduction.
In Lamont, B.B. and Watkins, P.A. (eds). Horticulture of Australian Plants. Western Australian Dept. Agriculture, South Perth, W.A., 38 – 52.
Knudson, L. (1946). A new nutrient for the germination of orchid seeds. American Orchid Society Bulletin,
15, 214 – 217.
Kuljaroensub, V., Kunakhonnuruk, B. and Kongbangkerd, A. (2014). Effect of media and cytokinins on growth and development of in vitro culture of Panisea uniflora (Lindl.) Lindl. In Proceedings The 6th National Science Research Conference. (pp. 225 – 230). Burapha University. (in Thai)
Mary, S.S. and Divakar, K.M. (2015). In vitro propagation of epiphytic orchid Pholidota imbricata Hook. of Western Ghats. International Journal of Engineering Research & Technology, 4(10), 107 – 112.
Murashige, T. and Skoog, F. (1962). A revised medium for rapid growth and bioassay with tobacco tissue culture. Plant Physiology, 15, 473 – 497.
Nanakorn, W. and Indhamusika, S. (2000). Queen Sirikit Botanic Garden (Vol 6.) Chiang Mai: Wanida Press.
Nanakorn, W. and Watthana, S. (2008). Queen Sirikit Botanic Garden (Thai Native Orchids 2.) Chiang Mai: Wanida Press.
Pierik, R.L.M., Sprenkels, P.A., Harst, B.V.D. and Meys, Q.G.V.D. (1988). Seed germination and further development of plantlets of Paphiopedilum ciliolare Pfitz. in vitro. Scientia Horticulturae, 34, 139-153.
Razdan, M.K. (2002). Introduction to plant tissue culture. (2nd ed.) USA: Science Publishers.
Ritti, W., Thapyai, C. and Kongbangkerd, A. (2013). Effect of cytokinins and auxins on morphological changes of in vitro rhizome tip culture of Eulophia siamensis Rolfe ex Downie. In Proceedings of the 5th Science Research Conference. (pp. 207 – 213). University of Phayao. (in Thai)
Roy, A.R., Patel, R.S., Patel, V.V., Sajeev, S. and Deka, B.C. (2011). Asymbiotic seed germination, mass propagation and seedling development of Vanda coerulea Griff ex. Lindl. (Blue Vanda) an in vitro protocol for an endangered orchid. Scientia Horticulturae, 128, 325 – 331.
Ruengverayut, S., Bhinija, K., Loprasert, S. and Huehne, P.S. (2005). Effect of cytokinin on multiple shoots induction in Coelogyne, Dendrobium and Vanda orchids. In Proceedings of 43rd Kasetsart University Annual Conference: Plants. (pp. 467 – 474). Kasetsart University. (in Thai)
Santarunai, N. (2015). Effect of medium and plant growth regulator on development and flowering (Dendrobium friedericksianum Rchb.f.) of orchid in vitro. In The National Conference & Research Presentation 2015 “Create and Development to Approach ASEAN Community II”. (pp. 155 – 162). Nakhon Ratchasima College. (in Thai)
Shadang, R., Dwivedi, P., Hegde, S.H. and Ahmed, N. (2007). Effects of different culture media on seed germination and subsequent in vitro development of protocorms of Hygrochilus parishii (Veith & Rchb.f.) Pfitz (Orchidaceae). Indian Journal of Biotechnology, 6, 256 – 261.
Sittisujjatham, S. (2006). Wild Orchid of Thailand (6th ed). Bangkok: Baan Lae Suan Printing. (in Thai)
Stewart, S.L. and Kane, M.E. (2006). Asymbiotic seed germination and in vitro seedling development of Habenaria macroceratitis (Orchidaceae), a rare Florida terrestrial orchid. Plant Cell, Tissue and Organ Culture, 86, 147 – 158.
Street, H.E. (1977). Plant tissue and cell culture. (2nd ed) California: University of California Press.
Supinrach, S and Supinrach, I. (2014). Effects of BA and NAA on in vitro growth of Dendrobium lindleyi Steud. seedlings. Burapha Science Journal, 19(2), 84 – 92. (in Thai)
Taji, A.M. and Williams, R.R. (1996). Tissue culture of Australian plants. Australia: University of New England Press.
Thaithong, O. (2008). Thai orchids. (15th.) Bangkok: Baan Lae Suan Printing. (in Thai)
Trigiano, R.N. and Gray, D.J. (2004). Plant development and biotechnology. USA: CRC Press LLC.
Vacin, E. and Went, F. (1949). Some pH changes in nutrient solution. Botanical Gazette, 110, 605 – 613.
Vaz, A.P. and Kerbuay, G.B. (2000). Effects of mineral nutrients on in vitro growth and flower formation of Psygmorchis pusilla (Orchidaceae). Acta Horticulture, 250, 149 – 156.
Vejsadova, H. (2006). Factors affecting seed germination and seedling growth of terrestrial orchids cultured
in vitro. Acta Biologica Cracoviensia Series Botanica, 48(1), 109 – 113.
Vejsadova, H., Latalova, K., and Rizkova, R. (2002). Influence of growth regulators on terrestrial orchid culture under in vitro conditions. Acta Pruhoniciana, 73, 27 – 36.
Wattanawikkit, P., Bunn, E., Chayanarit, K. and Tantiwiwat, S. (2011). Effect of cytokinins (BAP and TDZ) and auxin (2,4-D) on growth and development of Paphiopedilum callosum. Kasetsart Journal Natural Science, 45, 12 – 19.
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2017-07-07
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