Effect of Allelopathic from Synedrella nodiflora (L.) Gaertn. Leaves on Seed Germination, Growth, and Physiology of Rice (Oryza sativa L.)
Abstract
The effect of 95% ethanolic leaf-extract from Synedrella nodiflora (L.) Gaertn. on germination and growth of rice plants were examined. The results demonstrated the inhibition effect of extract to the germination of rice. The higher extract concentration showed the higher seed-germination inhibition. The inhibition concentration at 50% seed germination (IC50) was determined and IC50 was 15.16 g/L. The extract at the IC50 was used to determine the osmotic potential which was -0.19 MPa. After that the effect of osmotic potential on the germination and growth of rice was determined by comparing to sucrose, sodium chloride and potassium nitrate at the same ΨS as the extract. The result showed that sucrose, sodium chloride and potassium nitrate did not inhibit seed germination and root growth while the extract showed the inhibition effect. This revealed that the osmotic potential at -0.19 MPa did not effect on rice seed germination and root growth. Water absorption of seeds was also determined and showed that extract did not inhibit water absorption. The reducing sugar and starch content in 7 days treated seeds were examined. Extract treated seeds showed lower reducing sugar and higher starch than in control. The activity of α-amylase was studied and showed lower activity in extract treatment than in control. Keywords : allelopathy, Synedrella nodiflora (L.) Gaertn., germination physiology, Oryza sativa L., α-amylaseReferences
Aslani, F., Juraimi, A. S., Ahmad-Hamdani, M. S., Hashemi, F. S. G., Alam, M. A., Hakim, M. A., & Uddin, M. K. (2016). Effects of Tinospora tuberculata leaf methanol extract on seedling growth of rice and associated weed species in hydroponic culture. Journal of Integrative Agriculture, 15(7), 1521-1531.
Babula, P., Vaverkova, V., Poborilova, Z., Ballova, L., Masarik, M., & Provaznik, I. (2014). Phytotoxic action of naphthoquinone juglone demonstrated on lettuce seedling roots. Plant Physiology and Biochemistry, 84, 78-86.
Batish, D. R., Setia, N., Singh, H. P., & Kohli, R. K. (2004). Phytotoxicity of lemon-scented eucalypt oil and its potential use as a bioherbicide. Crop Protection, 23(12), 1209-1214.
Bewley, J. D. (2001). Seed germination and reserve mobilization. Reviews in the Life Sciences, 1-7.
Bich, T.T.N. & Kato-Noguchi, H. (2012). Allelopathic potential of two aquatic plants, duckweed (Lemna minor L.) and water lettuce (Pistia stratiotes L.), on terrestrial plant species. Aquatic Botany, 103, 30-36.
Bisio, A., Fraternale, D., Giacomini, M., Giacomelli, E., Pivetti, S., Russo, Caviglioli, G., Romussi, G., Ricci, D., & De Tommasi, N. (2010). Phytotoxicity of Salvia spp. exudates. Crop Protection, 29(12), 1434-1446.
Changsawake, K., Laosinwattana, C., & Teerarak, M. (2010). Inhibitory potential of crude extract from Acacia pennata (L.) willd. subsp. insuavis Nielsen on germination, seeding growth and cell division of bioassay plants. King Mongkuts Agricultural Journal, 28(2), 65-73. (in Thai)
Chatiyanon, B. (2001). Inhibitory effect of Aglaia odorata Lour. leaf extract on germination and growth of some plants. Master’s thesis, Horticulture, Faculty of Agricultural, King Mongkut’s Institute of Technology Ladkrabang. (in Thai)
Dayan, F. E., Cantrell, C. L., & Duke, S. O. (2009). Natural products in crop protection. Bioorganic & Medicinal Chemistry, 17(12), 4022-4034.
Djurdjevic, L., Dinic, A., Pavlovic, P., Mitrovic, M., Karadzic, B., & Tesevic, V. (2004). Allelopathic potential of Allium ursinum L. Biochemical Systematics and Ecology, 32(6), 533-544.
