Effect of Light on Growth and Development of Protocorm and Seedling of Cymbidium seidenfadenii (P.J. Cribb & Du Puy) P.J. Cribb
Abstract
In vitro protocorms and seedlings of Cymbidium seidenfadenii (P.J. Cribb & Du Puy) P.J. Cribb) were cultured on Murashige and Skoog (MS) medium supplemented with 0.1 mgL-1 TDZ, and 1.0 mgL-1 NAA. The cultures were incubated under different light sources; cool white LED, warm white LED, cool white fluorescent, warm white fluorescent, blue, yellow, red, and green fluorescent light for 12 weeks. The results found that the blue light fluorescent could induce the highest number of PLBs (16.0protocorms) which was about 1.5-3.1 folds higher than other light sources, whereas the highest number of shoot buds (2.1shoot buds) could be noticed under the cool white fluorescent when using protocorms as explants. Seedlings explants were also incubated under different light treatments. The results showed that both blue light and cool white fluorescent could promote highest PLBs and shoot buds no statistically significant difference with non - significant difference.Keyword: Cymbidium seidenfadenii (P. J. Cribb & Du Puy) P. J. Cribb, Light, GrowthReferences
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Murashige, T. and Skoog, F. (1962). A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiologia Plantarum, 15(3), 473-497.
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Wang, Y., Li, Z., Huang, L. and Su, J. (2013). In vitro mass scale propagation of wild Cymbidium lowianum with a rare and endangered plant. American Journal of Plant Science, 4, 1500-1507.
Huan, L.V.T. and Tanaka, M. (2004). Effects of red and blue light-emitting diodes on callus induction, callus proliferation, and protocorm-like body formation from callus in Cymbidium orchid. Environmental Control in Biological, 42(1), 57-64.
Huché-Thélier, L., Crespel, L., Gourrierec, J., Morel, P., Sakr, S., and Leduc, N. (2016). Light signaling and plant responses to blue and UV radiations-Perspectives for applications in horticulture. Environmental and Experimental Botany, 121, 22-38.
Islam, T., Bhattacharjee, B., Islam, S.M.S. Uddain, J. and Subramanium, S. (2015). Axenic seed culture and in vitro mass propagation of Malayan wild orchid Cymbidium finlaysonianum Lindl. Pakistan Journal of Botany, 47(6), 2361-2367.
Kamal, M.M., Shimasaki, K. and Akter, N. (2014). Effect of light emitting diode (LED) lamps and N-acetylglucosamine (NAG) on organogenesis in protocorm-like bodies (PLBs) of a Cymbidium hybrid cultured in vitro. Plant Tissue Culture and Biotechnology, 24(2), 273-277.
Kami, C., Lorrain, S., Hornitschek, P., Fankhauser, C. (2010). Light-regulated plant growth and development. Current Topics in Developmental Biology, 91, 29-66.
Kannan, N. (2009). An in vitro study on micropropagation of Cymbidium orchids.Current Biotica, 3(2),
244-250.
Kaur, S. and Bhutani, K.K. (2012). Organic growth supplement stimulants for in vitro multiplication of Cymbidium pendulum (Roxb.) Sw. Horticultural Science, 39(1), 47-52.
Kim, S.J., Hahn, E.J., Heo, J.W., and Paek, K.Y. (2004). Effects of LEDs on net photosynthetic rate, growth and leaf stomata of chrysanthemum plantlets in vitro. Scientia Horticulturae, 101, 143-151.
Lin, C. (2000). Plant blue-light receptors.Trends in Plant Science, 5, 337-342.
Murashige, T. and Skoog, F. (1962). A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiologia Plantarum, 15(3), 473-497.
Nahar, S.J., Shimasaki, K. and Haque, S.M. (2012). Effect of polysaccharides including elicitors on organogenesis in protocorm-like body (PLB) of Cymbidium insigne in vitro. Journal of Agricultural Science and Technology, 2, 1029-1033.
Nahar, S.J., Haque, S.M. and Shimasaki, K. (2015). Organogenesis of Cymbidium finlaysonianum under different sources of lights.American-Eurasian Journal of Agricultural and Environmental Science, 15(10), 2095-2101.
Nhut, D.T., Takamura, T., and Watanabe, H. (2003). Responses of strawberry plantlets cultured in vitro under superbright red and blue light-emitting diodes (LEDs). Plant Cell Tissue and Organ Culture, 73,
43-52.
Wang, Y., Li, Z., Huang, L. and Su, J. (2013). In vitro mass scale propagation of wild Cymbidium lowianum with a rare and endangered plant. American Journal of Plant Science, 4, 1500-1507.
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2017-06-29
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บทความวิจัยจากการประชุมวิชาการระดับชาติ"วิทยาศาสตร์วิจัย"ครั้งที่ 9
