In vitro Plant Regeneration via Callus Culture in Turmeric (Curcuma longa L.)
Abstract
Buds of rhizome and sprout of Curcuma longa L. which collected from Bantakun and Thachana district were cultured under aseptic condition to induce callus formation. The MS culture media containing various concentrations of 2, 4-D or NAA or BAP were used. The results showed that various types of callus were achieved depending upon types and plant growth regulator ratios in the culture media. Only the pale brown callus was capable subculture to extend the number of callus masses. When the callus transferred to modified MS regeneration media, numerous green nodes occurred and subsequently developed to shoots. Maximum plant regeneration was achieved on the medium supplemented with 0.1 mg/l NAA + 3.0 mg/l BAP (15.8 plants, turmeric collected from Bantakhun and 11.9 plants, turmeric collected from Thachana, respectively). Prolific roots of shoots were obtained when the shoots were transferred to MS culture medium containing 1.0 or 3.0 mg/l NAA. High survival of plantlets was attained after acclimatization in potted-soils covering with transparent plastic bags. Morphological observations of callus derived plantlets did not differ to maternal plant.Keywords: callus, plant regeneration, turmericReferences
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Anjaneyulu, E., Hemalatha, S., Bharath Raj, S.,&Balaji, M. (2011).Callus induction and plant regeneration in finger Millet (Eleusinecoracana L.). Libyan Agriculture Research Center Journal International, 2, 57-61.
Bausher, M.G.,&Yolenosky, G. (1987). Morphological changes in Citrus association with relatively high concentration of paclobutrazol. Journal of Plant Growth Regulation, 5, 139-147.
Borjian, L.,& Arak, H. (2013). A study on the effect of different concentration of plant hormones (BAP, NAA, Kinetin, and 2, 4-D) on callus induction in Brassica napus.International Research Journal of Applied and Basic Sciences, 5, 519-521.
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Ghosh, A., Chatterjee, P.,&Ghosh, P. (2013).A protocol for rapid propagation of genetically true to type Indian Turmeric (Curcuma longa L.) through in vitro culture technique.Advances in Applied Science Research, 4, 39-45.
Hegde, M.N., Shetty, S., Yelapure, M.,&Patil, A. (2012). Evaluation of antimicrobial activity of aqueous and hydro-alcoholic Curcuma Longaextracts against endodontic pathogens. IOSR Journal of Pharmacy, 2,192-198.
Misra, S.K.,&Sahu, K.C. (1977).Screening of some indigenous plants for antifungal activity against dermatophytes.Indian Journal of Pharmacology,9, 269-272.
Mukhri, T.,& Yamaguchi, H. (1986).In vitroplant multiplication from rhizomes of Turmeric (Curcuma domestica Val.) and ternoelawak (C. xanthorhizaRoxb.).Plant Tissue Culture Letter, 3, 28-30.
Murashige, T.,&Skoog, F. (1962).A revised medium for rapid growth and bio-assays with Tobacco tissue cultures.PhysiologiaPlantarum, 15, 473-497.
Nkere, C.K.,&Mbanaso, E.N.A. (2010).Optimizing concentrations of growth regulators for in-vitro Ginger propagation.Journal of Agrobiology, 27, 61–65.
Nasirujjaman, K., Uddin, M.S., Zaman, S.,& Reza, M.A. (2005).Micropropagation of Turmeric (Curcuma longa Linn.) through in vitro rhizome bud culture.Journal of Biological Science, 5, 490-492.
Naz, S., Ilyas, S., Javad, S.,& Ali, A. (2009). In vitroclonal multiplication and acclimatization of different varieties of Turmeric (Curcuma longa L.).Pakistan Journal of Botany, 41, 2807-2816.
Palve, Y.P.,&Nayak, P.L. (2012).Curcumin: A wonder anticancer drug.IJPRBS, 3, 60-69.
Ramesh, M., Murugiah, V.,& Gupta, A.K. (2009). Efficient in vitroplant regeneration vialeaf base segments of indicaRice (Oryza sativa L.). Indian Journal of Experimental Biology, 47, 68-74.
Roopadarshini, V. (2010). High frequency shoots multiplication and callus regeneration of Turmeric. International Journal of Biochemical Biotechnology, 6, 723–733.
Saensouk, P. (2011). Callus induction and plant regeneration from leaf explant of Cornukaempferiaaurantiflora Mood & Larsen. Pakistan Journal of Botany, 43, 2415-2418.
Saensouk, P., Theerakulpisut, P., Kijwijan, B.,&Bunnag, S. (2007). Effects of 2,4-D on callus induction from leaf explants of CornukaempferialarseniiP. Saensouk. Gardens’ Bulletin Singapore, 59, 183-188.