Finney, D. J. (1971). Probit Analysis. Cambridge: Cambridge University Press.
Ghayal, NA., Biware, MV., & Dhumal, KN. (2014). Effect of leachates of alien weeds on seed germination, seedling growth and physiology in mungbean. International Journal of Agriculture and Biosciences, 3(4), 141–148.
Jala, A., & Wongsarasin, A. (2013). Effect of allelopathy from Ruellia tuberosa on germination rate of weed seeds (Mimosa pucida L., Amaranthus gracilis Desf. and Cleoma viscosa L.). Journal of Science and Technology is a publication of Thammasat University, 21(6), 558-564. (in Thai)
Jelassi, A., Ayeb-Zakhama, A. El, Nejma, A. Ben, Chaari, A., Harzallah-Skhiri, F., & Jannet, H. Ben. (2016). Phytochemical composition and allelopathic potential of three Tunisian Acacia species. Industrial Crops and Products, 83, 339-345.
Kato-Noguchi, H., & Macías, F. A. (2005). Effects of 6-methoxy-2-benzoxazolinone on the germination and α-amylase activity in lettuce seeds. Journal of Plant Physiology, 162(12), 1304-1307.
Khamriang, S., Sinsiri, W., Sinsiri, N., & Kaewduangta, W. (2014). Efficacy of crude extract of Alpinia galangal (Linn.) Swartz on germination and growth of some crops and weeds. Khon Kaen Agriculutre Journal, 42(1), 57-62. (in Thai)
Kil, J. H., Shim, K. C., Lee, K. J., (2002). Allelopathy of Tagetes minuta L. aqueous extracts on seed germination and root hair growth. Korean Journal Ecology Science, 1, 171-174.
Lertmongkol, S. (2014). Allelopathic effect of fresh and dry Cleome rutidosperma DC. extracts on seed germination and growth of Echinochloa crus-galli Beauv. and Amaranthus spinosus L. Thai Agricultural Research Journal, 32(1), 68-76. (in Thai)
Ma, J., Xing, G., Yang, W., Ma, L., Gao, M., Wang, Y., & Han, Y. (2012). Inhibitory effects of leachate from Eupatorium adenophorum on germination and growth of Amaranthus retroflexus and Chenopodium glaucum. Acta Ecologica Sinica, 32(1), 50-56.
Madany, M. M. Y., & Saleh, A. M. (2015). Phytotoxicity of Euphorbia helioscopia L. on Triticum aestivum L. and Pisum sativum L. Annals of Agricultural Sciences, 60(1), 141-151.
Murataza, G., Asghara, A. R. (2012). α-Amylase activityes during seed development and germination in pea (Pisum sativum L.) treated with salicylic acid. Pakistan Journal of Botany, 44(6), 1823-1829.
Morris, P. J., & Parrish, D. J. (1992). Effects of sunflower residues and tillage on winter wheat. Field Crops Research, 29(4), 317-327.
Palacios, S. M., Corral del, S., Carpinella, M. C., & Ruiz, G. (2010). Screening for natural inhibitors of germination and seedling growth in native plants from Central Argentina. Industrial Crops and Products, 32, 674-677.
Patsai, S. (2011). Allelophathic effect of Praxelis clematidea (Griseb.) R.M. King & H. Rob on germination and growth of some crops. Master of Education Degree in Biology, Srinakharinwirot University. (in Thai)
Perata, P., Guglielminetti, L., & Alpi, A. (1997). Mobilization of endosperm reserves in cereal seeds under anoxia. Annals of Botany, 79, 49-56.
Phraprasert, P., & Namnamung, W. (2005). Effect of crude extracts from Synedrella nodiflora (L.) Gaertn. By various solvents on seed germination and growth of some plants. Burapha Science Journal, 10(1-2), 68-75. (in Thai)
Poonpaiboonpipat, T., Pangnakorn, U., Suvunnamek, U., Teerarak, M., Charoenying, P., & Laosinwattana, C. (2013). Phytotoxic effects of essential oil from Cymbopogon citratus and its physiological mechanisms on barnyardgrass (Echinochloa crus-galli). Industrial Crops and Products, 41, 403-407.
