Production of Polyclonal Antibody Against Immunoglobulin of Tilapia Oreochromis niloticus
Abstract
Mouse antiserum against immunoglobulin (Ig) of tilapia (Oreochromis niloticus) was produced by using tilapia Ig/BSA complex. Tilapia anti-bovine serum albumin was produced first by peritoneal injection of bovine serum albumin (BSA) and then used for preparation of tilapia Ig/BSA complex by immunoprecipitation. The complex was then used for immunization into mice for production of mouse anti-tilapia Ig antiserum. The mouse anti-tilapia Ig antiserum demonstrated high affinity on both heavy chain (78 kDa) and light chain (23 kDa) as determined by Western blotting, however, the mouse antiserum still contained antibodies that slightly recognized BSA (60 kDa) and other components in tilapia serum as well. Therefore, further development of monoclonal antibody should be performed to obtain high specific antibody without limited quantity. Keywords : antiserum, Oreochromis niloticus, immunoglobulin, double immunodiffusion, Western blottingReferences
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Kitancharoen, N., Hanjavanij, C. & Suwannapeng, N. (2006). Efficiency of Vaccination with Streptococcus agalactiae bacterin on Streptococcosis Prevention in Nile tilapia. Khon Kaen University Research Journal, 11, 53-61. (in thai).
Kwon, S.R., Nam. Y.K., Kim. S.K. & Kim. K.H. (2006). Protection of tilapia (Oreochromis mosambicus) from edwardsiellosis by vaccination with Edwardsiella tarda ghost. Fish and Shellfish Immunology,
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Laemmli, UK. (1970). Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4. Nature, 227, 680-685.
Li, Q., Zhan, W., Xing J. & Sheng, X. (2007). Production, characterisation and applicability of monoclonal antibodies to immunoglobulin of Japanese flounder (Paralichthys olivaceus). Fish & Shellfish Immunology, 982-990.
Lim, S., Ho, H., Khader, S. M., & Kwang, J. (2009). First report on the isolation of immunoglobulin M of guppy, Poecilia reticulata, for the production of polyclonal antibodies. Aquaculture, 295 (1-2), 134-137.
Mian, G.F., Godoy, D.T., Leal, C.A.G., Yuhara, T.Y., Costa, G.M. & Figueiredo, H.C.P. (2008). Aspects of the natural history and virulence of Streptococcus agalactiae infection in Nile tilapia. Veterinary Microbiology, 136 (1–2), 180–183.
Mjoun, K. & Rosentrater, K. (2010). TILAPIA: Profile and Economic importance. South Dakota Cooperative Extension Service. Fact Sheets.
Rathore, G., Kumar, G., Sood, N., Kapoor, D. & Lakra, W.S. (2008). Development of monoclonal antibodies to rohu [Labeo rohita] immunoglobulins for use in immunoassays. Fish & Shellfish Immunology,
761-774.
Santos, M.D., Saito-Taki, T., Takano, T., Kondo, H., Hirono, I. & Aoki, T. (2009). Characterization of polyclonal antibodies against Japanese flounder IgM derived from recombinant IgM constant region proteins. Fish & Shellfish Immunology, 374–378.
Smith, S.A., Gebhard, D.H., Housman, J.M., Levy, M.G. & Noga, E.J. (1993). Isolation, purification, and molecular-weight determination of serum immunoglobulin from Oreochromis aureus. Journal of Aquatic Animal Health, 5, 23–35.
Vendrell, D., Balcázar, J.L., Ruiz-Zarzuela, I., de Blas, I., Gironés, O. & Múzquiz. J.L. (2006). Lactococcus garvieae in fish : A review. Comparative Immunology, Microbiology and Infectious Diseases, 29 (4), 177–198.
Vesely, T., Reschova, S., Pokorova, D., Hulova, J. & Nevorankova, Z. (2006). Production of monoclonal antibodies against immunoglobulin heavy chain in common carp (Cyprinus carpio L.). Veterinarni Medicina, 51 (5), 296–302.
Bullock, G. L. & Herman, R.L. (1985). "EDWARDSIELLA INFECTIONS OF FISHES”. US Fish & Wildlife Publications, 1-6.
Department of Fisheries. (2016a). Nile tilapia. Retrieved April 1, 2016, from http://www.fisheries.go.th/ if-phayao/ cultivate/c_nile.htm. (in thai)
Department of Fisheries. (2016b). Biology of Nile tilapia. Retrieved April 1, 2016, from http://www. fisheries.go.th/sf-mukdahan/web2/%5Cimages/planine/chwa.pdf. (in thai)
Kitancharoen, N., Hanjavanij, C. & Suwannapeng, N. (2006). Efficiency of Vaccination with Streptococcus agalactiae bacterin on Streptococcosis Prevention in Nile tilapia. Khon Kaen University Research Journal, 11, 53-61. (in thai).
Kwon, S.R., Nam. Y.K., Kim. S.K. & Kim. K.H. (2006). Protection of tilapia (Oreochromis mosambicus) from edwardsiellosis by vaccination with Edwardsiella tarda ghost. Fish and Shellfish Immunology,
621-626.
Laemmli, UK. (1970). Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4. Nature, 227, 680-685.
Li, Q., Zhan, W., Xing J. & Sheng, X. (2007). Production, characterisation and applicability of monoclonal antibodies to immunoglobulin of Japanese flounder (Paralichthys olivaceus). Fish & Shellfish Immunology, 982-990.
Lim, S., Ho, H., Khader, S. M., & Kwang, J. (2009). First report on the isolation of immunoglobulin M of guppy, Poecilia reticulata, for the production of polyclonal antibodies. Aquaculture, 295 (1-2), 134-137.
Mian, G.F., Godoy, D.T., Leal, C.A.G., Yuhara, T.Y., Costa, G.M. & Figueiredo, H.C.P. (2008). Aspects of the natural history and virulence of Streptococcus agalactiae infection in Nile tilapia. Veterinary Microbiology, 136 (1–2), 180–183.
Mjoun, K. & Rosentrater, K. (2010). TILAPIA: Profile and Economic importance. South Dakota Cooperative Extension Service. Fact Sheets.
Rathore, G., Kumar, G., Sood, N., Kapoor, D. & Lakra, W.S. (2008). Development of monoclonal antibodies to rohu [Labeo rohita] immunoglobulins for use in immunoassays. Fish & Shellfish Immunology,
761-774.
Santos, M.D., Saito-Taki, T., Takano, T., Kondo, H., Hirono, I. & Aoki, T. (2009). Characterization of polyclonal antibodies against Japanese flounder IgM derived from recombinant IgM constant region proteins. Fish & Shellfish Immunology, 374–378.
Smith, S.A., Gebhard, D.H., Housman, J.M., Levy, M.G. & Noga, E.J. (1993). Isolation, purification, and molecular-weight determination of serum immunoglobulin from Oreochromis aureus. Journal of Aquatic Animal Health, 5, 23–35.
Vendrell, D., Balcázar, J.L., Ruiz-Zarzuela, I., de Blas, I., Gironés, O. & Múzquiz. J.L. (2006). Lactococcus garvieae in fish : A review. Comparative Immunology, Microbiology and Infectious Diseases, 29 (4), 177–198.
Vesely, T., Reschova, S., Pokorova, D., Hulova, J. & Nevorankova, Z. (2006). Production of monoclonal antibodies against immunoglobulin heavy chain in common carp (Cyprinus carpio L.). Veterinarni Medicina, 51 (5), 296–302.
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2017-12-01
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