Stability of Dried Blood Spot Specimen for Thalassemia Diagnosis
Abstract
The dried blood spot (DBS) stability was studied for Hb typing and alpha-thalassemia 1 diagnosis. The specimens were investigated by Hb typing from normal Hb typing, Hb E trait and Homozygous Hb E; 9, 6 and 5 samples, respectively. Including, the alpha-thalassemia 1 (SEA and THAI deletion) was investigated for 20 samples compose of 16 negative and 4 positive for alpha-thalassemia 1 (SEA deletion). The DBS was kept at room temperature (RT), 37oC and 4oC after that the Hb typing and alpha-thalassemia 1 was investigated at 1, 7, 14, 30, 60 and 90 days. Hb typing results, the stability of Hb had the greatest at 4oC when compare to RT and 37oC for the same day. The Hb stability was decreased when prolong the storage time. Meanwhile alpha-thalassemia 1 results of DBS correlated with whole blood (100%) in all temperature and storage time of study. In this study, the sample collection as DBS has an advantage for thalassemia retesting. Keywords : Dried Blood Spot (DBS), Hb typing, alpha-thalassemia 1References
Fucharoen, G. (2009). Laboratory testing for Thalassemia diagnosis and Hemoglobinopathies in Thailand. Khon Kean: Khon Kean University Publisher. (in Thai)
Knudsen, R.C., Slazyk, W.E., Richmond, J.Y., Hannon, W.H. (1993). Guidelines from the Centers for Disease Control and Prevention for the shipment of dried blood spot specimens. Infant Screening, 16 (1), 1-3.
Luo, W., Yang, H., Rathbun, K., Pau, C., Ou, C. (2005). Detection of human immunodeficiency virus type 1 DNA in dried blood spots by a duplex real-time PCR assay. Journal of clinical microbiology, 43 (4), 1851-1857
Neonatal Screening Operation Centre. (2016). Retrieved February 21, 2016, from http://www.neoscreen.go.th
Pornprasert, S., Kasemrad, C., Sukunthamala, K. (2010). Diagnosis of thalassemia on dried blood spot samples by high performance liquid chromatography. Hemoglobin, 34 (5), 486-494.
The work manual committee of Thalassemia diagnosis and Hemoglobinopathies for laboratory. (2015) Revised Edition. Working manual, Thalassemia diagnosis and Hemoglobinopathies for laboratory. Bangkok: Knockout blow Publisher. (in Thai)
The work manual committee of Thalassemia diagnosis and Hemoglobinopathies for laboratory. (2016) Guideline for Thalassemia control and prevention for laboratory testing. Bangkok: Knockout blow Publisher. (in Thai)
Knudsen, R.C., Slazyk, W.E., Richmond, J.Y., Hannon, W.H. (1993). Guidelines from the Centers for Disease Control and Prevention for the shipment of dried blood spot specimens. Infant Screening, 16 (1), 1-3.
Luo, W., Yang, H., Rathbun, K., Pau, C., Ou, C. (2005). Detection of human immunodeficiency virus type 1 DNA in dried blood spots by a duplex real-time PCR assay. Journal of clinical microbiology, 43 (4), 1851-1857
Neonatal Screening Operation Centre. (2016). Retrieved February 21, 2016, from http://www.neoscreen.go.th
Pornprasert, S., Kasemrad, C., Sukunthamala, K. (2010). Diagnosis of thalassemia on dried blood spot samples by high performance liquid chromatography. Hemoglobin, 34 (5), 486-494.
The work manual committee of Thalassemia diagnosis and Hemoglobinopathies for laboratory. (2015) Revised Edition. Working manual, Thalassemia diagnosis and Hemoglobinopathies for laboratory. Bangkok: Knockout blow Publisher. (in Thai)
The work manual committee of Thalassemia diagnosis and Hemoglobinopathies for laboratory. (2016) Guideline for Thalassemia control and prevention for laboratory testing. Bangkok: Knockout blow Publisher. (in Thai)
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Published
2017-08-22
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บทความวิจัยจากการประชุมวิชาการระดับชาติ"วิทยาศาสตร์วิจัย"ครั้งที่ 9