Rattanapanone, N. (2006). Food chemistry (2 nd ed.). Bangkok: Odeon Store. (in Thai)
Rawat, L. S., Maikhuri, R. K., & Negi, V. S. (2013). Inhibitory effect of leachate from Helianthus annuus on germination and growth of Kharif crops and weeds. Acta Ecologica Sinica, 33(5), 245-252.
Rietveld, W. J. (1983). Allelopathic effects of juglone on germination and growth of several herbaceous and woody species. Journal of Chemical Ecology, 9(2), 295-308.
Rose, R., Rose, C. L., Omi, S. K., Foory. K.R., Durall, D. M. & Bigg, W. L. (1991). Starch determination by perchloric acid vs enzymes: evaluation the accuracy and precistion of six coloricmetric methods. Journal of Agricultural and Food Chemistry, 39, 2-11.
Scrivanti, L. R. (2010). Allelopathic potential of Bothriochloa laguroides var. laguroides (DC.) Herter (Poaceae: Andropogoneae). Flora: Morphology, Distribution, Functional Ecology of Plants, 205(5), 302-305.
Silva, R. M. G., Brigatti, J. G. F., Santos, V. H. M., Mecina, G. F., & Silva, L. P. (2013). Allelopathic effect of the peel of coffee fruit. Scientia Horticulturae, 158, 39-44.
Techapinyawat, S. (2005). Plant physiology (4 th ed.). Bangkok: Kasetsart University Press. (in Thai)
Turk, M. A., & Tawaha, A. M., (2003). Allelopathic effect of black mustard (Brassica nigra L.) on germination and growth of wild oat (Avena fatua L.). Crop Protection, 22, 673-677.
Uddin, M., Robinson, R., Buultjens, A., Al Harun, M., & Shampa, S. (2017). Role of allelopathy of Phragmites australis in its invasion processes. Journal of Experimental Marine Biology and Ecology. 486, 237-244.
William, C. S. & F. William S. (1931). A method for the determination of the freezing point depression of aqueous solutions particularly those containing protein. Journal of Biological Chemistry, 91, 217-226.
Yenish, J. P., Worsham, A. D., & Chilton, W. S. (1995). Disappearance of DIBOA-glucoside, DIBOA, and BOA from rye (Secale cereal L.) cover crop residue. Weed Science, 43, 18-20.
Zungsontiporn, S., & Premasthira, C. (1994). Effect of crude extract of Eupatorium adenophorum Spreng. on germination and growth of some crops and weeds. Thai Agricultural Research Journal, 12(1), 37-41. (in Thai)
Babula, P., Vaverkova, V., Poborilova, Z., Ballova, L., Masarik, M., & Provaznik, I. (2014). Phytotoxic action of naphthoquinone juglone demonstrated on lettuce seedling roots. Plant Physiology and Biochemistry, 84, 78-86.
Batish, D. R., Setia, N., Singh, H. P., & Kohli, R. K. (2004). Phytotoxicity of lemon-scented eucalypt oil and its potential use as a bioherbicide. Crop Protection, 23(12), 1209-1214.
Bewley, J. D. (2001). Seed germination and reserve mobilization. Reviews in the Life Sciences, 1-7.
Bich, T.T.N. & Kato-Noguchi, H. (2012). Allelopathic potential of two aquatic plants, duckweed (Lemna minor L.) and water lettuce (Pistia stratiotes L.), on terrestrial plant species. Aquatic Botany, 103, 30-36.
Bisio, A., Fraternale, D., Giacomini, M., Giacomelli, E., Pivetti, S., Russo, Caviglioli, G., Romussi, G., Ricci, D., & De Tommasi, N. (2010). Phytotoxicity of Salvia spp. exudates. Crop Protection, 29(12), 1434-1446.