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Shrishail, D., Handralharish, K., Ravichandra, H., Tulsianand, G.,&Shruthi, S.D. (2013). Turmeric: Nature’s precious medicine. Asian Journal Pharmaceutical and Clinical Research, 6, 10-16.
Srangsam, A.,&Kanchanapoom, K. (2003).Thidiazuron induced plant regeneration in callus culture of triploid Banana (Musa sp.) ‘Gros Michel’, AAA group. Songklanakarin Journal of Science and Technology, 25, 689-696.
Srangsam, A.,&Kanchanapoom, K. (2007). Establishment of in vitroculture of Musa AA group ‘KluaiSa’ and Musa AA group ‘KluaiLebMue Nang’ and the analysis of ploidystability. ScienceAsia, 33, 437-442.
Surh, Y.J. (2002). Anti-tumor promoting potential of selected spice in- gradients with anti-oxidative and anti-
inflammatoryactivities: a short review. Food Chemical Toxicology, 40, 1091–1097.
Taha H.S., Abbas, M.S., Aly, U.I.,&Gaber, I. (2013).New aspects for callus production, regeneration and molecularcharacterization of Ginger (ZingiberofficinaleRosc.)Medicinal & Aromatic Plants, 6, 2-8.
Taiz, L.,& Zeiger, E. (2002). Mineral nutrition: Plant physiology. 2nd ed. Sinaver Associates Inc. Pub. p. 67-86.
Te-chato, S., Hilae, A.,&Yedum, I. (2002).Improve callus induction and embryogeniccallus formation from cultured young leaves of Oil palm seedling. Thai Journal of Agricultural Science, 35, 407–13.
Tsay, H.,& Tseng, M. (1979).Embryoidformation and plantlet regeneration from anther callus of sweet Potato. Botanical Bulletin AcademiaSinica, 20, 117-122.
Wernicke, W., & Brettell, R. (1980). Somatic embryogenesis from Sorghum bicolorleaf.Nature, 287, 138 -139.
Yam, T.W.,&Arditti, J. (2009). History of orchid propagation: a mirror of the history of biotechnology.
Plant Biotechnology Report, 3, 1-56.
Zapata, E.V., Morales, G.S., Lauzardo, A.N.H., Bonfil, B.M., Tapia, G.T., Sanchez, A.D.J., Valle, M.V.D.,&Aparicio A.J. (2003).In vitroregeneration and acclimatization of plants of Turmeric (Curcuma longa L.)
in a hydroponic system.Biotechnology Applied Biochemistry, 20, 25-31.
Anis, M., Faisal, M.,& Singh, S.K. (2003).Micropropagation of Mulberry (Morusalba L.) through in vitroculture of shoot tip and nodal explants. Plant Tissue Culture, 13, 47-51.
Anjaneyulu, E., Hemalatha, S., Bharath Raj, S.,&Balaji, M. (2011).Callus induction and plant regeneration in finger Millet (Eleusinecoracana L.). Libyan Agriculture Research Center Journal International, 2, 57-61.
Bausher, M.G.,&Yolenosky, G. (1987). Morphological changes in Citrus association with relatively high concentration of paclobutrazol. Journal of Plant Growth Regulation, 5, 139-147.
Borjian, L.,& Arak, H. (2013). A study on the effect of different concentration of plant hormones (BAP, NAA, Kinetin, and 2, 4-D) on callus induction in Brassica napus.International Research Journal of Applied and Basic Sciences, 5, 519-521.
Eapen, S.,& George, L. (1990). Influence ofphytohormones, carbohydrates, amino acids, growth supplements and antibiotics on somatic embryogenesis and plant differentiation in finger Millet. Plant Cell Tissue and Organ Culture, 22, 87-93.
Ghosh, A., Chatterjee, P.,&Ghosh, P. (2013).A protocol for rapid propagation of genetically true to type Indian Turmeric (Curcuma longa L.) through in vitro culture technique.Advances in Applied Science Research, 4, 39-45.
Hegde, M.N., Shetty, S., Yelapure, M.,&Patil, A. (2012). Evaluation of antimicrobial activity of aqueous and hydro-alcoholic Curcuma Longaextracts against endodontic pathogens. IOSR Journal of Pharmacy, 2,192-198.
Misra, S.K.,&Sahu, K.C. (1977).Screening of some indigenous plants for antifungal activity against dermatophytes.Indian Journal of Pharmacology,9, 269-272.
Mukhri, T.,& Yamaguchi, H. (1986).In vitroplant multiplication from rhizomes of Turmeric (Curcuma domestica Val.) and ternoelawak (C. xanthorhizaRoxb.).Plant Tissue Culture Letter, 3, 28-30.