Changsawake, K., Laosinwattana, C., & Teerarak, M. (2010). Inhibitory potential of crude extract from Acacia pennata (L.) willd. subsp. insuavis Nielsen on germination, seeding growth and cell division of bioassay plants. King Mongkuts Agricultural Journal, 28(2), 65-73. (in Thai)
Chatiyanon, B. (2001). Inhibitory effect of Aglaia odorata Lour. leaf extract on germination and growth of some plants. Master’s thesis, Horticulture, Faculty of Agricultural, King Mongkut’s Institute of Technology Ladkrabang. (in Thai)
Dayan, F. E., Cantrell, C. L., & Duke, S. O. (2009). Natural products in crop protection. Bioorganic & Medicinal Chemistry, 17(12), 4022-4034.
Djurdjevic, L., Dinic, A., Pavlovic, P., Mitrovic, M., Karadzic, B., & Tesevic, V. (2004). Allelopathic potential of Allium ursinum L. Biochemical Systematics and Ecology, 32(6), 533-544.
Finney, D. J. (1971). Probit Analysis. Cambridge: Cambridge University Press.
Ghayal, NA., Biware, MV., & Dhumal, KN. (2014). Effect of leachates of alien weeds on seed germination, seedling growth and physiology in mungbean. International Journal of Agriculture and Biosciences, 3(4), 141–148.
Jala, A., & Wongsarasin, A. (2013). Effect of allelopathy from Ruellia tuberosa on germination rate of weed seeds (Mimosa pucida L., Amaranthus gracilis Desf. and Cleoma viscosa L.). Journal of Science and Technology is a publication of Thammasat University, 21(6), 558-564. (in Thai)
Jelassi, A., Ayeb-Zakhama, A. El, Nejma, A. Ben, Chaari, A., Harzallah-Skhiri, F., & Jannet, H. Ben. (2016). Phytochemical composition and allelopathic potential of three Tunisian Acacia species. Industrial Crops and Products, 83, 339-345.
Kato-Noguchi, H., & Macías, F. A. (2005). Effects of 6-methoxy-2-benzoxazolinone on the germination and α-amylase activity in lettuce seeds. Journal of Plant Physiology, 162(12), 1304-1307.
Khamriang, S., Sinsiri, W., Sinsiri, N., & Kaewduangta, W. (2014). Efficacy of crude extract of Alpinia galangal (Linn.) Swartz on germination and growth of some crops and weeds. Khon Kaen Agriculutre Journal, 42(1), 57-62. (in Thai)
Kil, J. H., Shim, K. C., Lee, K. J., (2002). Allelopathy of Tagetes minuta L. aqueous extracts on seed germination and root hair growth. Korean Journal Ecology Science, 1, 171-174.
Lertmongkol, S. (2014). Allelopathic effect of fresh and dry Cleome rutidosperma DC. extracts on seed germination and growth of Echinochloa crus-galli Beauv. and Amaranthus spinosus L. Thai Agricultural Research Journal, 32(1), 68-76. (in Thai)
Ma, J., Xing, G., Yang, W., Ma, L., Gao, M., Wang, Y., & Han, Y. (2012). Inhibitory effects of leachate from Eupatorium adenophorum on germination and growth of Amaranthus retroflexus and Chenopodium glaucum. Acta Ecologica Sinica, 32(1), 50-56.
Madany, M. M. Y., & Saleh, A. M. (2015). Phytotoxicity of Euphorbia helioscopia L. on Triticum aestivum L. and Pisum sativum L. Annals of Agricultural Sciences, 60(1), 141-151.
Murataza, G., Asghara, A. R. (2012). α-Amylase activityes during seed development and germination in pea (Pisum sativum L.) treated with salicylic acid. Pakistan Journal of Botany, 44(6), 1823-1829.
Morris, P. J., & Parrish, D. J. (1992). Effects of sunflower residues and tillage on winter wheat. Field Crops Research, 29(4), 317-327.
Palacios, S. M., Corral del, S., Carpinella, M. C., & Ruiz, G. (2010). Screening for natural inhibitors of germination and seedling growth in native plants from Central Argentina. Industrial Crops and Products, 32, 674-677.
Patsai, S. (2011). Allelophathic effect of Praxelis clematidea (Griseb.) R.M. King & H. Rob on germination and growth of some crops. Master of Education Degree in Biology, Srinakharinwirot University. (in Thai)
Perata, P., Guglielminetti, L., & Alpi, A. (1997). Mobilization of endosperm reserves in cereal seeds under anoxia. Annals of Botany, 79, 49-56.
Phraprasert, P., & Namnamung, W. (2005). Effect of crude extracts from Synedrella nodiflora (L.) Gaertn. By various solvents on seed germination and growth of some plants. Burapha Science Journal, 10(1-2), 68-75. (in Thai)
Poonpaiboonpipat, T., Pangnakorn, U., Suvunnamek, U., Teerarak, M., Charoenying, P., & Laosinwattana, C. (2013). Phytotoxic effects of essential oil from Cymbopogon citratus and its physiological mechanisms on barnyardgrass (Echinochloa crus-galli). Industrial Crops and Products, 41, 403-407.
Rattanapanone, N. (2006). Food chemistry (2 nd ed.). Bangkok: Odeon Store. (in Thai)
Rawat, L. S., Maikhuri, R. K., & Negi, V. S. (2013). Inhibitory effect of leachate from Helianthus annuus on germination and growth of Kharif crops and weeds. Acta Ecologica Sinica, 33(5), 245-252.
Rietveld, W. J. (1983). Allelopathic effects of juglone on germination and growth of several herbaceous and woody species. Journal of Chemical Ecology, 9(2), 295-308.
Rose, R., Rose, C. L., Omi, S. K., Foory. K.R., Durall, D. M. & Bigg, W. L. (1991). Starch determination by perchloric acid vs enzymes: evaluation the accuracy and precistion of six coloricmetric methods. Journal of Agricultural and Food Chemistry, 39, 2-11.
Scrivanti, L. R. (2010). Allelopathic potential of Bothriochloa laguroides var. laguroides (DC.) Herter (Poaceae: Andropogoneae). Flora: Morphology, Distribution, Functional Ecology of Plants, 205(5), 302-305.
Silva, R. M. G., Brigatti, J. G. F., Santos, V. H. M., Mecina, G. F., & Silva, L. P. (2013). Allelopathic effect of the peel of coffee fruit. Scientia Horticulturae, 158, 39-44.
Techapinyawat, S. (2005). Plant physiology (4 th ed.). Bangkok: Kasetsart University Press. (in Thai)
Turk, M. A., & Tawaha, A. M., (2003). Allelopathic effect of black mustard (Brassica nigra L.) on germination and growth of wild oat (Avena fatua L.). Crop Protection, 22, 673-677.
Uddin, M., Robinson, R., Buultjens, A., Al Harun, M., & Shampa, S. (2017). Role of allelopathy of Phragmites australis in its invasion processes. Journal of Experimental Marine Biology and Ecology. 486, 237-244.
William, C. S. & F. William S. (1931). A method for the determination of the freezing point depression of aqueous solutions particularly those containing protein. Journal of Biological Chemistry, 91, 217-226.
Yenish, J. P., Worsham, A. D., & Chilton, W. S. (1995). Disappearance of DIBOA-glucoside, DIBOA, and BOA from rye (Secale cereal L.) cover crop residue. Weed Science, 43, 18-20.
Zungsontiporn, S., & Premasthira, C. (1994). Effect of crude extract of Eupatorium adenophorum Spreng. on germination and growth of some crops and weeds. Thai Agricultural Research Journal, 12(1), 37-41. (in Thai)
Downloads
Published
2017-09-15
Issue
Section
Research Article