Murashige, T.,&Skoog, F. (1962).A revised medium for rapid growth and bio-assays with Tobacco tissue cultures.PhysiologiaPlantarum, 15, 473-497.
Nkere, C.K.,&Mbanaso, E.N.A. (2010).Optimizing concentrations of growth regulators for in-vitro Ginger propagation.Journal of Agrobiology, 27, 61–65.
Nasirujjaman, K., Uddin, M.S., Zaman, S.,& Reza, M.A. (2005).Micropropagation of Turmeric (Curcuma longa Linn.) through in vitro rhizome bud culture.Journal of Biological Science, 5, 490-492.
Naz, S., Ilyas, S., Javad, S.,& Ali, A. (2009). In vitroclonal multiplication and acclimatization of different varieties of Turmeric (Curcuma longa L.).Pakistan Journal of Botany, 41, 2807-2816.
Palve, Y.P.,&Nayak, P.L. (2012).Curcumin: A wonder anticancer drug.IJPRBS, 3, 60-69.
Ramesh, M., Murugiah, V.,& Gupta, A.K. (2009). Efficient in vitroplant regeneration vialeaf base segments of indicaRice (Oryza sativa L.). Indian Journal of Experimental Biology, 47, 68-74.
Roopadarshini, V. (2010). High frequency shoots multiplication and callus regeneration of Turmeric. International Journal of Biochemical Biotechnology, 6, 723–733.
Saensouk, P. (2011). Callus induction and plant regeneration from leaf explant of Cornukaempferiaaurantiflora Mood & Larsen. Pakistan Journal of Botany, 43, 2415-2418.
Saensouk, P., Theerakulpisut, P., Kijwijan, B.,&Bunnag, S. (2007). Effects of 2,4-D on callus induction from leaf explants of CornukaempferialarseniiP. Saensouk. Gardens’ Bulletin Singapore, 59, 183-188.
Singh, D., Singh, H., Nongalleima, K., Moirangthem, S.,& Devi, S. (2013). Analysis of growth, yield potential and horticultural performance of conventional vs. micropropagatedplants of Curcuma longa var. Lakadong.
African Journal of Biotechnology, 12, 1604-1608.
Shrishail, D., Handralharish, K., Ravichandra, H., Tulsianand, G.,&Shruthi, S.D. (2013). Turmeric: Nature’s precious medicine. Asian Journal Pharmaceutical and Clinical Research, 6, 10-16.
Srangsam, A.,&Kanchanapoom, K. (2003).Thidiazuron induced plant regeneration in callus culture of triploid Banana (Musa sp.) ‘Gros Michel’, AAA group. Songklanakarin Journal of Science and Technology, 25, 689-696.
Srangsam, A.,&Kanchanapoom, K. (2007). Establishment of in vitroculture of Musa AA group ‘KluaiSa’ and Musa AA group ‘KluaiLebMue Nang’ and the analysis of ploidystability. ScienceAsia, 33, 437-442.
Surh, Y.J. (2002). Anti-tumor promoting potential of selected spice in- gradients with anti-oxidative and anti-
inflammatoryactivities: a short review. Food Chemical Toxicology, 40, 1091–1097.
Taha H.S., Abbas, M.S., Aly, U.I.,&Gaber, I. (2013).New aspects for callus production, regeneration and molecularcharacterization of Ginger (ZingiberofficinaleRosc.)Medicinal & Aromatic Plants, 6, 2-8.
Taiz, L.,& Zeiger, E. (2002). Mineral nutrition: Plant physiology. 2nd ed. Sinaver Associates Inc. Pub. p. 67-86.
Te-chato, S., Hilae, A.,&Yedum, I. (2002).Improve callus induction and embryogeniccallus formation from cultured young leaves of Oil palm seedling. Thai Journal of Agricultural Science, 35, 407–13.
Tsay, H.,& Tseng, M. (1979).Embryoidformation and plantlet regeneration from anther callus of sweet Potato. Botanical Bulletin AcademiaSinica, 20, 117-122.
Wernicke, W., & Brettell, R. (1980). Somatic embryogenesis from Sorghum bicolorleaf.Nature, 287, 138 -139.
Yam, T.W.,&Arditti, J. (2009). History of orchid propagation: a mirror of the history of biotechnology.
Plant Biotechnology Report, 3, 1-56.
Zapata, E.V., Morales, G.S., Lauzardo, A.N.H., Bonfil, B.M., Tapia, G.T., Sanchez, A.D.J., Valle, M.V.D.,&Aparicio A.J. (2003).In vitroregeneration and acclimatization of plants of Turmeric (Curcuma longa L.)
in a hydroponic system.Biotechnology Applied Biochemistry, 20, 25-31.
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2017-01-10
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